ライフサイエンスコーパス: coculture

1 significantly lower in L. rostrata cells in coculture.

2 ng IFN-gamma production by CD4(+) T cells in coculture.

3 2-agonists was greatly reduced in HLMC-HASMC coculture.

4 ), NFkappaB, and SMAD3 in both cell lines in coculture.

5 ffector T-cell proliferation by Treg cell in coculture.

6 cells (HCT116, HT29) grown in monoculture or coculture.

7 r wild-type parents and compete with them in coculture.

8 c oxide (NO) synthesis triggered by P. morii coculture.

9 chromosomal Hg(R) in P. putida was slowed in coculture.

10 death through conditioned medium (CM) and in coculture.

11 lated from an environmental sample by amoeba coculture.

12 uting the specimen in broth and by Vero cell coculture.

13 is upregulated in adipocytes and AML when in coculture.

14 , DCs and autologous naive CD4+ T cells were cocultured.

15 in cell-to-cell spread in MDDC-CD4(+) T cell cocultures.

16 and LX2 cells, using a transwell to generate cocultures.

17 ure under supportive or nonsupportive stroma cocultures.

18 detected in motoneuron-astrocyte noncontact cocultures.

19 f a rho kinase inhibitor and in 2D monolayer cocultures.

20 ) cell differentiation and cTFH/naive B-cell cocultures.

21 evidence of prominent occludin expression in cocultures.

22 ML, and that IL8 was increased in AML/BM-MSC cocultures.

23 surface, enabling IgE-IC uptake by moDCs in cocultures.

24 hat LacNAc suppressed basophil activation in cocultures.

25 After 2 d of coculture, 11% of the eosinophils gained CD16 expression

26 hocyte antigen-positive T cells were used in cocultures, a TH17-dominant response was observed, as re

27 croarchitecture, high-throughput capability, coculture ability, and low risk of cross-contamination.

28 AD1 in metastatic cells by primary glia cell coculture abolished the capacity of metastatic cells to

29 like axonal profiles were established in our cocultures allowing an analysis of putative PNS/CNS axon

30 istamine release was increased in HLMC-HASMC coculture and this was enhanced by beta2-AR agonists.

31 Using neuronal cocultures and isolated brain mitochondria of alpha-, be

32 Typical approaches that rely on bulk cocultures and population-wide correlations, however, on

33 in both HLMCs and HASMCs when the cells were cocultured, and was inhibited by neutralizing SCF or CAD

34 ion or administration of specific factors or coculture approaches with other tissues.

35 ives were evaluated in our three-dimensional coculture assay for the effects of mammary fibroblasts o

36 The conventional Treg cell function coculture assay is however time-consuming and labor inte

37 Using an in vitro coculture assay of H. polygyrus bakeri larvae and bone m

38 Using both in vitro coculture assays and a rat model of adoptive transfer af

39 RCC cells supported EC survival in coculture assays and induced angiogenesis in CAM assays.

40 In cell coculture assays, ICOVIR-15K-cBiTE-mediated oncolysis re

41 Analogous experiments cocultured basophils (1-72 h) directly with EC lines.

42 Coculture between Lg-hDCs and T lymphocytes obtained fro

43 We also set up cocultures between a conditioned medium of treated kerat

44 o formed in the fibrin-Matrigel mixed gel by coculturing brain microvascular endothelial cells (BMECs

45 sed B cells from NP or tonsil, or after ILC2 coculture, by flow cytometry.

46 (ampicillin and chloramphenicol) so that the coculture can survive in antibiotic concentrations that

47 ulation of VEGFR2 by ADAM17 was confirmed in cocultured cardiomyocyte-fibroblast as ischemia-induced

48 glioma cells and normal rat astrocytes: CNS coculture caused quiescence and protection from methotre

49 ed stromal fibroblasts (CAFs) derived from a coculture cell model and clinical patient samples, we de

50 and without asthma, ex vivo ASM, mast cells, cocultured cells and in a mouse model system.

51 Furthermore, FGNs can also interact with cocultured cells by physical or chemical stimulation, wh

52 ngineered hydrogels modulate the function of cocultured cells in the absence of inductive cues, thus

53 Cocultured cells that were treated with pro-CD demonstra

54 ith the most dramatic reduction occurring in cocultured cells.

55 nic and proangiogenic potential of entrapped cocultured cells.

56 Using coculture conditions mimicking the proliferative lymph n

57 When cocultures contained both monocyte subsets, or when cond

58 Stromal coculture did not prevent leukemia cell cycle activity,

59 inhibition of Akt in DRG neuron-Schwann cell cocultures dramatically decreased MBP and P0 levels and

60 on of TH2 cytokines was reduced in DC-T-cell cocultures during immunotherapy.

61 In this coculture, E. coli and R. palustris resemble an anaerobi

62 and upregulation of regenerative factors in cocultured EC and PO, indicating a protective influence

63 explained by regulation of E-selectin on the cocultured EC.

64 Coculturing endothelial cells with astrocytes yielded th

65 Blocking IL-32alpha during DC:NK cell coculture enhanced NK cell effector molecule expression

66 ediated upregulation of ADIPOQ expression in cocultured EpAT.

67 14) or healthy control subjects (n = 6) were cocultured ex vivo in the presence of an S pyogenes extr

68 rons only from the same region in mismatched cocultures, exhibiting region-matched astrocyte to neuro

69 By employing a simple coculture experimental model and using single-molecule i

70 Coculture experiments revealed that prostate cancer cell

71 Coculture experiments revealed that the decrease in iNKT

72 We performed coculture experiments using healthy monocytes with exoso

73 However, in coculture experiments we observed that latent-infected c

74 salivaricin A and salivaricin B; however, in coculture experiments, GBS growth was impeded by K12 ind

75 chanisms of expression were assessed in cell coculture experiments, murine, and avian xenotransplants

76 In coculture experiments, T cells incubated with SEL24-B489

77 removal of 5'ppp, in vitro transcripts, and coculture experiments, we established that 5'pppEBER1 tr

78 (Treg) cells and mast cells were analyzed in coculture experiments.

79 mice stimulated collagen gene expression by cocultured fibroblasts.

80 In cocultures, fibrocytes from the mdx(5cv) diaphragm stimu

81 Neutrophil/A fumigatus coculture, flow cytometry, and video microscopy were use

82 ls were significantly higher in LX2 cells in coculture following HIV/HCV coexposure compared with eit

83 ne responses, donor C57BL/6 splenocytes were cocultured for 5 d with irradiated BALB/c splenocytes an

84 ects were most striking in myeloid DC-T-cell cocultures from subjects receiving OIT.

85 and the chemokine CXCL1 were upregulated in cocultured HT-29 cells at 4 h compared to levels in cont

86 Here, we examined the effects of coculturing human-derived adipocytes with established an

87 itioned supernatant from classical monocytes cocultures (IL-6(hi)) was added to nonclassical/intermed

88 Here, we engineered a micropatterned coculture (iMPCC) platform in a multiwell format that, i

89 THP-1 human monocytes and HMOBs were cocultured in a transwell system to investigate RANKL-dr

90 NSCs and endothelial cells (ECs), these were cocultured in an in vitro model in which NSC-induced end

91 When platelets were cocultured in the same compartment with lymphocytes, we

92 sponsiveness was observed when NK cells were cocultured in vitro with Influenza A/California/7/2009 v

93 We established cocultures in a microcosm model system to determine the

94 NCA on NF-kappaB activation in neutrophil/EC cocultures in vitro ANCA did not activate NF-kappaB in p

95 DIET have primarily been studied in defined cocultures in which Geobacter species are one of the DIE

96 However, this coculture increased CD39 expression.

97 In addition, IFNgamma blockade in T cell/VEC coculture increased VEC proliferation and VEGF-A protein

98 hibitors decreased cytokine production after coculture, indicating that Src is not only activated by

99 cells and fibroblasts compared with control cocultures induce more pronounced glycolytic differences

100 al keratinocytes the supernatants from these cocultures induced an increase in IL-17-associated genes

101 as inflammation studies, drug screening, and coculture interactions.

102 oreover, we find that daily dilutions of the coculture lead to large oscillations in the relative abu

103 ally, TIGIT signaling in NK cells after MDSC coculture led to a decrease in the phosphorylation of ZA

104 We created an in vitro coculture model and investigated the contributions of HI

105 brotic genes was significantly higher in the coculture model compared to either cell type in monocult

106 Importantly, our practical 3D coculture model could provide a valuable tool to underst

107 tablished an in vitro three-dimensional (3D) coculture model for the ocular surface.

108 was induced to mimic dry eye disease in the coculture model system.

109 c loss in HD, we developed a corticostriatal coculture model that features age-dependent dendritic sp

110 Using a coculture model to study the formation of bacterial biof

111 In a human coculture model, CLDN4-deficient astrocytes were unable

112 Utilizing a HPH-HepG2 coculture model, we demonstrate that inclusion of HPH co

113 Using a three-dimensional coculture model, we identified significant subtype-speci

114 iated dysfunction with disease; however such coculture models have randomly oriented myotubes with im

115 at intercellular mRNA transfer occurs in all coculture models tested (e.g., between primary cells, im

116 Using tissue and coculture models, we found that myofibroblasts and the f

117 human gut-on-a-chip microdevice was used to coculture multiple commensal microbes in contact with li

118 ferent regulators of neuronal morphology, we cocultured neuron explants with peripheral target tissue

119 aptic differentiation in contacting axons of cocultured neurons, similar to APP and other SAMs.

120 enhances neuron survival and axon growth of cocultured neurons.

121 Interestingly, coculture of AML cells with stromal cells increased auto

122 Coculture of cancer cells with dorsal root ganglion extr

123 fect was duplicated in tissue culture, where coculture of cancer cells with tumor-conditioned myeloid

124 Here we show that coculture of control astrocytes with neurons enhances ne

125 Coculture of DPSCs or I-DPSCs with differentiated THP-1

126 PE toxicity that was induced by NaIO3Ex vivo coculture of gammadelta T cells with RPE explants activa

127 h ROS, NFkappaB, and TGFbeta1 up-regulation; coculture of hepatocyte and hepatic stellate cell lines

128 We observed that coculture of human MSC and chondrocytes under standard c

129 nditional reprogramming (CR), which involves coculture of irradiated mouse fibroblast feeder cells wi

130 We have previously demonstrated that coculture of islets with mesenchymal stromal cells (MSCs

131 Coculture of live NTHi increased macrophage surface expr

132 Finally, coculture of malignant B cells with cells stably express

133 Coculture of mast cells with ASM activated by IL-33 incr

134 Coculture of monocytes with exosome-packaged HCV, cell-f

135 Coculture of MSCs with ECFCs transduced to knockdown BMP

136 We found that coculture of murine bone marrow cells with bladder tumor

137 ermits concurrent isolation, separation, and coculture of myocyte and nonmyocyte cardiac populations.

138 However, coculture of normal PBMCs with Env-expressing cells resu

139 Coculture of PBMCs from healthy subjects with HCV-infect

140 Coculture of PHKCs with immunocytes resulted in the upre

141 The coculture of platelets with lymphocytes in the presence

142 Coculture of the 2 cell populations results in competiti

143 Coculture of the AML cell lines MOLM-4, THP-1 or primary

144 Coculture of the osteotropic PC-3M-Pro4Luc2 PCa cells wi

145 The coculture of the two cell components leads to elevated s

146 Ex vivo isolation and coculture of these respective APCs from S. pneumoniae- o

147 Coculture of TIGAR overexpressing carcinoma cells and fi

148 Additionally, coculture of tonsillar cells with infected hepatoma cell

149 LL) samples mostly from resistant disease in cocultures of bone marrow stromal cells.

150 man cervical samples, with levels highest in cocultures of cervical fibroblasts and cancer-derived ep

151 same CXCR2 ligands was likewise increased in cocultures of early-passage cells from human cervical sa

152 en primary cells, immortalized cells, and in cocultures of immortalized human and murine cells).

153 (+) T cells was also replicated in vitro, in cocultures of macaque macrophages and CD4(+) T cells.

154 Cocultures of Pelobacter SFB93, a C2H2-fermenting bacter

155 notypic whole-cell screening in erythrocytic cocultures of Plasmodium falciparum identified a series

156 The cocultures of platelets with synovial fluid cells from r

157 To this end, we established transwell cocultures of rat hippocampal neurons and MSCs.

158 In cocultures of SF and Th cells, tryptophan was completely

159 Coculturing of EBV-positive B cells with OKF6 cells indu

160 istant phenotype in human MM cells that were cocultured on BM stroma.

161 mental approaches with a synthetic anaerobic coculture pairing fermentative Escherichia coli and phot

162 When we cocultured platelets with lymphocytes at different ratio

163 In coculture, PMFs developed intercellular junctions with E

164 We report here that corticostriatal cocultures prepared from YAC128 HD mice feature age-depe

165 We therefore cocultured primary circulating MCL cells from 21 patient

166 TAMs were generated by coculturing primary human macrophages (MPhi) with human

167 After coculture, recipient AML cells showed a 1.5-fold increas

168 xpression of CoL1A1 and TIMP1; and our novel coculture reporter cell model represents an efficient an

169 Coculturing RhoA knockout fibroblasts and PC3 cells indu

170 and the PDCD1LG2-IGHV7-81 fusion showed, in coculture, significantly reduced T-cell activation.

171 CD11c(+) cell-conditioned media or coculture stimulated fibroblast proliferation, activatio

172 ed contrast was further confirmed in in situ coculture studies using neutralizing CCR2 antibodies.

173 s was examined in keratinocytes treated with coculture supernatants.

174 In the absence of mesenchymal coculture support, this NKX2-1+ population was able to g

175 iments, flow cytometry, confocal microscopy, cocultures, suppression assays, real-time quantitative P

176 In motoneuron-astrocyte contact cocultures, synapse formation and synaptic transmission

177 oietic stem and progenitor cell/EC (HSPC/EC) coculture system as well as in vivo EC infusions followi

178 ti-plexed measurement of 48 cytokines from a coculture system of primary human CD4+ T cells and monoc

179 Our results showed that the coculture system provides a more physiologically relevan

180 Here we demonstrate that in a niche-like coculture system, cells from both primary and cultured a

181 n different experimental settings, such as a coculture system, sorted CD154(+) T cells, IFX peptide-s

182 Here, we show, using an ex vivo coculture system, that intermediate and poor risk AML pa

183 Using a coculture system, we determined that senescent cholangio

184 ming, based on an accelerated dendritic cell coculture system, which uses unfractionated human PBMCs

185 osures in a dorsal root ganglion organotypic coculture system.

186 Drug-stimulated PBMCs or coculture systems were used to detect memory T cells in

187 We also compared several coculture systems, and found that direct cell contact be

188 -cell suppression was assessed in allogeneic coculture systems.

189 Mechanistically, CD103(+) DCs caused cocultured T cells to differentiate into Tregs and produ

190 es of amino acid biosynthesis were higher in coculture than in axenic culture, and this was reflected

191 further implicate cell-cell communication by coculturing the fibroblasts with cancer cells, which we

192 activity in these neurons can be restored by coculturing them with normal rat hippocampal neurons, de

193 In cocultures they differentiated B cells into CD138(+) pla

194 Added to TFH cell and B-cell cocultures, they inhibited B-cell differentiation, imped

195 added to nonclassical/intermediate monocyte cocultures (TNF(hi)), the activating effects of TNF were

196 duced secretion of IL-1beta and TNF-alpha in cocultures, TNF-alpha blockade did not affect gp120-medi

197 lants to test monocyte adhesion and in vitro cocultures to evaluate CD8+ T cell function.

198 ployed physically separated neuron-astrocyte cocultures to investigate potential non-cell autonomous

199 Using 3D cocultures to mimic the cellular interactions of an emer

200 When ammonium cross-feeding was limited, coculture trends changed yet coexistence persisted under

201 mesenchymal stem cell features and protected cocultured tubule cells from cisplatin-induced apoptosis

202 In coculture, VWM astrocytes secreted factors that inhibite

203 production and release, whereas toxicity in coculture was lactate-independent, demonstrating that MN

204 WT) mice, as well as their mixed and matched cocultures, we characterized the contributions of motone

205 cells (BMDCs), and moDC/naive CD4(+) T-cell cocultures were analyzed by using ELISA and flow cytomet

206 , PNS-like axon bundles elaborated by apical cocultures were longer than their basal counterparts and

207 Human and mouse neutrophil-epithelial cocultures were used to evaluate the role of neutrophil-

208 IFN-gamma and 2-fold higher IL-17A in T cell cocultures), whereas cDCs induced 10-fold higher IL-2 pr

209 tion of esx4 genes in the recipient requires coculture with a donor strain and a functional ESX-1 app

210 Subsequent to hematopoietic induction, coculture with AGM AKT-ECs also substantially increased

211 s from 58 high-risk women was measured after coculture with antigen-presenting cells preincubated wit

212 upregulated in WAT-derived progenitors after coculture with breast cancer: granulocyte macrophage col

213 d activity of 5-LO in TAMs were reduced upon coculture with cancer cells.

214 reased, initiation of infection in trans via coculture with CD169(+) IFN-alpha-treated DCs restored i

215 ltrating Treg reside close to bile ducts and coculture with cholangiocytes or their supernatants indu

216 ursors and can be differentiated in vitro by coculture with developmentally supportive stromal cells.

217 vel of interleukin 1beta was not affected by coculture with DPSCs or I-DPSCs.

218 to 12-fold higher than those observed during coculture with EDL933 and Sakai, respectively.

219 cells expressing EPHB1 form aggregates upon coculture with ephrin B1 expressing cells.

220 ALL cells were analyzed in coculture with human glioma cells and normal rat astrocy

221 acrophages (MDMs) were studied in noncontact coculture with human MSCs when stimulated with LPS or br

222 crovascular ECs (pDMECs) to CGRP followed by coculture with LCs, responsive CD4(+) T cells and Ag res

223 We showed that coculture with lymphoid-like cells, but not stromal cell

224 mutualism, L. rostrata experiences stress in coculture with M. loti, and must adjust its metabolism a

225 wn axenically with B12 supplementation or in coculture with M. loti.

226 d NK cells that produced less IFN-gamma upon coculture with M2.

227 or chronic inflammation in vivo, as well as coculture with macrophages in vitro.

228 under hypoxic culture conditions and during coculture with mesenchymal stem cells that mimic the AML

229 e with S-type cells induces N-type invasion, coculture with N-type cells slows S-type invasion.

230 d by distinctive GABAA receptors and that in coculture with neurons, the oligodendrocytes bearing the

231 iation of hPSCs, our system does not require coculture with other cell types and relies on chemically

232 e compactly clustered tumor-cell colonies in coculture with PC3 cells, which might boost tumor stem-l

233 med by removal of pericardial AT and ex vivo coculture with pericardial AT and granulocyte progenitor

234 Thus, whereas coculture with S-type cells induces N-type invasion, coc

235 ated Mcl1 levels in CLL cells in vivo and in coculture with stromal cells.

236 -1alpha is transcriptionally regulated after coculture with stromal cells.

237 s in the pathology, we performed muscle cell coculture with the Abs.

238 Coculture with the AFT024 stroma cell line is capable of

239 However, during coculture with the nonpathogenic strain E. coli C600, PA

240 Conversely, fibroblasts in coculture with TIGAR overexpressing carcinoma cells indu

241 r were activated in ex vivo T-ALL cells, and coculture with tumor-associated, but not normal thymic D

242 d marked levels of IL-4/IL-13 (10-fold) when cocultured with A549 EC and IL-3, without exogenous alle

243 When cocultured with activated T cells in vitro, KSL and KLCD

244 A/CD45RO T cells generated in 9-day MLR were cocultured with anti-CD3 and autologous antigen presenti

245 es were also infected with NTHi in vitro and cocultured with autologous T cells.

246 ed with IgE-NIP-tetanus toxoid complexes and cocultured with autologous T cells.

247 They were cocultured with B cells to induce their terminal differe

248 tic activity was observed for CD8(+) T cells cocultured with BPTF-silenced cells.

249 ylation and cell growth in malignant B cells cocultured with CD40L-expressing stromal cells.

250 ecrease histamine secretion and subsequently cocultured with cholangiocytes or HSCs prior to measurin

251 ol, human lymphatic endothelial cells (LECs) cocultured with dermal fibroblasts spontaneously organiz

252 ined and remained stable in oligodendrocytes cocultured with dorsal root ganglion neurons.

253 When cocultured with Dsg2/green fluorescence protein-expressi

254 usually from umbilical veins or cell lines, cocultured with freshly isolated peripheral blood mononu

255 ntal setting where infected fibroblasts were cocultured with gammadelta cells in submicromolar concen

256 PMPs were cocultured with healthy donor peripheral blood-derived T

257 HCV-specific CD8 T cells were cocultured with Huh7.5 cells that were pulsed with titra

258 nal sinus fat cells (RSFC) were isolated and cocultured with human endothelial cells (EC) or podocyte

259 Conversely, when hOPCs were cocultured with human neurons, M3R antagonist treatment

260 amounts of IFN-alpha via TLR7 signaling when cocultured with infected cells.

261 ildren, and adults were challenged by RSV or cocultured with infected HEp-2 cells.

262 nt porcine IL-10 + IL-6 to mononuclear cells cocultured with LGG significantly enhanced IgA responses

263 TH17 cells were also cocultured with lung APC subsets to determine which of t

264 ulation and cytotoxic activity than NK cells cocultured with M1.

265 Also, CD56(dim) NK cells cocultured with M2 displayed lower degranulation and cyt

266 dingly, the blunted cytotoxicity of NK cells cocultured with MDSCs against tumor cells could be rever

267 When cocultured with microglia BV-2 cells exposed to G-CSF, d

268 thodically altered when neuron explants were cocultured with microisolates from disparate cochlear re

269 Fused myotubes, when cocultured with MNs, were able to form even larger NMJs.

270 chemotactic and proliferative responses when cocultured with MSCs but not with cardiac stromal cells.

271 NPIs cocultured with MSCs had greater cellular insulin conten

272 ed Runx2 and osteocalcin upregulation in OBs cocultured with MVNP-expressing OCLs.

273 an blood and intestinal organ cultures, then cocultured with naive and memory CD4(+) T cells obtained

274 al blood and palatine tonsils, expanded, and cocultured with naive B cells.

275 d IL-17 expression in malignant T cells when cocultured with nonmalignant T cells, indicating an indi

276 Notably, when HNSCC cells were cocultured with normal fibroblasts, they upregulated aut

277 and VGLUT1 were increased when neurons were cocultured with Notch ligands-expressing NIH3T3 cells.

278 ation were also observed when CLL cells were cocultured with nurselike cells.

279 ndothelial cells exposed to laminar flow and cocultured with pericytes confirmed that atrasentan redu

280 ion, GFP-nsp2 was detected in HEK-293T cells cocultured with recombinant PRRSV-infected MARC-145 cell

281 ation was increased (P < .05) in macrophages cocultured with saliva-stimulated lymphocytes from expos

282 (SEA)-nonreactive naive CD4 Tcon cells were cocultured with SEA-reactive allergen-nonspecific Treg o

283 When cocultured with stabilin-1(high) monocytes, IFN-gamma sy

284 s expressing CD11b were serially diluted and cocultured with susceptible cells to amplify virus.

285 mRNA expression were increased in VECs when cocultured with T cells from HR transplants compared wit

286 Medicago truncatula plants were cocultured with the AM fungus Rhizophagus irregularis un

287 However, macrophages cocultured with the clinical isolate of Acanthamoeba pro

288 When cocultured with THP-1, RANKL released by PGE2-stimulated

289 5, and monocyte chemotactic protein-1, when cocultured with trophoblasts compared with control macro

290 murine bone marrow-derived macrophages were cocultured with trophozoites of either the laboratory Ne

291 T cells were either cocultured with vascular endothelial cells (VECs) to ass

292 A mice compared with those in WT motoneurons cocultured with WT astrocytes.

293 nce of Treg are more mobile as compared with cocultures with conventional CD4(+) T cells and form DC-

294 In cocultures with dendritic cells and CD4(+) T cells, AvCy

295 e of gammadelta T cells in monocyte-depleted cocultures with DV-infected DC.

296 vivo Treatment of dendritic cell (DC)-T cell cocultures with IFN-alpha upregulated CD169 expression o

297 s switching to IgG2, and was reproducible in cocultures with neutrophils.

298 High levels of TNF were detected in cocultures with nonclassical/intermediate monocytes, the

299 ong STAT3 activation and IL-17 expression in cocultures with SEA-responsive nonmalignant T cells.

300 er se was significantly triggered only after coculturing with tumor cells.