ライフサイエンスコーパス: M. genitalium

1 ational repair pathway plays a minor role in M. genitalium.

2 tly more common among subjects infected with M. genitalium.

3 Inflammation was highest among subjects with M. genitalium.

4 s conserved in other bacteria are missing in M. genitalium.

5 o model and assess endocervical infection by M. genitalium.

6 . vaginalis, and 47 (9.5%) were positive for M. genitalium.

7 es of four geographically diverse strains of M. genitalium.

8 ly conserved in other bacteria are absent in M. genitalium.

9 and widely used to study the epidemiology of M. genitalium.

10 of MG_454 resists organic hydroperoxides in M. genitalium.

11 n-house DNA-based PCR assay for detection of M. genitalium.

12 ermine minimum inhibitory concentrations for M. genitalium.

13 ypes and the MG309 repeats for genotyping of M. genitalium.

14 peroxide when compared to wild-type virulent M. genitalium.

15 U were infected with both C. trachomatis and M. genitalium.

16 lest sequenced prokaryotic genome except for M. genitalium.

17 srupt the cytadherence-related gene mg218 of M. genitalium.

18 nvestigating the biology and pathogenesis of M. genitalium.

19 ith total RNA isolated from M. pneumoniae or M. genitalium.

20 limited availability of diagnostic tests for M. genitalium.

21 and 7 of 10 (70%) participants positive for M. genitalium.

22 We observed a high prevalence of M. genitalium (7.4%) among HIV-infected New Orleans wome

23 s a tool to analyze the function of genes in M. genitalium, a plasmid that replicates in Escherichia

24 terested in defining the mechanisms by which M. genitalium adheres to and colonizes host cell surface

25 with a 3.5-fold increase in odds of incident M. genitalium (adjusted odds ratio = 3.49, 95% confidenc

26 Mice infected with M. genitalium also developed specific antibodies to the

27 Screening and treatment of M. genitalium among HIV-infected individuals may be warr

28 lable molecular methods for the diagnosis of M. genitalium and assays to predict the antibiotic susce

29 Prevalence of M. genitalium and associated genotypic markers of macrol

30 e recently developed assay can test for both M. genitalium and azithromycin resistance mutations at t

31 V)-infected women for an association between M. genitalium and cervicitis, a putative mechanism for e

32 cally diverse clinical sites were tested for M. genitalium and for Chlamydia trachomatis, Neisseria g

33 M. genitalium and H. influenzae belong to two ancient ba

34 growing evidence for an association between M. genitalium and HIV genital shedding and the high prev

35 he strong epidemiologic associations between M. genitalium and human immunodeficiency virus (HIV), pr

36 tions in the completely sequenced genomes of M. genitalium and its close relative M. pneumoniae were

37 that groups of functionally related genes in M. genitalium and M. pneumoniae are often preceded by pr

38 nes, many of which appear to be essential in M. genitalium and other bacteria.

39 Samples were tested for M. genitalium and other STI organisms (Chlamydia trachom

40 ging data demonstrate an association between M. genitalium and PID, and limited data suggest associat

41 leans women, we retrospectively screened for M. genitalium and quantitatively characterized several m

42 carriers are about twofold more prevalent in M. genitalium and Synechocystis.

43 The M. genitalium and T. vaginalis detection rates among 755

44 The associations between M. genitalium and the detection and quantity of genital

45 cells expressing specific human TLR, viable M. genitalium and the recombinant C-terminal portion of

46 d pathways involved in innate recognition of M. genitalium and the response to acute infection in the

47 ervical EC are immunologically responsive to M. genitalium and to purified rMG309c via highly express

48 xpansion of the STI analyte panel (including M. genitalium) and additional specimen source sampling w

49 anococcus jannaschii, Mycoplasma pneumoniae, M. genitalium, and Synechocystis PCC6803.) These repeats

50 redibly small-two to four times smaller than M. genitalium-and these tiny genomes have raised questio

51 relationships of the PDB domains that match M. genitalium are described in the structural classifica

52 Because B. burgdorferi and M. genitalium are distantly related eubacteria, we sugge

53 65 to 350 of the 480 protein-coding genes of M. genitalium are essential under laboratory growth cond

54 discovery, microbiology, and recognition of M. genitalium as a pathogen, and then summarize the rece

55 These findings implicate M. genitalium as an etiologic agent of cervicitis in HIV

56 ug Administration-approved clinical test for M. genitalium available in the United States at this tim

57 ibited a range of antibody responsiveness to M. genitalium based upon ELISA and immunoblot assessment

58 upstream of these two genes was detected in M. genitalium but not M. pneumoniae.

59 he small genome organisms, H. influenzae and M. genitalium, but is totally lacking in H. pylori, Syne

60 ces cumulative PID incidence in women due to M. genitalium by 31.1% (95% range:13.0%-52.0%) over 20 y

61 hat MsrA affects the virulence properties of M. genitalium by modulating its interaction with host ce

62 from women every other month were tested for M. genitalium by nucleic acid amplification testing.

63 cts were also positive for M. fermentans and M. genitalium by PCR.

64 s to Statens Serum Institut for detection of M. genitalium by polymerase chain reaction between 1 Jan

65 generation of genetic diversity observed in M. genitalium by the mutual exchange of sequences makes

66 ed field, the positive predictive values for M. genitalium, C. trachomatis, N. gonorrhoeae, and T. va

67 Here, we report that M. genitalium can establish long-term infection of human

68 gs provide strong experimental evidence that M. genitalium can establish long-term infection of repro

69 The evidence that M. genitalium causes epididymo-orchitis, proctitis, and

70 into wild-type hemadsorption-positive (HA+) M. genitalium cells permitted the isolation of HA- (stra

71 Additionally, the isolation of single-colony M. genitalium clonal variants containing alternative mgp

72 i RNA from five M. pneumoniae clones and two M. genitalium clones indicated that transcription origin

73 hase inflammatory responses and suggest that M. genitalium colonization of reproductive tract tissues

74 M. genitalium colonizes host cells primarily through adh

75 (HIV) infection revealed 19 (18.6%) cases of M. genitalium, commonly (58%) in rectal samples.

76 An msrA disruption mutant of M. genitalium, constructed through homologous recombinat

77 ys (ELISAs) and immunoblot and PCR assays in M. genitalium culture-positive women over 1 to 3 years o

78 re was no evidence of an association between M. genitalium detection or quantity and either plasma HI

79 A 10.0% M. genitalium detection rate from other facilities excee

80 sing the search terms Mycoplasma genitalium, M. genitalium, diagnosis, and detection.

81 Typing assigned M. genitalium DLSTs to 2 major clusters, broadly distrib

82 Libraries of M. pneumoniae and M. genitalium DNA constructed in pGFPUV2 and transformed

83 ative polymerase chain reaction specific for M. genitalium DNA on samples 14-100 days post-treatment.

84 ormat and performance indices of a number of M. genitalium DNA- and RNA-based amplification assays; m

85 ense and inflammation signature activated by M. genitalium during acute infection (48 hours after ino

86 tudy, we demonstrate that surface-associated M. genitalium EF-Tu (EF-Tu(Mg)), in spite of sharing 96%

87 e stress response regulator in the genome of M. genitalium, elevated expression of MG_454 due to phys

88 tions, understanding the mechanisms by which M. genitalium elicits mucosal inflammation is an essenti

89 arative genomics has revealed that MG_454 of M. genitalium encodes a protein with putative function a

90 of identified STI involved sole detection of M. genitalium Expansion of the STI analyte panel (includ

91 Here, we show that M. genitalium expresses N-terminally truncated RecA isof

92 Recovery of viable M. genitalium from lower genital tract specimens was imp

93 CVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by a

94 Although the M. genitalium gene complement is indeed the smallest amo

95 We found that 240 M. genitalium genes have orthologs among the genes of H.

96 ted the set of orthologs with the respective M. genitalium genes.

97 f chaperone expression]) is predicted in the M. genitalium genome as well as three copies of its corr

98 Approximately 4% of the limited M. genitalium genome contains repeat sequences termed Mg

99 city of recombination genes conserved in the M. genitalium genome.

100 Several novel M. genitalium genotypes were identified, all of which we

101 Disruption of some genes accelerated M. genitalium growth.

102 ence of known cervical pathogens, women with M. genitalium had a 3.3-fold greater risk (95% confidenc

103 ke Escherichia coli, inactivation of recA in M. genitalium had a minimal effect on survival after exp

104 Testing for M. genitalium has become important for clinicians treati

105 ng available, and their use in screening for M. genitalium has been advocated, but M. genitalium's na

106 Detection of M. genitalium has relied almost entirely on PCR amplific

107 It appears plausible that M. genitalium has the ability to generate unlimited vari

108 Since M. genitalium has the capacity to invade eukaryotic cell

109 which provides insights into the dynamics of M. genitalium-host interactions.

110 of MG_454 to respond to oxidative stress in M. genitalium implies the absence of a known oxidative s

111 screening programs and targeted treatment of M. genitalium improve reproductive outcomes in women are

112 gpB sequences vary within a single strain of M. genitalium in a pattern consistent with recombination

113 comprehensive testing programs would detect M. genitalium in a significant proportion of females, pa

114 ata which directly implicate the presence of M. genitalium in disease pathogenesis.

115 g and the high prevalence and persistence of M. genitalium in this population suggest that further re

116 sensitive specimen type for the detection of M. genitalium in women.

117 evealed reinfection by a different strain of M. genitalium, indicating the absence of protective immu

118 a high azithromycin failure rate (39%) in an M. genitalium-infected cohort in association with high l

119 The mean age of the M. genitalium-infected females (24.7 years) was lower th

120 Consecutive eligible M. genitalium-infected men and women attending the Melbo

121 Analysis of 31 M. genitalium-infected patient specimens and 7 American

122 e strain and in sequential specimens from an M. genitalium-infected patient.

123 At baseline, 16.1% had prevalent M. genitalium infection and 40.4% had prevalent BV.

124 sequence variation in patients with chronic M. genitalium infection and to analyze the sequence stru

125 lyses assessing associations with persistent M. genitalium infection are needed.

126 as employed to estimate the odds of incident M. genitalium infection at follow-up visits among women

127 Therefore, persistent M. genitalium infection could have important consequence

128 Here, we assessed the incidence of M. genitalium infection in patients attending a sexually

129 tively and quantitatively assess patterns of M. genitalium infection in women attending a sexually tr

130 tis and N. gonorrhoeae infections, while the M. genitalium infection rate in males was significantly

131 Cure of M. genitalium infection resulted in ablation of all sign

132 ourse during menses were less likely to have M. genitalium infection than those who did not (odds rat

133 Chronic M. genitalium infection was associated with increased se

134 rial STI (C. trachomatis, N. gonorrhoeae, or M. genitalium infection) was lower in the intervention a

135 e infection, and 0.66 [95% CI, .38-1.15] for M. genitalium infection).

136 ort female pig-tailed macaques as a model of M. genitalium infection, persistence, and immune evasion

137 gle female pig-tailed macaque as a model for M. genitalium infection, we cervically inoculated eight

138 suitability of a pig-tailed macaque model of M. genitalium infection, we inoculated a pilot animal wi

139 ggests that BV may enhance susceptibility to M. genitalium infection.

140 utinely screening any defined population for M. genitalium infection.

141 There were 59 incident M. genitalium infections among 50 women, for an incidenc

142 estigations into the causal relationships of M. genitalium infections and clinical disease have been

143 ation of heretofore-unrecognized patterns of M. genitalium infections in clinical and experimental sa

144 ively whether screening for and treatment of M. genitalium infections in women and their sexual partn

145 Significant risk factors for M. genitalium infections were black race, younger age, n

146 Female M. genitalium infections were significantly more prevale

147 prove useful in management of some resistant M. genitalium infections, although it is not likely to a

148 prevent the emergence of ever more resistant M. genitalium infections.

149 tion, have been the treatments of choice for M. genitalium infections.

150 Antimicrobial-resistant M. genitalium is a significant problem and may require c

151 In this paper, we show that cytadherence in M. genitalium is affected by an unrelated protein known

152 Because M. genitalium is associated with both HIV acquisition an

153 Although M. genitalium is considered a leading cause of genitouri

154 plasma hominis and the attachment protein of M. genitalium, is an integral membrane protein.

155 le region of MG192 was amplified by PCR from M. genitalium isolates obtained at various time points p

156 This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-t

157 have previously shown that a mutant form of M. genitalium lacking methionine sulfoxide reductase A (

158 -negative nongonococcal urethritis and other M. genitalium-linked infectious etiologies has been very

159 MgpB and MgpC, but was insufficient to clear M. genitalium lower tract infection.

160 Testing of high-risk symptomatic women for M. genitalium may be warranted.

161 Some studies suggest that M. genitalium may increase the risk of HIV acquisition.

162 ity of the third gene (MG192 or mgpC) of the M. genitalium MgPa adhesion operon, demonstrated that th

163 An M. genitalium mutant lacking the MG491 segment correspon

164 fold more likely to shed HIV-1 DNA than were M. genitalium-negative women (adjusted OR, 2.9 [95% conf

165 to arise from the introduction of diagnostic M. genitalium nucleic acid amplification testing includi

166 ns are needed to better define the impact of M. genitalium on women's reproductive health.

167 ates among subjects with monoinfections with M. genitalium or C. trachomatis compared to women with n

168 Women with high M. genitalium organism burdens (more than the median of

169 confidently predict the existence of viable M. genitalium organisms in cervical and vaginal samples.

170 hich at most time points the median ratio of M. genitalium organisms to host cells was </=10, indicat

171 nd sequence analysis of single-colony cloned M. genitalium organisms.

172 ion (STI), 35.9% exhibited sole detection of M. genitalium (P </= 0.0004 versus sole detection of oth

173 1.4% identity with the M. pneumoniae P30 and M. genitalium P32 cytadhesins, respectively.

174 -tailed macaque is a suitable model to study M. genitalium pathogenesis, antibody-mediated selection

175 ndicate that MsrA plays an important role in M. genitalium pathogenicity, possibly by protecting myco

176 In the upper tract, more than 90% of M. genitalium PCR-positive samples were from the uterus

177 Viable M. genitalium persisted in the lower genital tract for 8

178 Genetic diversity of M. genitalium populations was determined to infer whethe

179 , 16.8% of women and 15.1% of men were found M. genitalium positive.

180 stance was detected in 38% (385/1008) of the M. genitalium-positive patients, and the highest rate wa

181 assess macrolide antibiotic resistance among M. genitalium-positive subjects.

182 Among the M. genitalium-positive women, the relative sensitivities

183 Our results also suggest that M. genitalium possesses an active nucleotide excision re

184 Surveillance of M. genitalium prevalence and antimicrobial resistance pa

185 M. genitalium prevalence rates were 16.1% for females an

186 Among 217 men, M. genitalium prevalence was 16.7% (95% confidence inter

187 ve such a set, we compared the 468 predicted M. genitalium protein sequences with the 1703 protein se

188 Herein, we identify 382 of the 482 M. genitalium protein-coding genes as essential, plus fi

189 We conclude that M. genitalium RecA has a primary role in mgpB/C-MgPar re

190 Positive M. genitalium results were confirmed by repeat testing o

191 treatment, and public health significance of M. genitalium reviewed at the meeting is described in de

192 Therefore, M. genitalium RNase R can precisely remove the 3'-traile

193 that the only exoribonuclease identified in M. genitalium, RNase R, is able to remove tRNA 3'-traile

194 pand these studies by examining the roles of M. genitalium ruvA and ruvB homologs.

195 d to improve understanding of key aspects of M. genitalium's natural history before it will be possib

196 ng for M. genitalium has been advocated, but M. genitalium's natural history is poorly-understood, ma

197 However, there is important uncertainty in M. genitalium's natural history parameters, leading to u

198 and behavioural studies to better understand M. genitalium's natural history, and then examined the e

199 Consideration of the cost-effectiveness of M. genitalium screening interventions may be warranted.

200 These data suggest that M. genitalium selectively regulates a limited number of

201 PDB sequences (274) match all of 106 M. genitalium sequences and some parts of another 85; th

202 ion, we show that the domains in the matched M. genitalium sequences come from 114 superfamilies and

203 These results show a high prevalence of M. genitalium single infections, a lower prevalence of c

204 nce variation occurred during the process of M. genitalium single-colony cloning.

205 M. genitalium-specific IgG, but not IgA, was detected in

206 M. genitalium-specific serum antibodies targeting the im

207 ts, MG192 sequences were more related within M. genitalium specimens from an individual patient than

208 italium virulence, we compared the wild-type M. genitalium strain (G37) with an msrA mutant (MS5) str

209 organism, we examined mgpB variation within M. genitalium strain G-37 and observed sequence heteroge

210 infection, we inoculated a pilot animal with M. genitalium strain G37 in the uterine cervix and in sa

211 specimens from two chimpanzees infected with M. genitalium strain G37.

212 rent mgpB variants within a single infecting M. genitalium strain, confirming that mgpB heterogeneity

213 192 gene is highly variable among and within M. genitalium strains in vitro and in vivo, and identifi

214 bacterial pathogens, genomic diversity among M. genitalium strains worldwide is unknown.

215 quences, as well as mgpB variability between M. genitalium strains, suggests that mgpB and MgPar sequ

216 These correlates differ from those found in M. genitalium studies conducted with FSW from West Afric

217 served except with superphysiologic loads of M. genitalium, suggesting that persistent infection occu

218 age in a 5-year period where all diagnostic M. genitalium testing in Denmark was centralized at the

219 establish the cost-effectiveness of routine M. genitalium testing in symptomatic patients and screen

220 health agencies should consider integrating M. genitalium testing into the management of persons wit

221 The aim of the study was to analyze the M. genitalium testing pattern and distribution of positi

222 dom use were more likely to be infected with M. genitalium than those who reported less frequent use

223 In M. genitalium, this transcription terminates at two clos

224 We conclude that the M. genitalium TMA and PCR assays are highly specific and

225 Incorporation of M. genitalium TMA into comprehensive testing programs wo

226 investigated the transcriptional response of M. genitalium to elevated temperatures and detected the

227 This study highlights the capacity of M. genitalium to elicit cervical inflammation and, consi

228 Attachment of M. genitalium to the host cell's apical surface was obse

229 In addition, dissemination of M. genitalium to the knee tissues was observed as early

230 ch the sequences of the 467 gene products of M. genitalium to the sequences of the domains that form

231 In this study, we found that an M. genitalium transposon mutant that lacks expression of

232 diol and then inoculated intravaginally with M. genitalium type strain G37 or a contemporary Danish s

233 e than a single 1g dose at achieving cure of M. genitalium urethritis and importantly did not reduce

234 lide and new approaches for the treatment of M. genitalium urethritis are required.

235 obiological cure was determined for men with M. genitalium urethritis treated with azithromycin 1.5g

236 of this study was to investigate if and how M. genitalium uses a minimal genome to generate genetic

237 ed were tested for detection and quantity of M. genitalium using polymerase chain reaction analysis.

238 s into the mechanisms by which MsrA controls M. genitalium virulence, we compared the wild-type M. ge

239 The prevalence of M. genitalium was 12.9%.

240 M. genitalium was also less prevalent among FSW who had

241 prior urethritis, and chlamydial infection, M. genitalium was associated with a 6.5-fold increased r

242 unclear, and we sought to determine whether M. genitalium was capable of activating innate immune re

243 at replicates in Escherichia coli but not in M. genitalium was constructed to disrupt the cytadherenc

244 ase chain reaction (PCR) in 154 women (51%); M. genitalium was detected by qualitative PCR in 52 (17%

245 Among the women, M. genitalium was detected by the TMA and PCR assays in

246 M. genitalium was detected in 10.5% of stored specimens

247 Among the men, M. genitalium was detected in 24 urine specimens (6.8%)

248 M. genitalium was detected in 27 (22%) of 121 NGU case p

249 M. genitalium was detected in 282 (11.4%) patients.

250 M. genitalium was detected in 50 (7.0%) of 719 women.

251 M. genitalium was independently associated with detectio

252 tion-PCR showed that expression of MG_454 in M. genitalium was not elevated in response to oxidative

253 als inoculated in salpingeal pockets, viable M. genitalium was recovered for 2 weeks.

254 Using a novel quantitative PCR assay, M. genitalium was shown to replicate from 0 to 80 days p

255 e of 22 (41%; 95% CI, 20%-62%) patients with M. genitalium were infected with DLSTs possessing genoty

256 Three Mn-binding proteins (MnBPs) of M. genitalium were isolated by affinity chromatography.

257 and douching were positively associated with M. genitalium, whereas bacterial vaginosis and cunniling

258 iptional organization of the RuvAB system of M. genitalium, which is cotranscribed with two novel ope

259 t cells, MG_186 has the potential to provide M. genitalium, which possesses the smallest genome of an

260 of adherence, we examined the interaction of M. genitalium with a primary component of the mucosal ep

261 ecimens from 28 958 patients were tested for M. genitalium, with an increasing trend from 3858 per ye