ライフサイエンスコーパス: AUF1

1 AUF1 (also known as heterogeneous nuclear ribonucleoprot

2 AUF1 binds and strongly activates the transcription prom

3 AUF1 binds the SDF-1, alpha-SMA, TGF-beta1, and IL-6 mRN

4 AUF1 has been shown to recruit the exosome to mRNAs.

5 AUF1 is a target of beta-TrCP, the substrate recognition

6 AUF1 is an AU-rich element (ARE)-binding protein that re

7 AUF1 is an RNA-binding protein that targets mRNAs contai

8 AUF1 overexpression preferentially suppressed D allele m

9 AUF1 protein, a negative regulator of ARE mRNA stability

10 AUF1 proteins are shown here to provide direct interacti

11 AUF1 proteins bind the ARE, undergo shuttling, and promo

12 AUF1, TIA-1, and HuR mRNAs were not systematically dysre

13 AUF1-deficient mice undergo striking telomere erosion, m

14 AUF1-mRNA association is a dynamic paradigm directed by

15 RBP AU-binding factor 1 (AUF1) has four isoforms resulting from alternative splic

16 The RBP AU-rich-binding factor 1 (AUF1) isoform p37 was found to have high affinity for th

17 einase cleavage is AU-rich binding factor 1 (AUF1), a cellular protein that binds to AU-rich elements

18 proteins, such as AU-rich binding factor 1 (AUF1), that increase Pth mRNA stability, was increased,

19 AU-rich element RNA-binding protein 1 (AUF1) binding to AU-rich elements (AREs) in the 3'-untra

20 AU-rich element RNA-binding protein 1 (AUF1) regulates the stability and/or translational effic

21 I and AU-rich element RNA-binding protein 1 (AUF1), respectively, with an opposite consequence to the

22 In addition, AUF1 promoted Mef2c gene transcription via a lesser-know

23 Although all AUF1 isoforms generated oligomeric complexes on ARE subs

24 pre-mRNAs, and have discovered that altering AUF1 levels alone only modifies the levels of subsets of

25 cribe isoform-specific characteristics among AUF1 ribonucleoprotein complexes, which likely constitut

26 to elucidate a signature motif shared among AUF1 target transcripts.

27 ometry to identify 14-3-3sigma protein as an AUF1-interacting protein.

28 We recently found chaperone Hsp27 to be an AUF1-associated ARE-binding protein required for tumor n

29 , another anti-apoptotic gene, containing an AUF1 binding site in its 3'-UTR was also increased in CD

30 he ARE-binding protein AUF1, we developed an AUF1 knockout mouse.

31 ough inhibiting the AKT protein kinase in an AUF1-dependent manner.

32 Surprisingly, expression of an AUF1-RGG peptide reduced endogenous VEGF protein levels

33 alizing with the translational apparatus and AUF1 with the exosome.

34 hosphomimetic mutant forms of both Hsp27 and AUF1.

35 intestinal epithelial cells through HuR and AUF1 and provide new insight into the molecular function

36 e show that JunD mRNA is a target of HuR and AUF1 and that polyamines modulate JunD mRNA degradation

37 RNA-binding proteins HuR and AUF1 associate with labile mRNAs bearing AU-rich element

38 RNA-binding proteins HuR and AUF1 bind to many common AU-rich target mRNAs and exert

39 cyclin D1, we provide evidence that HuR and AUF1 can bind target transcripts on both distinct, nonov

40 the functional interactions between HuR and AUF1 have not been systematically studied.

41 suggesting that the balance between HuR and AUF1 is likely important in control of short lived mRNA

42 d a slightly elevated mRNA level for HuR and AUF1 mRNA.

43 ereas the cytoplasmic levels of both HuR and AUF1 protein increased.

44 ated regulation of mRNA stability by HuR and AUF1 proteins contributes to the observed increase in AT

45 altering the competitive binding of HuR and AUF1 to the JunD 3'-UTR.

46 protein complexes; in the cytoplasm, HuR and AUF1 were found to bind to target mRNAs individually, Hu

47 the global alterations in binding of HuR and AUF1 with target transcripts have a critical role in pos

48 These results indicate that HuR and AUF1, which functionally oppose each other, have general

49 Although hnRNP I and AUF1 can interact with many RNA species and regulate the

50 The interaction between LDH and AUF1 is direct as it can be demonstrated in vitro with p

51 on between the levels of these microRNAs and AUF1 has been identified in various osteosarcoma cell li

52 r, target mRNAs recognized by both miRNA and AUF1 are less abundant upon AUF1 overexpression implying

53 s was reduced, indicating that nucleolin and AUF1 have opposing roles in bcl-2 mRNA turnover.

54 ustrates the opposing roles of nucleolin and AUF1 in regulating bcl-2 mRNA stability.

55 tified multiple isoforms of TIA-1, TIAR, and AUF1 at pI values that spanned nearly 3 pH units.

56 TTP and AUF1 proteins linked to miR-221, whereas TIAR coupled wi

57 clear HuR protein content was unchanged, and AUF1 protein increased slightly after amino acid limitat

58 Furthermore, expression of another AUF1-binding RNA element, derived from the 3'-UTR of c-m

59 As AUF1 is ubiquitinated and degraded by proteasomes, we ad

60 ced by estradiol treatment was identified as AUF1 (A + U-rich RNA-binding factor 1) protein isoform p

61 ve role for AUF1 in poliovirus infection, as AUF1 inhibited viral translation and, ultimately, overal

62 eoprotein D family of proteins also known as AUF1 consists of four isoforms implicated in both nuclea

63 The predicted interactions between AUF1 and target mRNAs were recapitulated in vitro using

64 en RNA binding and mRNA degradation for both AUF1 and Argonaute 2 (AGO2), which is an essential effec

65 ry demonstrated that in the developing brain AUF1 proteins are expressed by proliferating neural prog

66 We show here that in the developing brain AUF1 proteins are expressed in a spatiotemporally define

67 bcl-2 mRNA and recruitment of the exosome by AUF1.

68 ture miRNAs, the lowering of Dicer levels by AUF1 diminished the levels of miRNAs tested, but not the

69 scriptional regulation of MAT1A and MAT2A by AUF1 and HuR in HCC.

70 fector of the myogenesis program promoted by AUF1.

71 describe the collection of RNAs regulated by AUF1 (AU-binding factor 1), an RBP linked to cancer, inf

72 5alpha mRNA revealed that in untreated cells AUF1 strongly reduced GADD45alpha mRNA stability, wherea

73 Upon endotoxin challenge, AUF1 knockout mice display symptoms of severe endotoxic

74 pothesis that Hsp27 phosphorylation controls AUF1 levels to modulate ARE mRNA degradation.

75 1/heterogeneous nuclear ribonucleoprotein D (AUF1) in VEGF gene expression.

76 veral regulatory proteins, including hnRNP D/AUF1, which comprises four isoforms of 37, 40, 42 and 45

77 he heterogeneous nuclear ribonucleoprotein D/AUF1 isoforms.

78 directing inflammatory cytokine mRNA decay, AUF1 destabilizes cell-cycle checkpoint mRNAs, preventin

79 stromal fibroblasts through STAT3-dependent AUF1 induction.

80 o define thermodynamic parameters describing AUF1 ribonucleoprotein (RNP) complex formation across a

81 nsfer-based assays showed that the different AUF1 isoforms remodel bound RNA substrates into divergen

82 quitination and degradation of the different AUF1 protein isoforms.

83 n defined the association mechanisms of each AUF1 isoform for ARE-containing RNA substrates and quant

84 Using purified recombinant forms of each AUF1 protein variant, we used chemical cross-linking and

85 Endogenous AUF1 was observed to relocalize to the cytoplasm of infe

86 Enhanced AUF1 degradation required expression of phosphomimetic m

87 Depending on the specific mRNA examined, AUF1 and AGO2 binding is proportional/cooperative, recip

88 ing degradation of the ARE-mRNA decay factor AUF1 by proteasomes.

89 the ARE-specific, mRNA-destabilizing factor, AUF1.

90 for U-rich RNA despite observations that few AUF1-associated cellular mRNAs contain such extended U-r

91 First, AUF1 lowers the steady-state levels of numerous target R

92 First, AUF1 may regulate its own expression by binding to AREs

93 Two of the five AUF1 mRNA 3'-UTR variants position the translational ter

94 We identify a negative role for AUF1 in poliovirus infection, as AUF1 inhibited viral tr

95 n the adult, suggesting a repressor role for AUF1 proteins during enkephalinergic differentiation.

96 All four AUF1 proteins were found to undergo rapid nucleocytoplas

97 lot analysis indicated that only two of four AUF1 protein isoforms were present in the uterine cytoso

98 roteins such as tristetraprolin and the four AUF1 isoforms.

99 Thus, a single gene, AUF1, links maintenance of telomere length and normal ag

100 ther AU-rich element-binding protein, HNRNPD/AUF1, as well as a related protein, laAUF1.

101 on of AUF1 target mRNAs but also suggest how AUF1 binding could regulate protein and/or microRNA bind

102 We therefore identified AUF1-interacting proteins that may play a role in ARE-mR

103 of the enkephalin (ENK) gene, we identified AUF1.

104 proceeds, contributing to dynamic changes in AUF1 3'-UTR structures during embryogenesis.

105 tal, we have identified a signature motif in AUF1 target mRNAs, have found that AUF1 also associates

106 cessive generations, which can be rescued in AUF1 knockout mice and their cultured cells by resupplyi

107 uclear export is facilitated by sequences in AUF1 exon 7 found in the C-terminal domain of the two la

108 cilitated by RNA-binding proteins, including AUF1.

109 The silencing of HuR increased AUF1 binding to the JunD mRNA, decreased the abundance o

110 tential shuttling activity of the individual AUF1 isoforms have not been previously studied in detail

111 ng poliovirus or human rhinovirus infection, AUF1 is cleaved by the viral proteinase 3CD and that AUF

112 , interaction of Linc-RoR with AUF1 inhibits AUF1 to bind to c-Myc mRNA.

113 Through these interactions, AUF1 lowered DICER1 mRNA stability, since silencing AUF1

114 Here, we isolated AUF1-associated mRNAs by immunoprecipitation of (AUF1-RN

115 d in the C-terminal domain of the two larger AUF1 isoforms.

116 Importantly, lowering AUF1 delayed myogenesis, while ectopically restoring MEF

117 Given that many AUF1 target mRNAs encode muscle-specific factors, we inv

118 stabilizes MAT1A messenger RNA (mRNA), Mat1A-AUF1 ribonucleoprotein, HuR protein, which stabilizes MA

119 iquitin-proteasome activity, and one or more AUF1 proteins are thought to be ubiquitinated.

120 ract in vivo with the AU-rich reporter mRNA, AUF1 may be involved in rapid turnover of mRNAs that lac

121 e interesting questions about the ability of AUF1 isoforms to regulate the mRNA binding and decay-pro

122 its family members as well as the ability of AUF1 proteins to serve as possible physical links betwee

123 Analyses of AUF1 from control and TPA-treated cells indicated that p

124 lin-targeting aptamer AS1411, association of AUF1 with bcl-2 mRNA was increased.

125 is inhibited through proteolytic cleavage of AUF1 by the viral proteinase 3CD.

126 Coexpression of AUF1 represses VEGF-3' UTR reporter expression in RAW-26

127 The consequences of AUF1 binding to 10 predicted target mRNAs were tested by

128 P enhanced ubiquitination and degradation of AUF1 and led to stabilization of reporter mRNAs containi

129 ocks ubiquitination and rapid degradation of AUF1 proteins, whereas its deletion permits ubiquitinati

130 re, we further demonstrate that depletion of AUF1 abolishes the global interaction of miRNAs and AGO2

131 Given the wide distribution of AUF1/2-type proteins among land plants, we propose that

132 Deletion of the RGG domain of AUF1 eliminated the repressive effects of AUF1.

133 of AUF1 eliminated the repressive effects of AUF1.

134 fic factors, we investigated the function of AUF1 in skeletal muscle differentiation.

135 We report a major function of AUF1-it activates telomerase expression, suppresses cell

136 a non-cell-autonomous oncogenic function of AUF1.

137 MV-4-11 cell extracts were immunodepleted of AUF1, the rate of decay of ARE(bcl-2) transcripts was re

138 These results suggest that nuclear import of AUF1 is facilitated by sequences found only in the two s

139 osis by acting as a competitive inhibitor of AUF1, preventing AUF1 from binding to its targets.

140 In contrast, the interaction of AUF1 with the ATF3 mRNA is decreased in histidine-depriv

141 ngs to demonstrate that all four isoforms of AUF1 bind directly to stem-loop IV of the poliovirus 5'

142 These data suggest that certain isoforms of AUF1 can serve as "co-activators" of TTP family protein

143 There are four isoforms of AUF1 that result from alternative splicing of exons 2 an

144 ified four alternatively spliced isoforms of AUF1 which migrated at multiple isoelectric points.

145 Indeed, knockdown of AUF1 impairs LPS-mediated p38 mitogen-activated protein

146 Furthermore, levels of AUF1 protein, which destabilizes MAT1A messenger RNA (mR

147 We show that overexpression of AUF1 in mouse macrophage-like RAW-264.7 cells suppresses

148 erefore determined whether overexpression of AUF1 isoforms promotes ARE-mRNA destabilization and whet

149 ic explanation for the diverse population of AUF1 target mRNAs but also suggest how AUF1 binding coul

150 he repression of the oncogenic potentials of AUF1.

151 etic mutants of Hsp27 promote proteolysis of AUF1 in a proteasome-dependent fashion and render ARE mR

152 Finally, quantitation of AUF1 mRNA 3'-UTR splice variants during murine embryonic

153 ol treatment strongly increased the ratio of AUF1 isoforms p45 to p37.

154 ene transcription via a lesser-known role of AUF1 in transcriptional regulation.

155 ocused on the NF-kappaB pathway in search of AUF1-regulated targets.

156 siRNA silencing of AUF1 eliminated the effect of 14-3-3sigma overexpression

157 Thus, the stabilities of AUF1 isoforms are differentially controlled by insertion

158 A subset of AUF1 proteins are shown to directly interact in vitro us

159 hanism to assure co-shuttling of a subset of AUF1 proteins that interact in a heterocomplex.

160 ity of p21(waf1/Cip1/sdi1) mRNA, a target of AUF1 with anti-apoptotic activity, were increased in CDI

161 Here, we identified mRNA targets of AUF1 from a complex pool of cellular mRNAs and examined

162 The C-terminus of AUF1 contains arginine-glycine-glycine (RGG) repeat moti

163 tion to occur and promotes rapid turnover of AUF1 proteins.

164 mRNAs (ARE-mRNAs) requires ubiquitination of AUF1 and its destruction by proteasomes.

165 pre-mRNA splicing produces five variants of AUF1 mRNA that differ in the composition of their 3'-unt

166 -associated mRNAs by immunoprecipitation of (AUF1-RNA) ribonucleoprotein (RNP) complexes from HeLa ce

167 ious biochemical studies showed that optimal AUF1 binding requires 33-34 nucleotides with a strong pr

168 fate (stability, translation) of HuR- and/or AUF1-containing ribonucleoprotein complexes depend on th

169 n of ARE-binding proteins tristetraprolin or AUF1 and proteasome activity, of which the latter has no

170 ls of only four mRNAs, and by overexpressing AUF1, which also lowered the levels of only four mRNAs.

171 eased Dicer expression, while overexpressing AUF1 lowered DICER1 mRNA and Dicer protein levels.

172 ly increased cytoplasmic accumulation of p37 AUF1 and reduced the steady-state level and half-life of

173 Moreover, overexpression of p37 AUF1 restored the ability of cells to rapidly degrade AR

174 hich was eliminated by overexpression of p37 AUF1.

175 Surprisingly, overexpressed p37 AUF1 also destabilized reporter mRNAs containing a nonca

176 Since overexpressed p37 AUF1 could interact in vivo with the AU-rich reporter mR

177 We show that the p37 AUF1 isoform and, to a lesser extent, the p40 isoform po

178 These data indicate that the p37 AUF1 isoform and, to some extent, the p40 isoform are li

179 3sigma binds selectively and strongly to p37 AUF1 and to a lesser extent to the p40 isoform, the two

180 14-3-3sigma therefore binds to p37 AUF1, retains it in the cytoplasm probably by masking it

181 ted with AUF1 and were destabilized in a p37(AUF1)-dependent manner in cells.

182 ere sufficient to nucleate high affinity p37(AUF1) RNP complexes within a larger RNA context.

183 r mRNAs containing minimal high affinity p37(AUF1) target sequences associated with AUF1 and were des

184 nt with the inverse relationship between p37(AUF1) binding affinity and the stability of ARE folding,

185 ciation of the mRNA-destabilizing factor p37(AUF1) was strongly inhibited by adoption of the higher o

186 ed that these contacts were required for p37(AUF1) to remodel local RNA structure.

187 Using the smallest AUF1 isoform (p37(AUF1)) as a model, we employed fluorescence anisotropy-b

188 asts, whereas AUF1 ectopic expression of p37(AUF1) activated these cells and enhanced their paracrine

189 In particular, p37(AUF1) binding to short AU-rich RNA targets was significa

190 itin cycle were found to control p37 and p40 AUF1 protein levels through ubiquitination and proteasom

191 However, p40(AUF1) does not control the TAK1 mRNA levels but instead

192 ession of an RNA interference-refractory p40(AUF1) cDNA restores both signaling pathways.

193 Here, we show that p40(AUF1) serves to maintain proper levels of the kinase TAK

194 Thus, p40(AUF1) regulates a critical node within the NF-kappaB sig

195 define the molecular mechanisms by which p40(AUF1) controls IL10 expression, we focused on the NF-kap

196 The p42 and p45 AUF1 proteins share a C-terminal exon 7 that is not foun

197 terestingly, further validation of predicted AUF1 target transcripts revealed that AUF1 associates wi

198 The predicted AUF1 motif (29-39 nucleotides) contained 79% As and Us,

199 a competitive inhibitor of AUF1, preventing AUF1 from binding to its targets.

200 8 MAP kinase-MK2-Hsp27-beta-TrCP may promote AUF1 degradation by proteasomes and stabilization of cyt

201 A binding proteins, the mRNA decay-promoting AUF1 and the translational suppressor TIAR, were found t

202 antibodies to TNFalpha and IL-1beta protect AUF1 knockout mice against lethal endotoxic shock.

203 The mammalian RNA-binding protein AUF1 (AU-binding factor 1, also known as heterogeneous n

204 The RNA-binding protein AUF1 binds AU-rich elements in 3'-untranslated regions t

205 The ARE-binding protein AUF1 forms a complex with cap-dependent translation init

206 The ubiquitous RNA-binding protein AUF1 promotes the degradation of some target mRNAs, but

207 ted region (UTR) of the mRNA-binding protein AUF1, leading to its down-regulation.

208 tand the function of the ARE-binding protein AUF1, we developed an AUF1 knockout mouse.

209 nt up-regulation of the mRNA-binding protein AUF1, whose promoter contains three canonical STAT3 bind

210 s the p40 isoform of the RNA-binding protein AUF1.

211 xamines the role of the mRNA-binding protein AUF1/heterogeneous nuclear ribonucleoprotein D (AUF1) in

212 at the mRNA-binding, decay-promoting protein AUF1 has higher affinity for allele D mRNA.

213 Another RNA-binding protein, AUF1, regulates target mRNA molecules by enhancing their

214 tion of the AU-rich element-binding protein, AUF1, with GM-CSF mRNA, which determined the rate of dec

215 ntify the AUXIN UP-REGULATED F-BOX PROTEIN1 (AUF1) and its potential paralog AUF2 as important positi

216 tion of the AU-rich element-binding proteins AUF1 and hnRNP C with GM-CSF mRNA, accelerating or slowi

217 e the AU-rich element (ARE) binding proteins AUF1 and HuR.

218 experiments revealed 2 ARE-binding proteins, AUF1 and HuR, associated with IL-8 mRNA in saliva.

219 we report that the RNA-binding protein (RBP) AUF1 (AU-binding factor 1) associates with the endogenou

220 Systematic testing of the TTR-RBPs AUF1, HuR, KSRP, NF90, TIA-1, and TIAR led to three key

221 ng body of data demonstrating that two RBPs, AUF1 and HuR, can antagonistically affect the posttransc

222 impairment of myogenesis seen after reducing AUF1 levels.

223 erogeneity in 3'-UTR sequence could regulate AUF1 expression by two potential mechanisms.

224 stent with the AU-rich sequences of reported AUF1 targets.

225 mportantly, 10 out of 15 previously reported AUF1 target mRNAs contained the AUF1 motif.

226 mice and their cultured cells by resupplying AUF1 expression.

227 Through its actions on target RNAs, AUF1 preserves genomic integrity, in agreement with the

228 teins among land plants, we propose that SCF(AUF1/2) provides additional cross talk between auxin and

229 Second, AUF1 does not change the abundance of many target RNAs,

230 These data indicate that selective AUF1 phosphorylation may regulate ARE-directed mRNA turn

231 and Upf2 can associate with an NMD-sensitive AUF1 mRNA 3'-UTR variant in cells.

232 showed that the expression of NMD-sensitive AUF1 mRNAs is specifically enhanced as development proce

233 a c-Myc mRNA-binding protein, and sequesters AUF1 from binding c-Myc mRNA, leading to downregulation

234 dicted target mRNAs were tested by silencing AUF1, which elevated the steady-state levels of only fou

235 wered DICER1 mRNA stability, since silencing AUF1 lengthened DICER1 mRNA half-life and increased Dice

236 ed to a region found only in the two smaller AUF1 isoforms and which overlaps a putative nuclear loca

237 Using the smallest AUF1 isoform (p37(AUF1)) as a model, we employed fluores

238 Consequently, specific AUF1 down-regulation inhibits IL-6-dependent activation

239 Depletion of beta-TrCP stabilized AUF1.

240 After genotoxic stress, AUF1 and TIAR dissociated from the GADD45alpha mRNA, the

241 In summary, AUF1 suppresses miRNA production by reducing Dicer produ

242 e (MAPK)-MK2-Hsp27 signaling axis may target AUF1 destruction by proteasomes, thereby promoting ARE m

243 o-mesenchymal inducer ZEB1 through targeting AUF1, which binds the 3'-UTR of the ZEB1 mRNA and reduce

244 er ARE-binding protein, hnRNP D (also termed AUF1), which in vivo recognizes AUUUA repeats found in c

245 cleaved by the viral proteinase 3CD and that AUF1 can interact with the long 5' NCR of these viruses

246 Our findings also demonstrate that AUF1 functions as an antiviral factor during infection b

247 These findings demonstrate that AUF1 regulates VEGF expression, and this study identifie

248 RNA gel shift analyses established that AUF1 (hnRNP D) binds to the PCK-7, PCK-6, and PCK-2 segm

249 Additionally, we present clear evidence that AUF1 promotes mesenchymal features in osteosarcoma cells

250 motif in AUF1 target mRNAs, have found that AUF1 also associates with the corresponding pre-mRNAs, a

251 olysome distribution analysis, we found that AUF1 associated with the 3' untranslated region (UTR) of

252 on IL10 mRNA stability, we hypothesized that AUF1 controls the expression of signaling proteins.

253 ndant upon AUF1 overexpression implying that AUF1 is a decay-promoting factor influencing multiple st

254 Our findings indicate that AUF1 functions in promoting miRNA-mediated mRNA decay gl

255 oprecipitation (RIP) analysis indicated that AUF1 binds prominently to Mef2c (myocyte enhancer factor

256 Mutational analysis indicated that AUF1 binds to a UUAUUUUAU sequence within PCK-6 and the

257 dicted AUF1 target transcripts revealed that AUF1 associates with both the pre-mRNA and the mature mR

258 ngle-molecule-binding analyses revealed that AUF1 promoted let-7b loading onto Argonaute 2 (AGO2), th

259 Single-molecule analysis revealed that AUF1 slowed down assembly of AGO2-let-7b-mRNA complex un

260 ecipitation (PAR-CLIP) analysis reveals that AUF1 primarily recognizes U-/GU-rich sequences in mRNAs

261 et RNAs, but ribosome profiling reveals that AUF1 promotes the translation of numerous mRNAs in this

262 We show that AUF1 normally functions to protect against the lethal pr

263 n of the cyclin B mitotic marker showed that AUF1 does not affect root cell division but promotes cyt

264 We have also shown that AUF1 binds to and stabilizes the mRNA of the AKT activat

265 Previously, we have shown that AUF1 facilitates miRNA loading to Argonaute 2 (AGO2), th

266 Our data suggest that AUF1 regulates apoptosis by altering mRNA turnover.

267 The AUF1 mRNA level in roots is strongly up-regulated by aux

268 The AUF1 proteins are largely nuclear but also are found in

269 We refer to this protein assembly as the AUF1- and signal transduction-regulated complex, ASTRC.

270 sly reported AUF1 target mRNAs contained the AUF1 motif.

271 Defects in the AUF1 post-transcriptionally controlled pathway may be in

272 Mutagenesis of the AUF1 proteins and fusion of polypeptides to a green fluo

273 Nucleocytoplasmic shuttling of the AUF1 proteins is shown to utilize a novel arrangement of

274 However, the association of the AUF1-mRNA interaction with decreased ARE-mRNA stability

275 ves genomic integrity, in agreement with the AUF1-elicited prevention of premature cellular senescenc

276 Third, AUF1 unexpectedly enhances the steady-state levels of se

277 suppress this prometastatic process through AUF1 repression.

278 Thus, AUF1 and AGO2 present mRNA-specific allosteric binding r

279 Thus, AUF1 binds to multiple destabilizing elements within the

280 several RNA-binding proteins including TIAR, AUF1, CBF-A, RBM3, heterogeneous nuclear ribonucleoprote

281 nstrated that the RNA-binding proteins TIAR, AUF1, HuR, and TIA-1 all form an RNA-protein complex wit

282 r mechanism linking Hsp27 phosphorylation to AUF1 degradation by proteasomes.

283 which coupled with RNA-binding proteins TTP, AUF1, and TIAR at the 3'-untranslated region to arrest p

284 y both miRNA and AUF1 are less abundant upon AUF1 overexpression implying that AUF1 is a decay-promot

285 In mouse C2C12 myocytes, where AUF1 levels rise at the onset of myogenesis and remain e

286 ation of breast stromal fibroblasts, whereas AUF1 ectopic expression of p37(AUF1) activated these cel

287 lly suppressed D allele mRNA levels, whereas AUF1 silencing selectively elevated D allele mRNA levels

288 novel post-transcriptional mechanism whereby AUF1 acts as a crucial attenuator of the inflammatory re

289 romotes ARE-mRNA destabilization and whether AUF1 isoforms are limiting components for ARE-mRNA decay

290 findings uncover a novel mechanism by which AUF1 binding and transfer of microRNA let-7 to AGO2 faci

291 For most mRNAs in which AUF1 affects their decay rates, mRNA degradation require

292 F Box (SCF) ubiquitin ligases assembled with AUF1/2.

293 y p37(AUF1) target sequences associated with AUF1 and were destabilized in a p37(AUF1)-dependent mann

294 he levels of JunD transcript associated with AUF1, thus stabilizing JunD mRNA.

295 ncer-related pathways whose association with AUF1 and/or HuR were altered when comparing immortalized

296 n with HuR and enhanced its association with AUF1, resulting in an inhibition of JunD expression.

297 over, polysomal LDH exists in a complex with AUF1 and hsp-70, which has been implicated previously in

298 ely non-overlapping tissue distribution with AUF1 and was predominantly expressed in the liver and te

299 Upon energy stress, FILNC1 interacts with AUF1, a c-Myc mRNA-binding protein, and sequesters AUF1

300 to c-Myc mRNA, interaction of Linc-RoR with AUF1 inhibits AUF1 to bind to c-Myc mRNA.