ライフサイエンスコーパス: N. caninum

1 N. caninum DNA was amplified most consistently from brai

2 N. caninum expressing TgGRA15 differentially disturbed t

3 onoclonal antibody (MAb), MAb 4A4-2, against N. caninum tachyzoites.

4 he mechanisms involved in protection against N. caninum-associated abortions.

5 cow sera defined by fetal histopathology and N. caninum immunohistochemistry and by maternal N. canin

6 caninum tachyzoite lysate antigen (NcAg) and N. caninum tachyzoite culture supernatant.

7 ng inoculation consisting of live, avirulent N. caninum tachyzoites followed by virulent challenge du

8 e of exposure of polar bears to C. burnetii, N. caninum, and F. tularensis.

9 cows with abortion confirmed to be caused by N. caninum.

10 rgeted genes of Toxoplasma gondii, driven by N. caninum promoters, have yielded robust expression and

11 In cattle, N. caninum has particular significance as a cause of abo

12 Abundant NcCyP was detected in whole-cell N. caninum tachyzoite lysate antigen (NcAg) and N. canin

13 PCR also detected N. caninum DNA in 6 of 8 fetuses that had typical lesion

14 In fresh or frozen tissues, PCR detected N. caninum DNA in 10 of 13 true-positive fetuses (77%) a

15 ixed paraffin-embedded tissues, PCR detected N. caninum DNA in 13 of 13 true-positive fetuses (100%)

16 we examined the utility of PCR in detecting N. caninum infection in fetal tissues from spontaneous b

17 d to analyze several independent and diverse N. caninum isolates; both antigens were recognized in al

18 eptor-mediated apoptosis is repressed during N. caninum infection, and the data further showed that t

19 gnizes several pathogens and its role during N. caninum infection has not yet been described.

20 ons that coyotes (Canis latrans) can excrete N. caninum oocysts in their feces and that white-tailed

21 es that Nod2-dependent responses account for N. caninum elimination.

22 Current diagnostic methods for N. caninum infection in cattle and the advances necessar

23 Four N. caninum-infected Holstein cattle developed NcSRS2 pep

24 signated Ncp29 and Ncp35, respectively) from N. caninum tachyzoites that are the predominant antigens

25 ive transfer of CD8+ T-cell splenocytes from N. caninum-infected mice was protective against challeng

26 On the other hand, N. caninum expressing TgROP16 induced host STAT3 phospho

27 and versatile method to accurately identify N. caninum infection status in cattle using a single cut

28 In N. caninum tachyzoite culture supernatant, three NcCyP b

29 tive IkappaB kinase activity was detected in N. caninum extracts, thereby implying that this parasite

30 he NF-kappaB subunit p65 was not detected in N. caninum-infected cells, although this host transcript

31 ndicate that heterologous gene expression in N. caninum is a useful tool for the study of specific ge

32 tor subsets indicated that CD4(+) CTL killed N. caninum-infected, autologous target cells and that ki

33 caninum immunohistochemistry and by maternal N. caninum indirect fluorescence assay (IFA) at a 1:200

34 In summary, the modified N. caninum cELISA provided a simple, rapid, and versatil

35 oss-reactive antibodies recognizing multiple N. caninum antigens by immunoblot assay, did not inhibit

36 ions to the cELISA included capturing native N. caninum antigen with a parasite-specific MAb (MAb 5B6

37 paB pathway is not central to the ability of N. caninum to prevent apoptosis of their host cells.

38 Immunoblot analysis of N. caninum tachyzoite antigens with sera from cows with

39 Confirmation of N. caninum infection by immunohistochemistry has low sen

40 PCR detection of N. caninum DNA in formalin-fixed, paraffin-embedded tiss

41 n 6 of 8 fetuses that had typical lesions of N. caninum but were immunohistochemistry negative, indic

42 histochemistry determined the true status of N. caninum infection in each fetus.

43 2 bound diffusely to the exterior surface of N. caninum tachyzoites and recognized a single 65-kDa ba

44 was inhibited by mild periodate treatment of N. caninum antigen, demonstrating the carbohydrate natur

45 4-2 binds a carbohydrate epitope on a single N. caninum tachyzoite surface antigen that is recognized

46 In this study, N. caninum-specific CTL expanded from peripheral blood m

47 se findings support investigation of subunit N. caninum vaccines incorporating NcSRS2 gene sequences

48 crobial protein in the N. caninum tachyzoite N. caninum cyclophilin (NcCyP) as a major component of t

49 These observations demonstrate that N. caninum protects against lethal T. gondii infection b

50 ing this approach, we here demonstrated that N. caninum expressing T. gondii's GRA15 and ROP16 kinase

51 of these parasites and present evidence that N. caninum and H. heydorni are separate species.

52 dy has identified a microbial protein in the N. caninum tachyzoite N. caninum cyclophilin (NcCyP) as

53 e (PVM) of T. gondii was not apparent on the N. caninum PVM.

54 These results indicate that the N. caninum tachyzoite naturally produces a potent IFN-ga

55 The binding of MAb 4A4-2 to N. caninum tachyzoite antigen was consistently inhibited

56 ar apicomplexan protozoan closely related to N. caninum.

57 ion and characterization of CTL responses to N. caninum in the natural, outbred, bovine host will fac

58 decrease in the numbers of mice transmitting N. caninum and a lower frequency of transmission by indi

59 parasite-specific CTL against transplacental N. caninum transmission in cattle.

60 Testing of the 4,323 bovine sera of unknown N. caninum status revealed a distinct bimodal distributi

61 lone, and a set of 4,323 cow sera of unknown N. caninum status.

62 ent study was conducted to establish whether N. caninum is similarly capable of subverting apoptotic

63 high degree of resistance to infection with N. caninum.

64 6) that were infected intraperitoneally with N. caninum were protected against a lethal challenge fro

65 lation in mice that had been vaccinated with N. caninum and challenged with T. gondii was observed.