ライフサイエンスコーパス: S1 nuclease

1 dimethylsulphate, potassium permanganate and S1 nuclease.

2 at 37 degree C, although it is hydrolyzed by S1 nuclease.

3 digesting the resulting RNA-DNA hybrids with S1 nuclease.

4 een mapped within the OAX HindIII unit using S1 nuclease.

5 ammalian serum and to endonucleases, such as S1 nuclease.

6 if the molecules are previously treated with S1 nuclease.

7 reaction and made this fragment sensitive to S1 nuclease activity.

8 Primer extension and S1 nuclease analyses identified prominent (+1/+2) and mi

9 Reverse transcriptase-PCR and S1 nuclease analyses revealed that this unique ORF is tr

10 Primer extension and S1 nuclease analyses showed the presence of three transc

11 S1 nuclease analysis and immunoblotting showed that the

12 S1 nuclease analysis demonstrated intact full-length AS-

13 Primer extension and S1 nuclease analysis identified a sigma54 promoter upstr

14 Primer extension and S1 nuclease analysis identified two transcription initia

15 Primer extension and S1 nuclease analysis of C. cellulovorans RNA showed that

16 Differences in the mRNAs were confirmed by S1 nuclease analysis of placental RNA with a cDNA probe

17 S1 nuclease analysis reveals a single region of hypersen

18 for cvaA identified by primer extension and S1 nuclease analysis, P1, lies 320 bp upstream of the tr

19 Using S1 nuclease analysis, we determined that the distance be

20 criptase PCR (RT-PCR), primer extension, and S1 nuclease analysis.

21 Similarly, both S1 nuclease and 2D gel electrophoresis of DNA topoisomer

22 By using S1 nuclease and 5'- rapid amplification of cDNA ends, th

23 ility against snake venom phosphodiesterase, S1 nuclease and in fetal calf serum.

24 This region is sensitive to S1 nuclease and likely to assume a non-B-form DNA second

25 ay DNA supercoiling-dependent sensitivity to S1 nuclease and OsO4, which is consistent with a non-B-D

26 ve c-myc genes yielded different patterns of S1 nuclease and permanganate sensitivity, indicating alt

27 talurid herpesvirus 1) genome were mapped by S1 nuclease and primer extension analyses as well as by

28 mparison of the prtF and rofA transcripts by S1 nuclease and primer extension assays indicated that t

29 orter plasmids using confocal Raman spectra, S1 nuclease and restriction enzymes demonstrated that th

30 S1 nuclease and RNase protection assays identified two c

31 S1 nuclease and RNase protection assays revealed the pre

32 rse transcriptase polymerase chain reaction, S1 nuclease, and functional cell surface binding studies

33 in all major brain regions as determined by S1 nuclease assay and in a variety of specific neuroanat

34 S1 nuclease assay confirmed the ligand mediated G-quadru

35 The S1 nuclease assay showed that the accelerated mean TRF l

36 l restriction fragment (TRF) length, and the S1 nuclease assay was used to determine the frequency of

37 a defined sequence of DNA, as verified using S1 nuclease cleavage assays.

38 airpin helices, is indicated by preferential S1 nuclease cleavage at the center of the oligomer(s), i

39 findings were consistent with the results of S1 nuclease cleavage observed at B-Z junctions flanking

40 lesions, as measured by quantitative PCR and S1 nuclease cleavage of single-strand break sites.

41 22 monoclonal antibody (ZIBS assay); (ii) an S1 nuclease cleavage-primer extension assay to map B-Z j

42 t binds cruciform-specific 2D3 antibody, and S1 nuclease-cleavage and OsO4-modification assays have i

43 Using Southern blotting of S1 nuclease-digested FIV preintegration complexes isolat

44 and APAF anti-DNA is much more sensitive to S1 nuclease digestion of denatured dsDNA.

45 Reverse transcriptase PCR and S1 nuclease digestion were used to compare mRNA levels d

46 A strategy integrating S1 nuclease digestion with SERS detection was developed

47 r nondenaturing conditions, was sensitive to S1 nuclease digestion, and hybridized to only one of two

48 tpB mRNA from PS+ genome-containing cells to S1 nuclease digestion.

49 Analysis by topoisomerase assays, S1 nuclease digests, and atomic force microscopy showed

50 rt a novel whole-genome method, S1-DRIP-seq (S1 nuclease DNA:RNA immunoprecipitation with deep sequen

51 ally amplifying conjugated polymer (CP), and S1 nuclease enzyme is capable of detecting SNPs in a sim

52 Quantitative S1 nuclease experiments showed that expression of the fi

53 S1 nuclease experiments showed that level of the greA tr

54 mspA gene was mapped by primer extension and S1 nuclease experiments.

55 An S1 nuclease-hypersensitive region (5'SHS) was identified

56 uch transcriptional control elements, the 5'-S1 nuclease-hypersensitive silencer (5'SHS; -1418 to -13

57 ich strand of a PDGF-A silencer sequence (5'-S1 nuclease-hypersensitive site (SHS)) yielded three cDN

58 These sites are also sensitive to S1 nuclease in erythroid cells in vivo, suggesting a dis

59 Low resolution analysis with S1 nuclease indicates that DNA in this region was unpair

60 S1 nuclease is shown to cleave the displaced strand of t

61 ption initiation site was determined by both S1 nuclease mapping and 5' rapid amplification of cDNA e

62 S1 nuclease mapping and primer extension demonstrated th

63 site of the primary transcript was mapped by S1 nuclease mapping and the major promoter was defined b

64 S1 nuclease mapping and Western blot analyses demonstrat

65 S1 nuclease mapping assays show that the truncated trans

66 ded by a TATA box in the usual position, and S1 nuclease mapping at each exon 1 revealed multiple tra

67 S1 nuclease mapping confirmed that transcription of both

68 in these cells were analyzed by quantitative S1 nuclease mapping for nuclear accumulation, intron exc

69 Primer extension and S1 nuclease mapping identified three transcription start

70 S1 nuclease mapping indicates that rpoH transcripts orig

71 S1 nuclease mapping of the trpD region revealed four fra

72 S1 nuclease mapping shows that the primary terminator is

73 S1 nuclease mapping shows that this region contains the

74 S1 nuclease mapping studies of trpCXBA have revealed two

75 transcription is feedback regulated, whereas S1 nuclease mapping suggests that emp2 mutant transcript

76 er, our data from primer extension analysis, S1 nuclease mapping, beta-galactosidase assays, and in v

77 Using S1 nuclease mapping, lacZ transcriptional fusions, and i

78 Using mutational analysis, S1 nuclease mapping, quantitative RT-PCR, and transcript

79 merase chain reaction, primer extension, and S1 nuclease mapping, were located approximately 650 base

80 identified by primer extension analysis and S1 nuclease mapping.

81 f mLAL were characterized by 5'-RACE-PCR and S1 nuclease mapping.

82 The S1-nuclease mapping and primer extension analysis reveal

83 S1 nuclease mappings confirmed further the induction eff

84 sive to cleavage with single-strand-specific S1 nuclease or the single-strand-selective agent potassi

85 Functional analysis of mutant aptamers, S1 nuclease probing, and comparative sequence analysis i

86 rvation was examined by primer extension and S1 nuclease protection analyses of genes encoding enzyme

87 ATG initiation codon by primer extension and S1 nuclease protection analyses of RNA from human skin a

88 A comparison of ribonuclease and S1 nuclease protection analyses on native TGF-beta 1 mRN

89 ipts were determined by primer extension and S1 nuclease protection analyses respectively.

90 Primer extension and S1 nuclease protection analyses were used to determine t

91 S1 nuclease protection analysis showed that transcriptio

92 S1 nuclease protection analysis with human liver, prosta

93 Using S1 nuclease protection analysis, we mapped the katG mRNA

94 d corneal endothelial cells were analyzed by S1 nuclease protection analysis, whereas the nucleotide

95 and the 5' ends of ahpC mRNA were mapped by S1 nuclease protection analysis.

96 RhEBV-transformed cell lines was detected by S1 nuclease protection analysis.

97 S1 nuclease protection and enzyme-linked immunosorbent a

98 ble during in vitro growth, as determined by S1 nuclease protection and primer extension analyses of

99 aI transcript start site, which we mapped by S1 nuclease protection and primer extension analyses.

100 We used S1 nuclease protection and primer extension assays to de

101 However, S1 nuclease protection and primer extension experiments

102 stigated in M. smegmatis and M. bovis BCG by S1 nuclease protection and transcriptional fusion analys

103 An S1 nuclease protection assay and a Western blot analysis

104 Both S1 nuclease protection assay and primer extension study

105 An S1 nuclease protection assay demonstrated that AlgR repr

106 An S1 nuclease protection assay showed that the start of th

107 A quantitative S1 nuclease protection assay was developed to allow comp

108 By 5'-rapid amplification of cDNA ends and S1 nuclease protection assay, we determined that the tra

109 ncrease in TPO mRNA levels as measured by an S1 nuclease protection assay.

110 Quantitative S1 nuclease protection assays and a promoterless catecho

111 RACE (rapid amplification of cDNA ends) and S1 nuclease protection assays and was at the site of an

112 S1 nuclease protection assays corroborated these finding

113 apid amplification of cDNA ends coupled with S1 nuclease protection assays demonstrate that the M3 pr

114 transcripts by primer extension and modified S1 nuclease protection assays demonstrated that transcri

115 S1 nuclease protection assays indicated that this 34-bp

116 S1 nuclease protection assays of rpoH P1- and P2-specifi

117 S1 nuclease protection assays revealed that four ftsZ tr

118 Furthermore, S1 nuclease protection assays show that Cibacron blue ca

119 S1 nuclease protection assays using a probe which hybrid

120 S1 nuclease protection assays were used to determine tra

121 Primer extension and S1 nuclease protection assays were used to identify two

122 and metabolism of btuB RNA were analyzed by S1 nuclease protection assays, and mutations that alter

123 examined by a variety of methods, including S1 nuclease protection assays, Northern blotting, Wester

124 rse transcription-PCR analyses, coupled with S1 nuclease protection assays, provided evidence that ge

125 ns (NTRs) were assayed for RNA production by S1 nuclease protection assays.

126 n levels using reverse transcription-PCR and S1 nuclease protection assays.

127 e 5' rapid amplification of cDNA end-PCR and S1 nuclease protection assays.

128 Primer extension analysis and S1 nuclease protection experiments identified the 5' end

129 S1 nuclease protection experiments indicated that induce

130 We now show by S1 nuclease protection experiments that a typical shift-

131 This is not apparent if S1 nuclease protection is used alone, which emphasizes t

132 Primer extension of the 5' UTRs and S1 nuclease protection of the 3' UTRs initially identifi

133 5-HT2C-R transcript, first identified using S1 nuclease protection of total RNA isolated from the ch

134 ed both rapid amplification of cDNA ends and S1 nuclease protection protocols and examined the organi

135 S1 nuclease protection studies showed that increased tra

136 Based on strand-specific RT-PCR, S1 nuclease protection, and RNA gel blots, evidence was

137 E-selectin mRNA, analyzed by S1 nuclease protection, consists of a single predominant

138 tely initiated rRNA transcripts, detected by S1 nuclease protection, were paralleled by increased lev

139 apped within the third intron by 5' RACE and S1 nuclease protection.

140 ese fragments are generated from much longer S1-nuclease sensitive fragments of foreign DNA that requ

141 eric junction sequence possesses an unusual, S1 nuclease-sensitive conformation (anisomorphic DNA), w

142 Fine mapping of S1 nuclease-sensitive sites suggests that the PPY/PPU tr

143 he DNA was examined under native conditions, S1 nuclease-sensitive ssDNA was identified in all strain

144 It did not cause this same S1 nuclease sensitivity in the remainder of pRQ7.

145 These elements confer S1 nuclease sensitivity on isolated plasmid DNA at low p

146 This fragment conferred S1 nuclease sensitivity on the resulting supercoiled pla

147 n of the non-B DNA structure responsible for S1 nuclease sensitivity upstream of the MUC1 gene.

148 dopt ss DNA conformation, as demonstrated by S1 nuclease sensitivity.

149 A combination of primer extension and S1 nuclease studies identifies two transcriptional start

150 After subsequent treatment with S1 nuclease, the cationic water soluble CP electrostatic

151 rophoretic mobility shift assays (EMSAs) and S1 nuclease treatment are used to demonstrate that the R

152 specificity of APAF and APAD was assayed by S1 nuclease treatment of heat-denatured DNA.

153 mal APAF and APAD was much more sensitive to S1 nuclease treatment than similar fractions from SLE pa

154 iumhexylbromide)fluorene)-co-phenylen e] and S1 nuclease, unambiguous FRET signaling is achieved for

155 equent footprinting studies with DNase I and S1 nucleases using a supercoiled plasmid DNA containing