ライフサイエンスコーパス: cistron

1 while allowing translation of the downstream cistron.

2 ing translation of the associated downstream cistron.

3 extended to fusion junctions within each alc cistron.

4 activation of translation of the resistance cistron.

5 6 to transactivate expression of an internal cistron.

6 that represses translation of the downstream cistron.

7 ysis of the bacteriophage Q beta readthrough cistron.

8 447 nucleotides from within the readthrough cistron.

9 es the access of ribosomes to the downstream cistron.

10 llow efficient translation of the downstream cistron.

11 ing frame located upstream of the resistance cistron.

12 cistron; and (c) a group I intron in the 43B cistron.

13 s ruled out possible effects of the reporter cistron.

14 omplex during translation of the ermC leader cistron.

15 Gtx or FGF2 5' leaders linked to a reporter cistron.

16 that contains the mir-17-92 microRNA (miRNA) cistron.

17 the ability to support translation of the 3' cistron.

18 t encoded in a different frame within the P3 cistron.

19 16 (VP16) transcription factor as the second cistron.

20 me in which NS2-3 was restored in the second cistron.

21 dependent of the translation of the upstream cistron.

22 if positioned 134 nt upstream of the distal cistron.

23 ach His-tagged and in separate transcription cistrons.

24 usability in the forward design of synthetic cistrons.

25 inserted between the upstream and downstream cistrons.

26 er and that are uncapped or contain multiple cistrons.

27 for polymorphism in the P1, P3, NIa, and CP cistrons.

28 nimal sequence difference was detected among cistrons.

29 mRNA stimulated expression of the downstream cistron 42-fold.

30 TS) that splits the polymerase gene into two cistrons, 43A and 43B, corresponding to N-terminal (gp43

31 ort open reading frame, termed the 5' leader cistron (5'LC), which is 102 bp upstream of the beta2AR

32 the translational operator of the replicase cistron, a 19 nt fragment (TR).

33 ronic mRNAs with the HIS3 gene as the second cistron and 18 random nucleotides in the intercistronic

34 a monocistronic mRNA encoding the downstream cistron and false identification of an IRES.

35 ctor, which has Renilla luciferase as the 5' cistron and firefly luciferase as the 3' cistron, has be

36 microsomal P450 enzyme encoded by the first cistron and the auxiliary protein NADPH-P450 reductase b

37 Renilla luciferase or the EGFP) as the first cistron and the yeast Gal4/viral protein 16 (VP16) trans

38 ge genetic engineering projects require more cistrons and consequently more strong and reliable trans

39 HEG) inserted between the IC-UTS and the 43B cistron; and (c) a group I intron in the 43B cistron.

40 hin the intergenic spacer region of the rDNA cistron, approximately 3 kb upstream (5') of the 18S gen

41 bryophyte) chloroplasts, where the first two cistrons are separated by a spacer region to which no si

42 n by WSMV-GFP, suggesting that both of these cistrons are SIE effectors encoded by WSMV.

43 .4 UTR efficiently translated the luciferase cistron as well, demonstrating the presence of an intern

44 e hairpin, inhibits translation of the first cistron but does not inhibit translation of the cistron

45 ORF2) inhibits translation of the downstream cistron by a process that depends on the uORF2 amino aci

46 and translational control strategies for the cistrons contained therein, but with different specifici

47 hus, ribosomes translating the upstream pabB cistron could be capable of reducing TRAP-dependent cont

48 ions that abolished translation of the first cistron did not, however, affect the IRES-mediated trans

49 tron but does not inhibit translation of the cistron downstream of the c-jun 5' UTR.

50 The downstream cistron encoded a reporter protein (chloramphenicol acet

51 icistronic transcripts in which the upstream cistron encoded PTB or PTB deletion mutants (including a

52 The first cistron encodes the well-characterized alpha1A subunit.

53 cap-independent translation of a downstream cistron encoding Firefly luciferase in a dicistronic exp

54 lthough at least one change occurred in each cistron except HC-Pro and P3.

55 ing events that could account for downstream cistron expression.

56 s conserved despite the duplication of the H cistron for both vectors.

57 gh CAT activity was expressed from the first cistron from all of the dicistronic constructs introduce

58 strategy used by viruses to express several cistrons from one mRNA.

59 rying an insertion-deletion mutation in this cistron germinated normally; thus, the role of GrmA in s

60 5' cistron and firefly luciferase as the 3' cistron, has been found to generate spliced transcripts.

61 88-nt 5'-terminal segment of the 738-nt nsp1 cistron in a BCoV defective interfering (DI) RNA.

62 136 CDS supported expression of a downstream cistron in a bicistronic reporter system.

63 that mediates the translation of the second cistron in a dicistronic mRNA in cultured mammalian cell

64 er could enhance the translation of a second cistron in a dicistronic mRNA.

65 oth M2 and M3 were expressed from a separate cistron in a VSV mutant background that readily establis

66 t the efficient translation of a second URA3 cistron in dicistronic mRNAs in S. cerevisiae, thereby c

67 that nsp4 to nsp10/11 functions as a single cistron in negative-strand RNA synthesis and analyze rec

68 smic acid by enzymes encoded in a seven-gene cistron in P. aeruginosa and in other Pseudomonads.

69 zed steps by enzymes encoded in a seven-gene cistron in Pseudomonas and other organisms.

70 These results identify a new cistron in the MHV replicase gene locus and show that ns

71 translated only the upstream chloramphenicol cistron in transiently transfected mammalian cells.

72 nd polyamines on translation of a downstream cistron in vivo and support the hypothesis that polyamin

73 ses translation of the associated downstream cistron in vivo.

74 nscript cleavage with individually processed cistrons in operons and gene expression regulated by nuc

75 Our results demonstrate that the downstream cistron, in the bicistronic gene, is expressed to a much

76 Expression of this internal cistron, in the presence of P6, is greatly enhanced by t

77 the IRES-mediated translation of the second cistron, indicating that this IRES-mediated translation

78 ediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling

79 15q11-q13 and is paternally expressed, each cistron is a candidate for a role in the imprinted Prade

80 ains indicates that expression of the second cistron is spatially and temporally regulated.

81 ing increase in the translation of the first cistron (luciferase or EGFP) is monitored either by meas

82 activation of the Qbeta coliphage maturation cistron, mediated by the presence of Qbeta replicase.

83 is not due to readthrough from the upstream cistron, nor is it due to translation of cryptic monocis

84 osome entry site, expression from the distal cistron of a dicistronic mRNA increased as a function of

85 The E.gracilis rpoA gene is the distal cistron of a multigene cluster that includes genes for c

86 ast, the efficient translation of the second cistron of bicistronic mRNAs, directed by two distinct I

87 report an initial characterization of the E1 cistron of HPV type 16 (HPV-16), the most common oncogen

88 nalyzing ribosome stalling at the regulatory cistron of the antibiotic resistance gene ermA, we uncov

89 beta(2)AR upstream peptide of the 5'-leader cistron of the beta(2)AR, and this polymorphism in the b

90 nting protein is translated from a different cistron of the same RNA genome; (iii) 3AB is the most li

91 enzyme SP lyase encoded by splB, the second cistron of the splAB operon.

92 on and point mutations was created in the CP cistron of wild-type and/or green fluorescent protein-ta

93 , the presence of the 5'-UTR between the two cistrons of a bicistronic mRNA stimulated expression of

94 but not all copies of the nuclear ribosomal cistrons of an individual organism, and the changing rat

95 The P1, HC-Pro, P3, CI, NIa, NIb, and CP cistrons of LDSI-S10 and each lineage at passages 1, 3,

96 ent-peptide-dependent stalling at the leader cistrons of several inducible antibiotic resistance gene

97 Transcription from cistrons of the Escherichia coli CytR regulon is activat

98 the uORF represses translation of the HER-2 cistron or of a heterologous reporter gene.

99 1, HC-Pro, P3, 6K1, CI, 6K2, NIa-VPg, or NIb cistrons permitted efficient superinfection by WSMV expr

100 e, petB(fbcB) was split genetically into two cistrons, petB6 and petBIV, which encoded two polypeptid

101 overlap, out of frame, the downstream major cistron, polyamine regulation was abolished.

102 us is made up of two divergently transcribed cistrons, pspF and pspABCDycjXF.

103 ibed spacer region (ITS) of the nuclear rDNA cistron represents the barcoding locus for Fungi.

104 fluorescent proteins as the first and second cistrons, respectively.

105 ection assays, siRNA knockdown of individual cistrons, RT-PCR to detect mRNA encoded by the bicistron

106 We constructed a three-cistron Sindbis virus that expresses the alpha and beta

107 TR between an RNA hairpin and the luciferase cistron stimulated expression 119-fold.

108 the IRES-mediated translation of downstream cistron, suggesting that the IRES activity requires the

109 ic region increased expression of the second cistron, suggesting that the viral sequence can function

110 etic relatives, gene 43 consists of a single cistron that encodes a PolB family (PolB-type) DNA polym

111 uciferase activity generated from the second cistron that was either equivalent or higher than that o

112 (TriMV) encode two independently functioning cistrons that serve as effectors of SIE at the protein b

113 We analyzed the complete nuclear ribosomal cistron, the complete chloroplast genome, a partial mito

114 Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region ha

115 e spacer regions of the ribosomal DNA (rDNA) cistron, to test the hypothesis of unequal mutation rate

116 ed over 100-fold-higher levels of downstream cistron translation than did the Renilla open reading fr

117 ative IRESs promote expression of downstream cistrons via splicing rather than internal initiation of

118 Expression of the second cistron was dependent upon the intercistronic sequences

119 eLa cells, the translation of the downstream cistron was increased by 50-fold, demonstrating that tra

120 The second cistron was not expressed in control dicistronic constru

121 , with c-myc 5' UTR inserted between the two cistrons, was transfected into both HepG2 and HeLa cells

122 of this stop codon, coding for a second mini-cistron, we could not identify another start codon for g

123 hree subunits from the nuclear ribosomal RNA cistron were compared together with regions of three rep

124 ognition sites mapped in the lake trout rDNA cistron were used to digest genomic DNA into fragments o

125 Cells expressing both cistrons were sorted, and sequences recovered from selec

126 the 5.8S gene of the nuclear ribosomal gene cistrons) were aligned, guided by ITS transcript seconda

127 beta-galactosidase (betaGAL) from the second cistron whereas little or no betaGAL was expressed in th

128 assay eliminated translation of the upstream cistron while allowing translation of the downstream cis

129 expression of NS5A alone from an additional cistron within a replicon construct gave greater rescue

130 major differences in the length of the rDNA cistron within individual lake trout, minimal sequence d

131 5A was expressed from an additional upstream cistron within the RNA to be rescued.