ライフサイエンスコーパス: S. pombe

1 s, neurons cells from mice, and yeast cells (S. pombe).

2 plays a central role in zinc homeostasis in S. pombe.

3 d examining salt tolerance in sod2-deficient S. pombe.

4 activities between two different proteins in S. pombe.

5 confer salt tolerance when reintroduced into S. pombe.

6 sion yeasts Schizosaccharomyces kambucha and S. pombe.

7 ill be a valuable tool for future studies in S. pombe.

8 integrity and cellular stress resistance in S. pombe.

9 histone expression at the end of S phase in S. pombe.

10 quired for proper cell fate determination in S. pombe.

11 ences that contained 31% of the promoters of S. pombe.

12 en the CPC and the process of cytokinesis in S. pombe.

13 eb in mammals, and Tsc1/Tsc2 inhibit Rhb1 in S. pombe.

14 on similarly as an export carrier of mRNA in S. pombe.

15 tor 1 (Tbf1), a second TRF1/TRF2 ortholog in S. pombe.

16 nstream components of the meiotic pathway in S. pombe.

17 uble mutant generation in the fission yeast, S. pombe.

18 itotic DSB repair and crossover formation in S. pombe.

19 ity of cleaving nicked HJs during meiosis in S. pombe.

20 the hermes transposon from the housefly into S. pombe.

21 epair to ensure accurate nuclear division in S. pombe.

22 -NXT pathway, is required for mRNA export in S. pombe.

23 tial for efficient nuclear protein import in S. pombe.

24 y species and in heterochromatin assembly in S. pombe.

25 Rae1p is an essential mRNA export factor in S. pombe.

26 sulting from loss of Rho1 GTPase function in S. pombe.

27 , for the coordination of DNA replication in S. pombe.

28 E expansion required for "closed" mitosis in S. pombe.

29 REBP activation and low-oxygen adaptation in S. pombe.

30 terest in developing research projects using S. pombe.

31 osaccharomyces japonicus, unlike its role in S. pombe.

32 mitochondrial proteins in S. cerevisiae and S. pombe.

33 related mutant has only a mild phenotype in S. pombe.

34 oles for Smc5/6 are genetically separable in S. pombe.

35 were most homologous to molecules present in S. pombe (52% identical and 67% homologous for PCRan1 an

36 sis also identified 24 SNPs between ours and S. pombe 972h- strain yFS101 that was recently sequenced

37 ations and down-regulated WEE1 (WEE1 homolog-S. pombe), a kinase that blocks cell-cycle progression.

38 Here, we show that in the fission yeast S. pombe, a relatively small number of CENP-A/Cnp1 nucle

39 Systematic analysis of the entire S. pombe ABC transporter family identified Abc2 as a ful

40 The S. pombe abc1 abc2 abc3 abc4 hmt1 quintuple and abc2 hmt

41 , the septation initiation network (SIN), in S. pombe act through an unknown mechanism to keep the ph

42 S. pombe actin shares many properties with skeletal musc

43 report describing an activity that protects S.pombe against the toxic effects of O6-alkylguanine add

44 Heterologous expression of cehmt-1 in S. pombe alleviates the Cd2+-hypersensitivity of hmt- mu

45 The response of S. pombe, also known as fission yeast, to misfolded prot

46 ASs) in two yeast species, S. cerevisiae and S. pombe Although >80% of the mRNA genes in each species

47 cerol and glycerophospholipid homeostasis in S. pombe, analogous to mammalian SREBP-1.

48 -modifying enzymes (a PtdIns(4)P-5-kinase in S. pombe and a PI-4-kinase in D. melanogaster) that inte

49 Whereas studies of Mre11 complex mutants in S. pombe and A. thaliana indicate that the complex has o

50 ghly active in inhibiting PCNA function both S. pombe and human cells and showed a high affinity for

51 tural differences are also conserved between S. pombe and humans, suggesting that the S. pombe struct

52 and S. cerevisiae, but are conserved between S. pombe and humans.

53 esolution map of transcription elongation in S. pombe and identify divergent roles for Spt4 in contro

54 nd process for crossover formation exists in S. pombe and is consistent with our finding that deletio

55 dated three functional repurposing events in S. pombe and mammalian cells and discovered that (1) two

56 ds containing candidate insertion sites into S. pombe and mapped the positions of integration.

57 duplication in Saccharomyces cerevisiae and S. pombe and PcpA, the anchor for gamma-TuSCs at the SPB

58 ous assays of recombination intermediates in S. pombe and provide new information on the mechanism of

59 er, our data indicate that APA mechanisms in S. pombe and S. cerevisiae are largely different: S. pom

60 n, a section on some key differences between S. pombe and S. cerevisiae is included for readers with

61 ighly purified factors between reconstituted S. pombe and S. cerevisiae transcription systems, we con

62 f the transcription mechanism differ between S. pombe and S. cerevisiae, but are conserved between S.

63 ing the distinct initiation patterns between S. pombe and S. cerevisiae, but rather, these patterns a

64 us, whereas m(7)G caps are essential in both S. pombe and S. cerevisiae, m(2,2,7)G caps are not.

65 Therefore the S. pombe and S. japonicus mating systems provide the fir

66 ucleosome-excluding sequences functioning in S. pombe and S. octosporus, and binding sites for trans-

67 nscriptional interference are shared between S. pombe and the highly divergent budding yeast Saccharo

68 r and Tc5 elements by horizontal transfer in S. pombe (and humans) is accompanied by alteration of th

69 pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae.

70 Cdc15, which is essential for cytokinesis in S. pombe, and in the related PCH protein, Imp2.

71 We propose that many hotspots in S. pombe, and perhaps other organisms, result from simpl

72 Human Rab6 can substitute Ryh1 in S. pombe, and therefore Rab6 may be a potential activato

73 cts affect but do not deplete nucleosomes in S. pombe, and they prefer special rotational positions w

74 species approximately 350 million years ago, S. pombe appears to have evolved less rapidly than S. ce

75 sidues involved in binding each substrate of S. pombe Aps1 are unknown.

76 Since S. cerevisiae and S. pombe are evolutionary distant, this methodology will

77 The popularity of S. pombe as a model organism suggests that this augmente

78 ome lies at the heart of the exploitation of S. pombe as a model system.

79 able at http://cerevisiae.oridb.org/ and for S. pombe at http://pombe.oridb.org/.

80 osomes in vivo in Schizosaccharomyces pombe (S. pombe) at base pair resolution.

81 cts the kinase activity and stability of the S. pombe Aurora B homologue, Ark1, colocalizes with know

82 We estimate that dispersal of S. pombe began during human antiquity ( approximately 34

83 gamma-H2A/X phosphate is likely conserved in S. pombe Brc1 and human Mdc1 genome maintenance proteins

84 iated heavy metal detoxification not only in S. pombe but also in some invertebrates while at the sam

85 ng is highly predictable by A/T frequency in S. pombe but not in S. cerevisiae, suggesting that the g

86 rved biological pathways that are present in S. pombe, but not S. cerevisiae, and will enable a compr

87 This Primer introduces S. pombe by describing the yeast itself, providing a bri

88 aneous phenotypic mutations can be mapped in S. pombe by Solexa sequencing.

89 - and Q-independent tRNA methylation site in S. pombe, C34 of tRNA(Pro).

90 ine the three-dimensional arrangement of the S. pombe Ccq1-Tpz1-Poz1 (CTP) complex.

91 us by the FEAR network and Cdk1, whereas the S. pombe CDC14-like phosphatase Clp1p (also known as Flp

92 DCAF2/Cdt2, which is related to S. pombe Cdt2, functions in Xenopus egg extracts and hum

93 ion by competitive binding in both human and S. pombe cells as EGFP fusion proteins.

94 Our work demonstrates that quiescent S. pombe cells assemble specific cytoskeleton structures

95 IR exposure of sporulating S. pombe cells induced misrepair and irreparable DNA dou

96 S. pombe cells lacking tit1+ exhibit slow growth in glyc

97 A damage sites could provide a mechanism for S. pombe cells to arrest at G(2)/M boundary in response

98 S. pombe cells treated with a lethal concentration of av

99 Indeed, Rho1-deficient S. pombe cells were strongly hypersensitive to avicin G,

100 osynthesis is selectively blocked in mutated S. pombe cells, their ability to acquire exogenous hemin

101 ade by mathematical models of endocytosis in S. pombe cells.

102 peraccumulation of ubiquitinated proteins in S. pombe cells.

103 scopic observation of hundreds of individual S. pombe cells.

104 vious studies have suggested that functional S. pombe centromeres lack regularly positioned nucleosom

105 nteractions were captured between and within S. pombe chromosomes.

106 Thus, the impact of S. pombe CLASP on interphase microtubule behavior is mor

107 Third, Peg1 antagonized the action of S. pombe CLIP170 (Tip1) and EB1 (Mal3).

108 proteome-wide binary protein interactome for S. pombe, comprising 2,278 high-quality interactions, of

109 S. pombe contains a single Mis18 isoform that forms a ho

110 logous end joining to be largely faithful in S. pombe, contrary to current belief.

111 Significantly, 3' UTR shortening in S. pombe coordinates with up-regulation of expression fo

112 We isolated multiple S. pombe ctp1 mutants deficient in clipping but proficie

113 nitiator Dpb11 (ortholog of human TopBP1 and S. pombe Cut5), and the multifunctional nuclease/helicas

114 bers, Cdc15 and Imp2, play critical roles in S. pombe cytokinesis.

115 s of tRFs for eight species: R. sphaeroides, S. pombe, D. melanogaster, C. elegans, Xenopus, zebra fi

116 e switch-activating protein Sap1 is a GRF in S. pombe, demonstrating the general applicability of our

117 rticle outlines the way in which interest in S. pombe developed and spread from Europe to Japan, Nort

118 Upon mitotic checkpoint activation, S. pombe Dma1 concentrates at spindle pole bodies (SPBs)

119 S. pombe does not have a well-defined nucleosome-deplete

120 Comparison of the S. pombe E-MAP to an analogous genetic map from the budd

121 Although comparable to S. pombe eMTOCs, A. nidulans sMTOCS are permanent septum

122 ound with mutations affecting the same gene, S. pombe erf2 (sp-erf2), encoding sp-Erf2, a palmitoyltr

123 Here, we demonstrate that S. pombe Est1 was required for the telomere association

124 Our results reveal that the UPR in S. pombe executes RIDD in an intricate interplay between

125 An alanine scan of 11 conserved positions of S. pombe Fcp1 identifies Thr(174), Tyr(237), Thr(243), a

126 have observed the growth characteristics of S. pombe for N=100 cells to determine the growth phenoty

127 the Cdc14-family phosphatase, called Clp1 in S. pombe, from being sequestered and inhibited in the nu

128 e than cells lacking zwf1 We propose that in S. pombe Gcd1 and Idn1 act together to shunt glucose int

129 hese studies reveal that convergent genes in S. pombe generate overlapping transcripts in the G1 phas

130 nts, we identified Cdc48-binding proteins in S. pombe, generating a list of many previously unknown p

131 including approximately 40% of nonessential S. pombe genes.

132 26 heptamer sequence occurs in the wild-type S. pombe genome approximately 300 times, but it has been

133 on for each intergenic region and ORF in the S. pombe genome.

134 gest that Hsp90 plays a specific role in the S. pombe glucose/cAMP pathway.

135 Thus, Git1 is a critical component of the S. pombe glucose/cAMP pathway.

136 Although S. pombe growth is resistant to rapamycin, sla1-Delta ce

137 APA features of higher species, and Pab2 in S. pombe has a different role in APA regulation than its

138 This is the first time that S. pombe has been utilized for isolating and characteriz

139 mbe and S. cerevisiae are largely different: S. pombe has many of the APA features of higher species,

140 major motor involved in ring contraction in S. pombe." Here, we show that most of the differences ob

141 Our findings identified a novel S. pombe histone demethylase with specificity toward di-

142 MT-1 suppresses the Cd2+ hypersensitivity of S. pombe hmt-1 mutants and localizes to the vacuolar mem

143 Crucially, similar analyses of S. pombe hmt-1 mutants extend this finding to show that

144 Genetic ablation of the S. pombe homologs, splsd1 and splsd2, resulted in slow g

145 Msc1 co-precipitates Rhp6, the S. pombe homologue of the human ubiquitin-conjugating en

146 alian XPG (also known as ERCC5) and ERCC1 in S. pombe homologues Rad13 and Swi10 and biochemical inte

147 together with previous observations made for S. pombe homologues tea1p and tea3p, they have broad imp

148 Here we show that binding of the key S. pombe HP1 protein, Swi6, to methylated nucleosomes dr

149 Using the S. pombe HP1 protein, Swi6, we show that recognition of

150 hich is identical to swo1(+) and encodes the S. pombe Hsp90 chaperone protein.

151 thaliana OXS3 to enhance stress tolerance in S. pombe, indicating a role in stress tolerance for the

152 nes are better conserved between the yeasts, S. pombe interactions are significantly better conserved

153 r data argue that trans-histone crosstalk in S. pombe involves direct enhancement of Set1C methyltran

154 orter linker length previously identified in S. pombe is due to a preponderance of nucleosomes separa

155 hat, in contrast to S. cerevisiae, Uap56p in S. pombe is not required for pre-mRNA splicing.

156 Based on studies of S. pombe, it has been proposed that SpHMT-1 transports h

157 In S. pombe, it is known that Rhb1 binds Tor2, and Tor2 inh

158 The S. pombe JmjC family protein Epe1 prevents the ectopic s

159 We show here that the S. pombe JmjC-domain protein Lid2 is a trimethyl H3K4 de

160 ons with the S. japonicus lipin acquiring an S. pombe-like mitotic phosphorylation pattern.

161 Rec10 protein is a major component of S. pombe LinEs and is required for their development.

162 CENP-B homologues of S. pombe localize at and recruit histone deacetylases to

163 ponse, we show that modest overexpression of S. pombe los1(+) (also known as Xpo-t), encoding the nuc

164 subtilis lumazine synthase (Ki 2.6 microM), S. pombe lumazine synthase (Ki 0.16 microM), M. tubercul

165 In S. pombe, many actomyosin ring components assemble at th

166 These results suggest that PDE activity in S. pombe may be coordinately regulated with adenylate cy

167 A point mutant within this motif of S. pombe Mcm10p was defective in primer synthesis in vit

168 In contrast to S. pombe, medial assembly of the actomyosin ring in mito

169 cuoles and abrogates (35)S-PC(2) uptake into S. pombe microsomal vesicles.

170 at Mal3 makes a distinctive footprint on the S. pombe microtubule lattice and that unlike mammalian m

171 tice and that unlike mammalian microtubules, S. pombe microtubules do not show the longitudinal latti

172 stituted dynamically unstable single isoform S. pombe microtubules with full length Alp14/TOG and Alp

173 and functional analysis of human anillin and S. pombe Mid1.

174 is I and to suppress merotelic attachment in S. pombe mitosis, and crosslinking rDNA repeats to aid r

175 of Rab-GTPase activity is a property of the S. pombe MOP essential for the initiation of membrane fo

176 providing the first identification of these S. pombe mtDNA discrepancies.

177 sufficient for polar cell extension, but in S. pombe, MTs are in addition required for the establish

178 n approach to directly select for long-lived S. pombe mutants from a random DNA insertion library.

179 S. pombe mutants lacking a new factor described here, Er

180 Interestingly, proteasome-defective S. pombe mutants were not markedly hypersensitive to avi

181 In vitro studies of S. pombe MYH identified residues I261 and E262 of the ID

182 Here, we show that the sole S. pombe myosin I, myo1p, is required for proper organiz

183 E1-Sup1 cells depend on the late cytokinetic S. pombe myosin II isoform, Myp2p, a non-essential prote

184 e localization to nuclei and mitochondria in S. pombe, neither of the S. cerevisiae homologs, nor hum

185 rs of low oxygen adaptation, we screened the S. pombe nonessential haploid deletion collection and id

186 ns of the chromosome organization within the S. pombe nuclei were made by polymer modeling.

187 In the fission yeast S. pombe, nuclei are actively positioned at the cell cen

188 revisiae, A/T-rich sequences are enriched in S. pombe nucleosomes, particularly at +/-20 bp around th

189 We conclude that the S. pombe nucleus is spatially divided into functional su

190 t levels have preferred positions within the S. pombe nucleus.

191 Because all well characterized S. pombe origins are located in intergenic regions, we a

192 formation of stable protein-DNA complexes at S. pombe origins of replication involves binary interact

193 We show that the S. pombe ortholog Seb1 is associated with pericentromeri

194 Oxs1 and Pap1-homologues can substitute for S. pombe Oxs1 and Pap1 to enhance stress tolerance.

195 f transcriptional interference involving the S. pombe pho1(+) gene.

196 ogether, results reported here revealed that S. pombe possesses an unexpected pathway for heme assimi

197 Studies of S. pombe Pot1-DBD and its individual OB-fold domains rev

198 domain (residues 1-187) found in full-length S. pombe Pot1.

199 Two crystal structures of S. pombe profilin and homology models of S. pombe profil

200 of S. pombe profilin and homology models of S. pombe profilin bound to actin show how the two profil

201 Human profilin-I and S. pombe profilin have similar affinities for actin mono

202 in filament elongation by formin Cdc12p like S. pombe profilin.

203 an bind and unwind both DNA and RNA, but the S. pombe protein is not essential and has not been demon

204 The essential S. pombe protein Teb1 contains two Myb-like DNA binding

205 cterized fungal proteins, including a second S. pombe protein that is not functionally redundant with

206 ere, we isolate a previously uncharacterized S. pombe protein through association with the Cdc14 phos

207 at one of the AT-STUbLs least related to the S. pombe protein, AT-STUbL4, has acquired a plant-specif

208 occurs in the 5' to 3' direction, as for the S. pombe protein.

209 n of this new technology for the analysis of S. pombe proteins.

210 minished for Atl1 R69A and R69F mutants, and S. pombe R69A and R69F mutants are more sensitive toward

211 verified utility by C-terminally tagging the S. pombe rad4 and swi1 genes with yEGFP and the yEGFP de

212 e been able to identify homologous motifs in S. pombe Rad9 that can activate Mec1.

213 When the mammalian homologue of S. pombe Rad9 was inactivated, increases in chromosome e

214 zfs1 and its targets in S. pombe represent a useful model system for studies of

215 However, we find that S. pombe requires cap guanine-N7 methylation catalyzed b

216 dition to the endoribonuclease Dcr1, RNAi in S. pombe requires two interacting protein complexes, the

217 We describe the expansion of S. pombe research during this period with an emphasis on

218 emperature-sensitive and knockout strains of S. pombe, respectively, further verified the functions o

219 than deleting their singleton counterpart in S. pombe, revealing post-duplication adaptation.

220 ns identified here as subject to skipping in S. pombe reveals high sequence conservation and perfect

221 isome component C20orf43/RTF2 (homologous to S. pombe Rtf2) must be removed for fork restart to be op

222 s ability to insert within silent regions of S. pombe's genome.

223 genome-scale cell-cycle mRNA expression from S. pombe, S. cerevisiae and human.

224 Purification of the S. pombe SAGA complex showed that its subunit compositio

225 Analysis of S. pombe SAGA mutants revealed that SAGA has two opposin

226 , Pidoux et al. and Williams et al. identify S. pombe Scm3 as the proximate factor in the Cnp1/CENP-A

227 romeric nucleosomes, the dynamic behavior of S. pombe Scm3 suggests that it acts as a Cnp1 assembly/m

228 factors, deletion of the SpELL gene renders S. pombe sensitive to the drug 6-azauracil.

229 To ask if the S. pombe septins function redundantly in cytokinesis, we

230 Taken together, our results suggest that the S. pombe septins participate redundantly in one or more

231 We found that the activity of S. pombe Set1C toward nucleosomal histone H3 is directly

232 d Spt8 have opposing regulatory roles during S. pombe sexual differentiation.

233 Global analysis of tRNA methylation in S. pombe showed a striking selectivity of Pmt1 for tRNA(

234 s in each species were found to display APA, S. pombe showed greater 3' UTR size differences among AP

235 lease expression in cells harbouring a novel S. pombe single-strand annealing (SSA) assay.

236 ith single-particle averaging to localize 14 S. pombe SPB components and regulators, determining both

237 e first comprehensive molecular model of the S. pombe SPB, resulting in structural and functional ins

238 mental role for Ppc89 in organization of the S. pombe SPB.

239 ich is highly diverged from the well-studied S. pombe species (with 44% GC content).

240 S. japonicus differ vastly from those of the S. pombe species.

241 Here we report that deletion of the S. pombe Spt5 CTD results in slow growth and aberrant ce

242 Thus, the S. pombe STE, although distant from the template, ensure

243 The laboratory strain and most other S. pombe strains contain three chromosomes, but one rece

244 Using S. pombe strains deficient for Pcf11 or Pab2, we show th

245 ed mmd4 in a screen of temperature-sensitive S. pombe strains for aberrant mitochondrial morphology a

246 SVs in the genomes of a worldwide library of S. pombe strains, including duplications, deletions, inv

247 cript levels in wild-type and zfs1-deficient S. pombe strains; those elevated in the zfs1-deficient s

248 The S. pombe stress response pathway (SRP) also promotes com

249 In S. pombe, strong motifs surrounding distal PASs lead to

250 een S. pombe and humans, suggesting that the S. pombe structure may be a good surrogate for that of t

251 Functions for RNAi revealed in S. pombe, such as heterochromatic silencing and chromoso

252 s observed in B. subtilis, S. cerevisiae and S. pombe, such as the tendency of FRS to increase from t

253 Recent data from S. pombe suggest an alternative possibility: that the re

254 In S. pombe, Swi1 and Swi3 form the replication fork protec

255 ntriguingly, unlike their human counterpart, S. pombe SWIRM complex contains neither a histone deacet

256 Thus, the S. pombe system can be used to identify novel, evolution

257 ken together, these results suggest that the S. pombe system described here can be employed for compa

258 ctions that are important in maintaining the S. pombe telomere in a non-extendible state.

259 eotide consisting of two conserved hexameric S. pombe telomere repeats, d(GGTTACGGTTAC), with an affi

260 The identified Tbf1p binds S. pombe telomeric DNA with high sequence specificity in

261 We further show that the S. pombe telomeric protein Tpz1, like its mammalian homo

262 the heterogeneous spacers that occur between S. pombe telomeric repeats, and it also has implications

263 While the S. pombe TER intron contains the canonical 5'-splice sit

264 d by Tgs2 can be converted to m(2,2,7)GDP by S. pombe Tgs1 in the presence of excess AdoMet.

265 A genetic analysis of S. pombe Tgs1 showed that it is nonessential.

266 An S. pombe tgs1Delta strain grows normally, notwithstandin

267 is a TOG-family microtubule polymerase from S. pombe that tracks plus ends and accelerates their gro

268 ify a pause in early elongation, specific to S. pombe, that requires the conserved elongation factor

269 at for two distant yeasts (S. cerevisiae and S. pombe), the only other organisms comprehensively exam

270 In S. pombe, the ER membrane-resident kinase/endoribonuclea

271 Remarkably however, similar to S. pombe, the S. japonicus cells switch cell/mating type

272 In S. pombe, the SIN and Clp1 act as part of a cytokinesis

273 hat sla1(+) regulates AAM mRNA production in S. pombe through its effects on nuclear tRNA processing

274 tch from proliferation to differentiation in S. pombe through the dynamic and opposing activities of

275 s scenario may principally be conserved from S. pombe to filamentous fungi.

276 shu1(+) that encodes a protein that enables S. pombe to take up extracellular heme for cell growth.

277 Studies of S. pombe, together with studies of its distant cousin, S

278 hat Rhb1 interacts with Tor2, one of the two S. pombe TOR (Target of Rapamycin) proteins.

279 A screen with S. pombe transcription factor mutant strains for growth

280 phy reveals that microtubules assembled from S. pombe tubulin have predominantly B-lattice interproto

281 Unlike S. pombe, two other fission yeasts rely on hyperstabiliz

282 leavage mechanism is more ancestral than the S. pombe-type.

283 s could completely substitute for the native S. pombe TZF domain, as determined by measurement of tar

284 Here, we report the structure of S. pombe Uba1 in complex with Ubc15, a Ub E2 with intrin

285 of protein SUMOylation, and we identified an S. pombe Ulp2/Smt4 homolog that, when overexpressed, red

286 -specific transcriptome of the fission yeast S. pombe under multiple growth conditions using a novel

287 The cassette consists of the S. pombe ura4(+) selectable marker flanked by a wild-typ

288 Thus, S. pombe uses a universally conserved stress-sensing mac

289 reas both species divide in the middle, only S. pombe uses the anillin Mid1 as a primary nucleus-deri

290 SINaTRA by predicting synthetic lethality in S. pombe using S. cerevisiae data, then identify over on

291 ters abolishes phytochelatin accumulation in S. pombe vacuoles and abrogates (35)S-PC(2) uptake into

292 are lethal in S. cerevisiae, they are not in S. pombe We show that the lethality of a temperature-sen

293 rgosterol with respect to wild-type Dap1p in S. pombe, we find that Dap1pY138F expression is still su

294 a genome-wide approach in the fission yeast S. pombe, we have found that Dcr1, but not other compone

295 nes suggests Tf1 may improve the survival of S. pombe when cells are exposed to environmental stress.

296 port a novel mechanism for oxygen-sensing in S. pombe, whereby the 2-OG-Fe(II) dioxygenase Ofd protei

297 Our results suggest that S. pombe will be a useful model organism for elucidating

298 mouse, rat, mouse-ear cress, fruit fly, the S. pombe yeast, the E. coli bacterium and the M. jannasc

299 When expressed in S. pombe, YopJ sensitized cells to osmotic and oxidative

300 in techniques, we replaced the TZF domain of S. pombe Zfs1 with the equivalent domains from human TTP