ライフサイエンスコーパス: conserved residue

1 oskeletal GTPase Septin7, at an evolutionary conserved residue.

2 tion on lysine 403 (K403), an evolutionarily conserved residue.

3 ion in xanthophores, phosphorylates Plin6 on conserved residues.

4 ds the top face or to be at buried or highly conserved residues.

5 She2p binding pocket are composed of highly conserved residues.

6 ubules by using small groups of evolutionary conserved residues.

7 two families displaying MFSD2A mutations in conserved residues.

8 ally interacts with COQ7 through a series of conserved residues.

9 ysis reveals that prolines constitute 17% of conserved residues.

10 tinguish regulatory sites from nonfunctional conserved residues.

11 to a wide range of mutations, even in highly conserved residues.

12 e containing a Mn(2+) cluster coordinated by conserved residues.

13 sents a previously unknown key role of these conserved residues.

14 nt posttranslational modifications at highly conserved residues.

15 d specific interaction involving several Sox conserved residues.

16 fibronectin (FN1) variants affecting highly conserved residues.

17 trinuclear zinc center, coordinated by nine conserved residues.

18 bridges, or hydrophobic interactions between conserved residues.

19 Mutation of these conserved residues abolishes GluN2C binding and has no f

20 of hydrogen bond interactions between highly conserved residues across the RNase dimer interface that

21 the oxyanion hole, are among the most highly conserved residues across the YCII superfamily members.

22 es seems to be mediated by cation binding to conserved residues along the three-fold axis.

23 m, tyrosine 89, within the SH2bm is a highly conserved residue among IAV strains.

24 The missense mutation concerns a highly conserved residue among species, located in the region i

25 erefore, K8 acetylation at Lys-207, a highly conserved residue among type II keratins and other IFs,

26 The change is at a highly conserved residue and cosegregated with the phenotype in

27 Cysteine 115 is a highly conserved residue and forms a key part of a catalytic tr

28 ine functional roles for each of these eight conserved residues and allow us to propose a sequential,

29 : NM_001273.3), affect evolutionarily highly conserved residues and are predicted to be deleterious.

30 in binding produce different arrangements of conserved residues and customized interfaces on the hydr

31 ar head of H3 HA that is comprised of highly conserved residues and is distinct from the receptor bin

32 we have identified a surface patch of highly conserved residues and shown that those residues are ess

33 ent of the H-bonding network associated with conserved residues and structural water molecules.

34 up the recombinant protein when four highly conserved residues and the 70-amino acid small alpha+bet

35 The POLE mutations localized to highly conserved residues and were strongly predicted to affect

36 amino acid residues of the active quintet of conserved residues, and of surface-exposed residues for

37 Both mutations affected conserved residues, and R291Q is orthologous to R294, wh

38 We hypothesize that deleterious variants at conserved residues are enriched in severely affected pat

39 These conserved residues are likely to perform the same critic

40 Highly conserved residues are located in the extensive interfac

41 Evolutionarily conserved residues are optimally connected, defining a s

42 A highly conserved residue Arg(97) in the CDR3alpha loop played a

43 rent de novo mutations, affecting the highly conserved residue Arg138 of P5CS, cause an autosomal-dom

44 amino acid substitutions of the same highly conserved residue, Arg138 in P5CS.

45 Mutations of conserved residues around this pocket affect activity of

46 However, the non-conserved residues Asn-120, Arg-357, and Asn-373 form di

47 nsport cycle were examined, and the roles of conserved residues Asp(196), Glu(198), Lys(873), and Asn

48 ange is stabilized by the interaction of the conserved residues Asp(590) and Tyr(628) and results in

49 Site-directed mutagenesis of these highly conserved residues (Asp-192, Arg-258, Phe-272, Glu-295,

50 rvation of another LBHB (2.47 A) between two conserved residues, Asp233 and Asp246, suggests that LBH

51 Mutation of the conserved residues Asp370 and Glu372 in the beta3-alpha3

52 HA subtypes, including substitution of other conserved residues associated with receptor binding.

53 (-) and mutations of the proton glutamate, a conserved residue at the internal side of the protein.

54 structures suggest the involvement of highly conserved residues at the active site.

55 nine substitutions introduced to four highly conserved residues at the C terminus and one at the N te

56 that cdG binds to FliI in a pocket of highly conserved residues at the interface between two FliI sub

57 Of interest, the evolutionarily conserved residues at the luminal interface of the third

58 We hypothesized that the myristate and the conserved residues at the N terminus of L1 are critical

59 and found that they contained nearly all the conserved residues characteristic of SPaseII family memb

60 Conserved residues cluster around a loop we term the "K

61 expected under the assumption that the most conserved residue confers the highest fitness, there was

62 to identify signatures involving the role of conserved residues, conserved contacts, and downstream s

63 Substitution of the strictly conserved residue Cys-121 of FdhD impairs both sulfur tr

64 ovel molecular switch involving the strictly conserved residue D74(2.50).

65 This tight coordination by a network of conserved residues defines a sequential, around-the-ring

66 ore sequences, we were able to highlight key conserved residues driving essential elements of TCR rec

67 revealed dramatic changes to the exposure of conserved residues during virus maturation.

68 The five missense mutations affect highly conserved residues either in the sixth repeat of the RCC

69 ialized active site consisting of two highly conserved residues equivalent to SOD5 Glu-110 and Asp-11

70 We found that several evolutionally conserved residues, especially G228 and G229, appeared t

71 of these mutations were localized near or at conserved residues essential for diiron ion coordination

72 ltimate interaction we detected involves the conserved residue F402, which has hydrophobic contacts w

73 st, most missense mutations affecting highly conserved residues failed to eliminate ACC2 function.

74 stitution (p.Arg970Pro) at amino-acid 970, a conserved residue for the catalytic activity of AGTPBP1.

75 del in which backbone strain co-evolved with conserved residues for allosteric control of catalytic a

76 heet within an alpha+beta-fold to coordinate conserved residues for catalysis.

77 ng of all members of the genus also revealed conserved residues forming the structural basis of the E

78 ar beta-helical (solenoid) conformation with conserved residues forming the tightly packed core and p

79 Conserved residues from a diverse set of BAM2 orthologs

80 SB complex formation requires evolutionarily conserved residues from both proteins, including a pair

81 Conserved residues from the signal peptide and residues

82 croscopic analyses to demonstrate that these conserved residues function after assembling HIV-1 Gag h

83 n this study, we demonstrate that the highly conserved residue G33, located within domain 1 of the co

84 These studies also indicate that the conserved residue Glu-51 acts as the general base in the

85 rg62 forms a salt bridge with another highly conserved residue, Glu38.

86 Alanine substitution of the conserved residue H98 in prM disrupts the switch by inhi

87 individual BTHS mutations at evolutionarily conserved residues has identified seven distinct loss-of

88 sfer coupled with a proton transfer from the conserved residue, His548.

89 ular N-terminal core, a flexible linker, 8-9 conserved residues implicated in interactions with modul

90 on other NAOX-nucleic acid complexes reveals conserved residues important for recognition and demethy

91 The de novo variants affect a single conserved residue in a zinc finger motif crucial for DNA

92 by the orientation of arginine 66, a highly conserved residue in Anthozoan PCFPs.

93 se mutation c.591C>A p.Glu197Asp in a highly conserved residue in exon 4 of ACTA1.

94 Lysine 19 is a conserved residue in human glycine N-acyltransferase-lik

95 uenced by Polo-mediated phosphorylation of a conserved residue in Miro, which positively regulates Mi

96 Surprisingly, mutation of the conserved residue in Set2 (R195C) similarly resulted in

97 mical mechanism: direct phosphorylation of a conserved residue in the activation loop (Cdr2-T166 and

98 l analyses reveal that a single, differently conserved residue in the cap domain of either AUM or chr

99 .Thr270Ala missense variant affects a highly conserved residue in the DBL homology domain, which is r

100 C479 is a highly conserved residue in the extracellular domain of ENaC an

101 utagenic screen we identified K165, a highly conserved residue in the extracellular vestibule of the

102 T835M alters a conserved residue in the hinge region of UNC5C, and in v

103 ors, one of which (Arg303Cys) is at a highly conserved residue in the N/D loop.

104 Finally, we show that an evolutionarily conserved residue in the PLL domain is critical for olig

105 This A178D missense mutation, affecting a conserved residue in the second immunoglobulin-like doma

106 both in vivo and in vitro on an evolutionary conserved residue in the switch II domain.

107 phosphorylates a subset of Rab GTPases on a conserved residue in their switch-II domains (PMID: 2682

108 y an arginine-to-cysteine change at a highly conserved residue in tyrosinase-related protein 1 (TYRP1

109 y more new damaging and missense variants at conserved residues in cases than in controls (P = 1.6 x

110 Interestingly, there are six differentially conserved residues in DPs affording either the (+)- or (

111 Disease-associated point mutations of conserved residues in either the Rab3GAP1 (T18P and E24V

112 By targeting conserved residues in Escherichia coli RNAP, we are able

113 Here, we have mutated conserved residues in Fml1's Walker A (K99R) and Walker

114 BL patients, are either truncating or affect conserved residues in functional domains, thus supportin

115 en in the presence of extensive mutations to conserved residues in HA's receptor-binding pocket.

116 functional differences between positionally conserved residues in how they influence recombinase-tar

117 We show that ERK phosphorylates Dicer on two conserved residues in its RNase IIIb and double-stranded

118 ys, we performed an in vivo analysis of well-conserved residues in Msh3 that are hypothesized to be r

119 Alteration of highly conserved residues in PhoU by site-directed mutagenesis

120 erform structure-function analysis of highly conserved residues in Porcn and Wnt3a.

121 Many cohesinopathy mutations target conserved residues in Scc2 and diminish Ct Scc2 binding

122 y)-both affecting functionally important and conserved residues in SEC61.

123 unctional significance of two evolutionarily conserved residues in SETD2 that are recurrently mutated

124 s O-linked mannose (O-Man) glycans at highly conserved residues in specific extracellular cadherin do

125 s) holds that there are several evolutionary conserved residues in TCR variable regions that contact

126 showing its architecture and the position of conserved residues in the active site.

127 By mutating conserved residues in the beta-barrel domain of this pro

128 TPP enzymes with an essential role for some conserved residues in the catalytic domain.

129 erpesviruses revealed the presence of highly conserved residues in the central portion of the UL37 pr

130 We report that mutation of three strongly conserved residues in the ectodomain has no effect on ta

131 We identified two evolutionarily conserved residues in the FERM (4.1 protein and ERM) dom

132 We focused on two highly conserved residues in the HA stem region: HA2 position 5

133 Previous studies have shown that highly conserved residues in the inner domain of gp120 are requ

134 NCT variants that harbor mutations at highly conserved residues in the lid region inNCT-deficient cel

135 By revealing precisely when conserved residues in the major homology region are requ

136 Highly conserved residues in the major homology region are requ

137 Herein, we targeted conserved residues in the MeaB switch I motif to interro

138 Collectively, our results identify conserved residues in the membrane-proximal region of CN

139 mmunoelectron microscopy to demonstrate that conserved residues in the MHR are required after assembl

140 Using yeast coa6Delta cells, we show that conserved residues in the motif, including the residue m

141 These residues are the only conserved residues in the OSBS family, so they are not s

142 These amino acid substitutions affect well-conserved residues in the prodomain and in the peptidase

143 esis-based approach, we demonstrate that the conserved residues in the putative cyclin-binding motif

144 rus reveals that the antibody targets highly conserved residues in the receptor binding site and cont

145 that the intermolecular interactions between conserved residues in the RNase domain are required for

146 Substitution of conserved residues in the SAM binding pocket reveals a f

147 the mechanism of Erv14 function, we identify conserved residues in the second transmembrane domain of

148 with CD147(CG) was inhibited by mutations to conserved residues in their lectin domains.

149 Accordingly, single mutation of specific conserved residues in these motifs, whilst irrelevant in

150 The strictly and strongly conserved residues in this domain cluster in a single ar

151 The presence of several conserved residues in this region together with these st

152 formation by demonstrating that defined non-conserved residues in TM3 and ECL2 of classic claudins c

153 Mutation of highly conserved residues in transcription factors may affect p

154 imer interface and involves several strictly conserved residues, including Arg-60.

155 Mutation of any of the conserved residues increases nuclear accumulation of Htt

156 can be suppressed by phosphorylation of core conserved residues inside the SNARE domain.

157 y defined Munc13-1 C2A domain dimer revealed conserved residues involved in CAPS dimerization.

158 Substitution of conserved residues involved in either metal or nucleotid

159 The third conserved residue is a Cys within the beta-chain (betaCy

160 Conserved residues just past the recoding site are impor

161 electrostatic interactions with clusters of conserved residues, K326, K328, and R147, on actin.

162 2.Pi state, in which D714 interacts with the conserved residue K693, which possibly stimulates Zn(2+)

163 Most changes in the predicted conserved residues K76, R79, G81, and S83 produce no det

164 n amino acid structurally different from the conserved residue leads to the degradation of RT and, in

165 tion of these interaction sites and of other conserved residues leads to decreased DnaB helicase load

166 Substituting the ubiquitously conserved residue leucine 29 to alanine in the pore-form

167 A missense substitution of a highly conserved residue likely to affect the interaction of eI

168 Mutations at non-conserved residues lining the putative binding pocket of

169 le PASTA domain or by mutation of individual conserved residues lining the putative ligand-binding su

170 The role of eight invariant and highly conserved residues localized to the active site was inve

171 able LQTS-susceptibility gene and involves a conserved residue localizing to the proline, gltamic aci

172 Moreover, W69, a highly conserved residue located at the interface between layer

173 This mutation affects a highly conserved residue located in the second zinc finger doma

174 t PKD1 phosphorylation at Ser(203), a highly conserved residue located within the PKD1 N-terminal dom

175 type specificity because they bind to highly conserved residues located in the channel's central cavi

176 Here we identified highly conserved residues located on this amino acid loop criti

177 Mutations affect conserved residues located within Ca(2+)-binding loops I

178 The role and function of the conserved residue Lys 73 in the catalytic mechanism of c

179 Mutating these highly conserved residues markedly reduces c-di-GMP binding and

180 lower magnitude compared with CNIH-3, these conserved residues mediate a direct interaction between

181 Mutation of the conserved residues mediating the D5 interaction (Thr446

182 A pivotal role for the conserved residue N133 is suggested and further supporte

183 b2b) gene as a missense mutation at a highly conserved residue (N247S).

184 genomics and substituted with evolutionarily conserved residues (N251D, A263S, I299L, F387L, I476V, a

185 d to form a new H-bond with another strictly conserved residue, N46(1.50).

186 Here we report that mutation of conserved residues near and distant from the G(447) down

187 Point mutations at two moderately conserved residues near the Vik1 C terminus impaired mic

188 These results indicate that a highly conserved residue of an ABC transporter plays an importa

189 a single novel missense variant in a highly conserved residue of FLNC (filamin C; p.V2297M).

190 13 that are all phosphorylated at the highly conserved residue of serine 111 (Ser(111)) in response t

191 variant, c.112G>C (p.Gly38Arg), affecting a conserved residue of SLC39A8.

192 ygous missense mutation at an evolutionarily conserved residue of the C10orf2 TWINKLE gene.

193 el with an RNase H2 AGS mutation in a highly conserved residue of the catalytic subunit, Rnaseh2a(G37

194 Previous studies identified a highly conserved residue of the inner domain, W69, as being inv

195 mTOR are phosphorylated on an evolutionarily conserved residue of their ATP-binding domain.

196 introduced mutations in noncanonical and in conserved residues of either of the two nucleotide bindi

197 nt in soybeans with deleterious mutations at conserved residues of GmSACPD-C.

198 missense mutations were identified at highly conserved residues of lysyl-tRNA synthetase (KARS): the

199 Mutation of 23 highly conserved residues of NisB identified a number of amino

200 Similarly, the most conserved residues of orthologous and paralogous protein

201 milar studies of mutagenesis of structurally conserved residues of other tryptophylquinone enzymes.

202 Both DNA variants affect highly conserved residues of S1PR2 and are predicted to be dama

203 he APC/C requires its coactivator as well as conserved residues of the E2 and ubiquitin.

204 fferent de novo missense mutations involving conserved residues of the four GSK3 phosphorylation moti

205 to the identification of coding mutations in conserved residues of the melanocortin 4 receptor (MC4R)

206 tive pressure we observe a shift of the most conserved residues of the N-terminal helices.

207 interface of the Rev1-BRCT domain comprises conserved residues of the outer surface of the alpha1-he

208 dorferi clones containing point mutations in conserved residues of the putative TPR motif of BB0238 d

209 The mutation alters several conserved residues of the third intracellular loop, hamp

210 fied in these individuals localize to highly conserved residues of this WD-40-repeat-containing prote

211 chymotrypsin homologs that lack one or more conserved residues of typical trypsins and chymotrypsins

212 ein kinase catalytic domain contains several conserved residues of unknown functions.

213 To accomplish this, we have identified a non-conserved residue on the small subunit of all caspases t

214 eraction interface maps to a patch of highly conserved residues on B', which when mutated render B' i

215 imer with internal symmetry, and we identify conserved residues on the surface and within the dimeriz

216 Binding and actin-assembly assays show that conserved residues on the surface of this domain mediate

217 Mutagenesis of conserved residues on two remote surfaces of the NEL dom

218 Additionally, mapping of conserved residues onto the HAstV CP core and spike doma

219 Mutation of Arg-96, a poorly conserved residue opposite the loop, was unexpectedly fo

220 In addition, we identified a conserved residue outside the binding pocket (Trp-409) p

221 ified a missense mutation affecting a highly conserved residue (p.F90L) in the CALM1 gene encoding ca

222 trans in the S state but that two absolutely conserved residues (P14 and P101) become cis in the D st

223 tural analysis reveals key interactions with conserved residues P95 and W229 of importance for design

224 homologous protein models identified highly conserved residues, particularly at the azole binding si

225 A highly conserved residue, Phe396, appears to serve as the confo

226 long to the Ig-fold superfamily, the sets of conserved residue positions and identities differ betwee

227 posttranslational hydroxylation of a highly conserved residue (Pro-62) in the small ribosomal protei

228 eta-hairpin with a turn formed by the highly conserved residues Pro76 and Gly77.

229 Conserved residue proline 25 is involved in sequential u

230 Moreover, substitution of His-223, a conserved residue proposed to activate water in other am

231 her with biochemical data, indicate that the conserved residue Q146 in the flexible loop of HIV-1 int

232 Here, we identified two conserved residues (R151, I155) in the syntaxin-1 linker

233 This conserved residue represents a site of TARP action, inde

234 Mutation of a conserved residue required for sialic acid binding by ot

235 Biochemical and structural studies identify conserved residues required for this interaction and tra

236 odel that exploits knowledge of structurally conserved residue-residue interactions in the coiled-coi

237 ite-directed mutagenesis of gammaherpesvirus conserved residues revealed functional domains of these

238 hosphorylation by CSNK1A1 on a set of highly conserved residues (S824-S834), followed by rapid dephos

239 l fractions and is not phosphorylated at two conserved residues (Ser(338) or Thr(197)).

240 PHLPP1 dephosphorylated SGT1 at four conserved residues (Ser-17, Ser-249, Ser-289, and Thr-23

241 Soybeans carrying Gmsacpd-c mutations at conserved residues showed the highest stearic acid conte

242 ies of the autotransporter Pet show that the conserved residues significantly quicken completion of t

243 nstrate that DoS form sparse networks of non-conserved residues spanning distant regions.

244 cade of interactions predominantly involving conserved residues such as V139, D148, R167 and K155.

245 Mutagenesis data and the pattern of conserved residues suggest that there is probably not a

246 uence alignments and the locations of highly conserved residues suggest the presence of a dynamic lat

247 Conserved residues surrounding the pore have alternate s

248 ss-of-function ESR1 mutation in a completely conserved residue that interferes with estrogen signalin

249 missense mutation (p.Arg2024Gln) in a highly conserved residue that is essential for carbamoyl-phosph

250 enzyme is compared with mutants at Tyr68, a conserved residue that is located behind the reactive su

251 We also identify a conserved residue that is not required for ATPase activi

252 We conducted mutagenesis studies on several conserved residues that are considered critical for chlo

253 tures of NavAb, with helix bending involving conserved residues that are critical for slow inactivati

254 he binding affinity of ACK relies on several conserved residues that are critical for stabilizing the

255 We performed site-directed mutagenesis of conserved residues that are located in exposed regions o

256 shots have revealed several different highly conserved residues that are prominently inserted into th

257 The mutations affect a triplet of conserved residues that are situated neither in canonica

258 We report the importance of conserved residues that contribute to the overall charge

259 ere used to define the functions of the five conserved residues that define the FakB protein family (

260 ded through the channel by interplay between conserved residues that determine proton rejection and s

261 ngs, is facilitated by an insertion loop and conserved residues that hold the 3' primer terminus, and

262 In contrast, a set of conserved residues that reside at the junction between t

263 e superfamily, members of which use a set of conserved residues to catalyze a wide variety of reactio

264 This study investigates the role of a highly conserved residue, tryptophan residue 420, of the viral

265 Mutation of conserved residues typically involved in glucosyltransfe

266 phosphate additionally required active site conserved residues Tyr(40), Asp(181), and Arg(100)and a

267 transmembrane domains are composed of highly conserved residues, underlining their functional relevan

268 rm10 and variants with alterations in highly conserved residues, using crystal structures solved in t

269 Well conserved residues were highlighted, and the potential s

270 f apelin-36 variants in which evolutionarily conserved residues were mutated, and evaluated their abi

271 erm lineage provided evidence that W324 is a conserved residue, whereas the position equivalent to H5

272 onsible encodes the substitution of a highly conserved residue, which lies outside the benzamide-bind

273 Five highly conserved residues, which form a two-carboxylate clamp t

274 nel of the 40S subunit and contacts mRNA via conserved residues whose functional importance was unkno

275 orters and from the analysis of mutations of conserved residues will improve the understanding of the

276 findings identify a discrete pair of highly conserved residues with an essential role for controllin

277 Mutagenesis of conserved residues with potential catalytic function ide

278 p.Val404Met is novel and occurs at a highly conserved residue within the C-terminal end of the trans

279 ense substitution (c.5560G>A; p.D1854N) at a conserved residue within the catalytic domain.

280 The mutation is at a highly conserved residue within the DNA binding domain.

281 mutation, c.1532G>A (p.Arg511His), alters a conserved residue within the TBC1 domain.

282 Two of these variants also involve conserved residues within Cav1.2's PEST domain.

283 omatic NEK9 mutations, each affecting highly conserved residues within its kinase or RCC1 domains, in

284 Mutation of conserved residues within Rrp6 and Mtr4 at the structura

285 ecedented substituents able to interact with conserved residues within the ATP-binding site.

286 ids from the carboxyl tail, including highly conserved residues within the catalytic domain, plus a c

287 The roles of conserved residues within the cNOR family were investiga

288 in sequences preceding the first C of highly conserved residues within the CX5C or CX3H regions or wi

289 al of the C-tail alone or mutation of highly conserved residues within the domain still allows signif

290 We identify conserved residues within the LRRK2 ankyrin domain that

291 after the SP (HRASP), glycosylation and the conserved residues within the N-terminus in GLP-1R traff

292 hermore, site-directed mutagenesis of highly conserved residues within the PepSY domains resulted in

293 We show that mutation of conserved residues within the Rcf1 QRRQ motif alters the

294 the effects of alanine substitutions at many conserved residues within the SSP on viral replication i

295 d a systematic mutagenesis at the flavivirus conserved residues within the TMDs of NS2B.

296 es adjacent to the phosphorylation sites and conserved residues within the tri-helix bundle.

297 We conclude that, although the majority of conserved residues within the TZF domain of TTP are requ

298 lographic and mutational studies reveal that conserved residues within the UBN1-HRD and H3.3 G90 as k

299 hysiological characterization shows that the conserved residues within transmembrane segments 2 and 7

300 Site-specific mutational analyses of the conserved residues within WRDPLVDID indicated that Trp-6