ライフサイエンスコーパス: S. coelicolor

1 S. coelicolor A3(2), M600 and J1501 possess L-TIRs, wher

2 S. coelicolor membranes were also able to catalyse the t

3 S. coelicolor mutants globally deficient in antibiotic p

4 S. coelicolor PecS, which exists as a homodimer, binds t

5 S. coelicolor PNPase was more effective than its E. coli

6 S. coelicolor produces SapB, an amphipathic peptide that

7 S. coelicolor shows a bias toward one type of genetic in

8 her characterize the Amycolatopsis sp. AA4 - S. coelicolor interaction by examining expression of dev

9 An S. coelicolor msdA mutant, constructed by insertion of a

10 d near the RNase ES termini interact with an S. coelicolor homologue of polynucleotide phosphorylase

11 minal region of RbpA and sigma domain 2, and S. coelicolor RbpA mutants that are defective in binding

12 Data from B. subtilis 3610 and S. coelicolor A3(2) provided a means of validation for t

13 x range of -450 to +80 mV, P. aeruginosa and S. coelicolor SoxR are less sensitive to viologens, whic

14 the reduced sensitivity of P. aeruginosa and S. coelicolor SoxR.

15 of colony biofilms in both P. aeruginosa and S. coelicolor, which shows that "secondary metabolites"

16 s possible to predict operons in E. coli and S. coelicolor with 83% and 93% accuracy respectively, us

17 efficacy as operon predictors in E. coli and S. coelicolor.

18 hat the Streptomyces species S. lividans and S. coelicolor, morphologically complex gram-positive soi

19 enotypic distinction between S. lividans and S. coelicolor.

20 n both the S. coelicolor parental strain and S. coelicolor YL/sgFabH (a deltafabH mutant carrying a p

21 t, we screened FGEs from M. tuberculosis and S. coelicolor against synthetic peptide libraries and id

22 Mycobacterium tuberculosis whiB2 and S. coelicolor whiB complemented the defect in M. smegmat

23 surfactin acts antagonistically by arresting S. coelicolor aerial development and causing altered exp

24 Transduction of plasmid DNA between S. coelicolor and S. verticillus was observed at frequen

25 Constructed disruptions of rnc in both S. coelicolor 1501 and Streptomyces lividans 1326 caused

26 o-ACP was phosphopantetheinylated to 100% by S. coelicolor holo-acyl carrier protein synthase (ACPS),

27 Hydrolysis of PNPP by S. coelicolor PtpA was competitively inhibited by dephos

28 Hydrolysis of PNPP by S. coelicolor PtpA were 0.75 mM (pH 6.0, 37 degrees C) a

29 s to find that deferrioxamine E, produced by S. coelicolor, could be readily utilized by Amycolatopsi

30 ally similar to the SapB peptide produced by S. coelicolor.

31 esis of the multiple antibiotics produced by S. coelicolor.

32 e development of aerial hyphae and spores by S. coelicolor is inhibited by surfactin, a lipopeptide s

33 The presence of L-TIRs in certain S. coelicolor strains represents a major chromosomal alt

34 transposition of a plasmid library of cloned S. coelicolor DNAs.

35 removed or duplicated hundreds of contiguous S. coelicolor genes, altering up to 33% of the chromosom

36 C3 were shown to restore to a SapB-deficient S. coelicolor mutant the capacity to undergo complete mo

37 xtracellular signalling cascade proposed for S. coelicolor and is a member of the bldD extracellular

38 extracellular signaling cascade proposed for S. coelicolor bld mutants.

39 Our data suggests a comprehensive role for S. coelicolor AfsS as a master regulator of both antibio

40 The Ku homolog from S. coelicolor contains a distinct version of the HEH dom

41 gue of M. tuberculosis CmtR (CmtR(Mtb)) from S. coelicolor, denoted CmtR(Sc).

42 r structural zinc, the RbpA orthologues from S. coelicolor and M. tuberculosis share a common structu

43 Mycelial extracts prepared from S. coelicolor cultures incorporated radioactive ATP into

44 Using a sigma factor SigT from S. coelicolor as a model, we successfully expressed and

45 Like its numerous homologues, S. coelicolor RNase J can also cleave some RNA internall

46 In S. coelicolor, the van genes are induced by both vancomy

47 active precursor, modulates SoxR activity in S. coelicolor to stimulate the production of a membrane

48 Overexpression of afsS in S. coelicolor and certain related species causes antibio

49 first time the presence of albaflavenone in S. coelicolor and clearly demonstrate that the biosynthe

50 While sco5745 is the only RNase J allele in S. coelicolor, the gene is not essential.

51 duce the production of the Mm antibiotics in S. coelicolor.

52 vious studies of prodiginine biosynthesis in S. coelicolor support a novel role for RedJ in facilitat

53 al activator of actinorhodin biosynthesis in S. coelicolor, is inhibited by the binding of heptaene,

54 tive regulator of antibiotic biosynthesis in S. coelicolor.

55 ted regulation of antibiotic biosynthesis in S. coelicolor.

56 SDH that is involved in valine catabolism in S. coelicolor.

57 he actinorhodin biosynthetic gene cluster in S. coelicolor.

58 metabolism and morphological development in S. coelicolor and the identification of host-encoded lea

59 n production or morphological development in S. coelicolor, although their mutation could influence t

60 modulating morphological differentiation in S. coelicolor.

61 ailless protein, unlike the other two Dps in S. coelicolor, does not readily oligomerise.

62 onCI, encoding a flavin-linked epoxidase, in S. coelicolor was shown to significantly increase the ab

63 ted foci and other weaker, irregular foci in S. coelicolor vegetative hyphae.

64 ed were substrates, including those found in S. coelicolor extracts, and all yielded several products

65 idues are not essential for DnaE function in S. coelicolor.

66 li survival, rns is not an essential gene in S. coelicolor.

67 x reductase (FDR) proteins coded by genes in S. coelicolor were expressed in Escherichia coli, purifi

68 lly, coexpressing the rifG-N and -J genes in S. coelicolor YU105 under the control of the act promote

69 SoxR regulates five genes in S. coelicolor, including those encoding a putative ABC t

70 the antibiotic-specific regulatory genes in S. coelicolor.

71 n M. tuberculosis and critical for growth in S. coelicolor, these data support a model in which RbpA

72 dence that many of the targets identified in S. coelicolor are also under the control of the sigmaR h

73 th 76% amino acid identity was identified in S. coelicolor, and insertional mutagenesis indicated tha

74 is the third ACP to have been identified in S. coelicolor; the two previously characterized ACPs are

75 are consistent with duplication of GCH II in S. coelicolor promoting evolution of a new function.

76 pt from rpsO-pnp is cleaved by RNase IIIS in S. coelicolor.

77 ferring rifampin-resistance, was isolated in S. coelicolor to provide a genetic marker to follow tran

78 egulated in an RNase III-dependent manner in S. coelicolor.

79 tibiotics and other secondary metabolites in S. coelicolor, suggest that revision of the currently pr

80 rated by the absence of RamS modification in S. coelicolor hyphae treated with the Bacillus subtilis

81 coelicolor, it normally is expressed only in S. coelicolor-generating the deep-blue colonies responsi

82 ly increase the database of known operons in S. coelicolor and provide valuable information for explo

83 biogenesis might be an essential pathway in S. coelicolor.

84 RNA increases during the stationary phase in S. coelicolor and that induction of a plasmid-borne copy

85 read-through transcript normally produced in S. coelicolor.

86 ctors that regulate antibiotic production in S. coelicolor.

87 under the control of its native promoter in S. coelicolor M512, a host lacking the SCP1 plasmid, and

88 PNPase is a cold shock protein in S. coelicolor and the activity of the rpsO-pnp promoters

89 damage and that deletion of this protein in S. coelicolor increases antibiotic production.

90 cance of the redundancy of these proteins in S. coelicolor is not known.

91 w that a representative of these proteins in S. coelicolor possesses a dodecameric quaternary structu

92 Deletion of redJ in S. coelicolor leads to a 75% decrease in prodiginine pro

93 anK is required for vancomycin resistance in S. coelicolor.

94 factors associated with stress responses in S. coelicolor.

95 [3H]-adenosine were used to label the RNA in S. coelicolor cultures of different ages, and total RNA

96 r the initiation of sporulation septation in S. coelicolor.

97 re color, showing that disruption of sigF in S. coelicolor changes the nature of the spore pigment ra

98 While the role of SoxR in S. coelicolor remains under investigation, these studies

99 control the response to disulfide stress in S. coelicolor.

100 nd is a novel regulator of rRNA synthesis in S. coelicolor.

101 are dispensable for growth and viability in S. coelicolor.

102 ed fatty acids were also detected in vivo in S. coelicolor.

103 appropriate intracellular levels of zinc in S. coelicolor.

104 tionally mutated library was introduced into S. coelicolor, and transposon insertions were recovered

105 and to test whether their introduction into S. coelicolor affected antibiotic production.

106 Complementation by only dptI, or its S. coelicolor homologue, glmT, restored the biosynthesis

107 d that a 4.9 Mb central region of the linear S. coelicolor chromosome encodes 'core' functions expres

108 t in the production of actinorhodin, a major S. coelicolor antibiotic.

109 This makes S. coelicolor an advantageous system for the study of ce

110 emperature-sensitive DNA replication mutant, S. coelicolor ts-38.

111 ore genes were downregulated when grown near S. coelicolor, leading us to find that deferrioxamine E,

112 report the genome sequence of PhiCAM, a new S. coelicolor generalized transducing bacteriophage, iso

113 own to significantly increase the ability of S. coelicolor to epoxidize linalool, a model substrate f

114 oarrays to measure globally the abundance of S. coelicolor transcripts in cells growing in liquid med

115 ift assays revealed an increased affinity of S. coelicolor PNPase for the substrate possessing a 3' s

116 chromatography/mass spectrometry analysis of S. coelicolor culture extracts established the presence

117 Here we report the analysis of S. coelicolor WhiD purified anaerobically from Escherich

118 as also capable of restoring the capacity of S. coelicolor and S. tendae bald mutants to erect aerial

119 ce the wild-type allele in the chromosome of S. coelicolor and S. lividans.

120 During stationary phase, the composition of S. coelicolor transcripts appears to shift from large qu

121 chain elongation intermediate to cultures of S. coelicolor CH999/pJRJ2 results in formation of a 16-m

122 is significantly elevated after exposure of S. coelicolor cultures to urate.

123 rbpA expression is induced upon exposure of S. coelicolor to rifampicin and that this, in part, invo

124 Expression of S. coelicolor acpP in Escherichia coli yielded several d

125 After expression of S. coelicolor ptpA in Escherichia coli with a pT7-7-base

126 homogeneity from crude mycelial extracts of S. coelicolor and shown to be BldD.

127 which encodes the principal sigma factor of S. coelicolor.

128 with our previous demonstration that ftsZ of S. coelicolor is not needed for viability, these finding

129 We show that the genome of S. coelicolor encodes for three Dps proteins including a

130 omoters changed over the course of growth of S. coelicolor and studies in three sigma factor mutant s

131 genes expressed during vegetative growth of S. coelicolor cultures, we used DNA microarrays to measu

132 tively constant over the course of growth of S. coelicolor M145, but that on a molar basis, the level

133 alin A (ConA) inhibited phi C31 infection of S. coelicolor J1929, and this could be partially reverse

134 uction of amychelin and in the inhibition of S. coelicolor development.

135 osomal state of the original soil isolate of S. coelicolor A3(2).

136 CE87.05, isolated from the cosmid library of S. coelicolor A3(2).

137 tions, construction of a sigF null mutant of S. coelicolor M145 caused the same change in spore color

138 that all of the characterized bld mutants of S. coelicolor are defective in the regulation of galP1,

139 afsB mutants of S. coelicolor are deficient in the production of both co

140 cedure for generating insertional mutants of S. coelicolor based on in vitro transposition of a plasm

141 de that restored to developmental mutants of S. coelicolor the ability to raise aerial hyphae.

142 Bald mutants of S. coelicolor, which are blocked in aerial mycelium form

143 ubstrate derived from the rpsO-pnp operon of S. coelicolor.

144 bstrates derived from the rpsO-pnp operon of S. coelicolor.

145 prenol phosphate in membrane preparations of S. coelicolor.

146 In the presence of S. coelicolor malonyl CoA:ACP transacylase (MCAT), the r

147 t Streptomyces lividans, a close relative of S. coelicolor and naturally Pgl-, does not contain homol

148 Addition of the gamma-butyrolactone SCB1 of S. coelicolor resulted in loss of the DNA-binding abilit

149 al and essential sigma factor (sigmaHrdB) of S. coelicolor restored Act and Red production in the afs

150 d between spa2 of S. ambofaciens and spaA of S. coelicolor.

151 -Seq, we have examined the transcriptomes of S. coelicolor M145 and an RNase III (rnc)-null mutant of

152 enotype on S. diastatochromogenes but not on S. coelicolor.

153 ing to the approximate mass of the predicted S. coelicolor msdA product (52.6 kDa), and specific MSDH

154 as a substrate in assays employing purified S. coelicolor RNase III.

155 ion of a pentapeptide (HCDLP) to recombinant S. coelicolor NiSOD lacking these N-terminal residues, N

156 he length of the 22 kb TIRs of the sequenced S. coelicolor strain M145.

157 two phylogenetically distant streptomycetes, S. coelicolor A3(2) and S. griseus: (1) gamma -butyrolac

158 perature and osmotic upshifts, we found that S. coelicolor transiently induces a set of several hundr

159 We show here that S. coelicolor desA encodes a novel LDC and we hypothesiz

160 These results indicate that S. coelicolor PecS responds to the ligand urate by atten

161 We also show that S. coelicolor AtrA can bind in vitro to the promoter of

162 in vitro and in vivo experiments showed that S. coelicolor ParB protein interacts specifically with t

163 The S. coelicolor absA locus was defined by four UV-induced

164 The S. coelicolor chromosome contains more parS sequences th

165 The S. coelicolor genome encodes a subgroup of sensor kinase

166 Unlike in other bacteria, the S. coelicolor Dps proteins are not induced in response t

167 predominate (approximately 70%) in both the S. coelicolor parental strain and S. coelicolor YL/sgFab

168 tion of differentiation-related mRNAs by the S. coelicolor AbsB/RNase III enzyme occurs largely by ri

169 lated globally and differentially during the S. coelicolor growth cycle by the RNaseIII homologue Abs

170 mplementing DNA and show that it encodes the S. coelicolor homolog of RNase III (rnc).

171 In marker exchange experiments, the S. coelicolor rnc gene rescued absB mutants, restoring a

172 g the deep-blue colonies responsible for the S. coelicolor name.

173 rth protein was sought and purified from the S. coelicolor CH999 host on the basis of its ability to

174 isolated SigT-interactive proteins from the S. coelicolor lysate based on the tandem affinity purifi

175 st other members of this class, however, the S. coelicolor bkdR gene product serves to repress transc

176 The acceptor subsites +1 to +4 in the S. coelicolor enzyme are well conserved in the M. tuberc

177 re 57 putative GntR family regulators in the S. coelicolor genome) that respond to nutritional and/or

178 for an uridylyltransferase) are found in the S. coelicolor genome, the regulation of the GSI activity

179 ntial operator sites to be identified in the S. coelicolor genome.

180 P450 105D5 are located close together in the S. coelicolor genome.

181 tify 22 likely sigmaU regulon members in the S. coelicolor genome.

182 iology of yet another cryptic protein in the S. coelicolor genome.

183 ed regulation of antibiotic synthesis in the S. coelicolor life cycle.

184 d to be glycosylated with a trihexose in the S. coelicolor parent strain, J1929, but not in the pmt(-

185 bldN, and other key downstream genes in the S. coelicolor transcriptional cascade.

186 Unlike the E. coli soxR deletion mutant, the S. coelicolor equivalent is not hypersensitive to oxidan

187 of the predicted amino acid sequence of the S. coelicolor beta' subunit to those characterized from

188 ted mutagenesis, the carboxy terminus of the S. coelicolor beta' subunit was modified to contain six

189 Disruption of the S. coelicolor dpsA, dpsB and dpsC genes resulted in irre

190 kinetics, and protein crystallography of the S. coelicolor enzyme, we have now identified the +1 to +

191 the E. coli enzyme was ca. twice that of the S. coelicolor enzyme.

192 gene (fabD) encoding another subunit of the S. coelicolor FAS, malonyl coenzyme A:ACP acyl-transfera

193 tematic and comprehensive mutagenesis of the S. coelicolor genome.

194 rresponding to the 4.9 Mb core region of the S. coelicolor M145 chromosome were more highly expressed

195 cleoside diphosphates on the activity of the S. coelicolor PNPase but not the E. coli enzyme.

196 s species that also contain orthologs of the S. coelicolor SerRS.

197 beta' subunits, one of which consists of the S. coelicolor subunit and those from Mycobacterium lepra

198 f the cleavage sites in the stem-loop of the S. coelicolor transcript are more akin to those identifi

199 those obtained by microarray analysis of the S. coelicolor transcriptome and with studies describing

200 acin and ramoplanin were not inducers of the S. coelicolor VanRS system, in contrast to results obtai

201 s of genes immediately adjacent to it on the S. coelicolor chromosome that likely encode an ATP-bindi

202 We exploited this distinction to replace the S. coelicolor vanRS genes with the vanRS genes from S. t

203 ination, indicating the possibility that the S. coelicolor promoter/activator functions appropriately

204 lmL), which exhibits a low similarity to the S. coelicolor SerRS, is hypothesized to play a role in v

205 When the S. coelicolor msdA gene was overexpressed in Escherichia

206 . twofold higher than that observed with the S. coelicolor enzyme.

207 ted interchange of this residue in the three S. coelicolor intragenomic homologues is necessary and s

208 Transfer of the pIPP2 derivatives to S. coelicolor and catechol dioxygenase assays demonstrat

209 levels changed by >/= 2-fold between the two S. coelicolor strains and organized those transcripts in

210 ere analyzed during development of wild-type S. coelicolor on solid medium.

211 ed to sporulating aerial hyphae in wild-type S. coelicolor.

212 triketide 6, compounds 18, 31, and 32, with S. coelicolor CH999/pJRJ2, resulting in the formation of

213 ble macrolactone product when incubated with S. coelicolor CH999/pJRJ2.

214 While mutations that interfere with S. coelicolor development late in its life cycle did not