ライフサイエンスコーパス: cysteine desulfurase

1 cture-function properties of a mitochondrial cysteine desulfurase.

2 and the adjacent gene, nifZ, encoding for a cysteine desulfurase.

3 IscS is one of the three Escherichia coli l-cysteine desulfurases.

4 s unknown, and many archaea lack homologs of cysteine desulfurases.

5 lfur clusters is derived from L-cysteine via cysteine desulfurases.

6 layed both SufE activity (stimulating CpNifS cysteine desulfurase activity 70-fold) and quinolinate s

7 S homologs, csdA and csdB, each of which has cysteine desulfurase activity and could potentially dona

8 t proteins were similar to wild type in both cysteine desulfurase activity and sulfur transfer to Isc

9 at, of the multiple proteins in E. coli with cysteine desulfurase activity as observed by native gel

10 We postulate that the inhibition of cysteine desulfurase activity by IscX serves to reduce u

11 In Escherichia coli, the major cysteine desulfurase activity for biogenesis of iron-sul

12 urified recombinant SufE2 could activate the cysteine desulfurase activity of CpNifS 40-fold.

13 derived from the amino acid cysteine by the cysteine desulfurase activity of Nfs1.

14 complex act synergistically to modulate the cysteine desulfurase activity of SufS.

15 port that the SufE protein can stimulate the cysteine desulfurase activity of the SufS enzyme up to 8

16 esidues 376-413 (IscSDelta376-413) displayed cysteine desulfurase activity similar to the full-length

17 formation of a ternary complex with reduced cysteine desulfurase activity, and we determined a low-r

18 se data support the conclusion that, via its cysteine desulfurase activity, IscS provides the sulfur

19 sphate (PLP)-dependent enzyme with intrinsic cysteine desulfurase activity, no evidence for PLP bindi

20 h as a donor of Fe(2+) and as a regulator of cysteine desulfurase activity.

21 PP)-conjugated acyl chain to support maximal cysteine desulfurase activity.

22 ion, whereas ferrous iron further stimulates cysteine desulfurase activity.

23 e, and had both cysteine sulfoxide lyase and cysteine desulfurase activity.

24 he proteins and assayed their effect on SufS cysteine desulfurase activity.

25 in archaea is not known as many archaea lack cysteine desulfurase and an RLD of the putative ThiI.

26 ssense mutations exhibited similar losses of cysteine desulfurase and Fe-S cluster assembly activitie

27 Our analysis revealed Lecsl to be a novel cysteine desulfurase and not a type of cysteine sulfoxid

28 anistically unified by the requirement for a cysteine desulfurase and the participation of an [Fe-S]

29 ck of the gene encoding for a canonical IscS cysteine desulfurase and the presence of a short sequenc

30 ) system consisting of two components: NifS (cysteine desulfurase) and NifU (scaffold protein).

31 IscS, a cysteine desulfurase, appeared to be an exception: altho

32 Although cysteine desulfurases are conserved from bacteria to hum

33 ied the (35)S-labeled persulfide on the NFS1 cysteine desulfurase as a genuine intermediate en route

34 persulfide intermediate in the reaction of a cysteine desulfurase (CD), CD0387 from Synechocystis sp.

35 Cysteine desulfurases (CDs) are pyridoxal-5'-phosphate (

36 ed the two constituent proteins of the human cysteine desulfurase complex (NFS1 and ISD11) separately

37 er on the scaffold protein (ISCU) involves a cysteine desulfurase complex (NFS1/ISD11) and frataxin (

38 ion into multiple biosynthetic pathways by a cysteine desulfurase complex that consists of a catalyti

39 In vitro, the chloroplastic cysteine desulfurase CpNifS can release elemental sulfur

40 Cysteine desulfurases, designated NifS, IscS, and SufS,

41 Here we show that cysteine desulfurase encoded by the gene iscS of E. coli

42 The cysteine desulfurase enzymes NifS and IscS provide sulfu

43 h SufE and IscU with the mutually homologous cysteine desulfurase enzymes present in their respective

44 Nfs1p is homologous to cysteine desulfurase enzymes that function in iron-sulfu

45 he catalytic efficiency (k(cat)/K(M)) of the cysteine desulfurase from 25 to 7900 M(1)s(1).

46 D-0387), a sequence group I (NifS/IscS-like) cysteine desulfurase from Synechocystis sp. PCC 6803, by

47 und that the iscS gene, a member of the nifS cysteine desulfurase gene family, is required for 4-thio

48 eria, including Bacillus subtilis, contain a cysteine desulfurase gene sufS located adjacent to the g

49 IscS, a cysteine desulfurase implicated in the repair of Fe-S cl

50 Cysteine desulfurases in their persulfurated forms serve

51 erase, receiving the sulfur donated from the cysteine desulfurase IscS and transferring it to the tar

52 The Escherichia coli L-cysteine desulfurase IscS mobilizes sulfur from L-cystei

53 following two enzymes for its synthesis: the cysteine desulfurase IscS, which forms a Cys persulfide

54 both the thiamin pathway enzyme ThiI and the cysteine desulfurase IscS.

55 y requires MnmA, Mg-ATP, l-cysteine, and the cysteine desulfurase IscS.

56 ons share strong sequence homology, e.g. the cysteine desulfurases IscS and SufS, and presumably play

57 IscX binds iron ions and interacts with the cysteine desulfurase (IscS) and the scaffold protein for

58 reduce sulfane sulfur (S(0)) produced by the cysteine desulfurase (IscS) to sulfide (S(2-)) as requir

59 The results suggest that cysteine desulfurase (IscS) together with L-cysteine can

60 scU in the presence of the iron-loaded IscA, cysteine desulfurase (IscS), and L-cysteine, demonstrati

61 orms a stable complex in vivo with the human cysteine desulfurase (ISCS), which generates the inorgan

62 ter, we identified and characterized the Mtb cysteine desulfurase (IscS; Rv3025c) and developed a nat

63 es place in the complex between IscU and the cysteine desulfurase, IscS, and our NMR studies demonstr

64 The human cysteine desulfurase, ISCS, has two isoforms, one of whi

65 The cysteine desulfurase, IscS, provides sulfur for Fe-S clu

66 tudies showed that TUM1 interacts with the l-cysteine desulfurase NFS1 and the rhodanese-like protein

67 unexplained processing of the mitochondrial cysteine desulfurase Nfs1 in yeast.

68 r example, the assembly process requires the cysteine desulfurase Nfs1, which serves as the sulfur do

69 Frataxin interacts with Isu, iron, and the cysteine desulfurase Nfs1, which supplies sulfide, thus

70 el, that is dependent on the presence of the cysteine desulfurase Nfs1.

71 ter assembly; this complex also includes the cysteine desulfurase (Nfs1 in yeast) and the accessory p

72 rganisms, tRNA thiolation is mediated by the cysteine desulfurase, Nfs1 (IscS).

73 r modeling suggests that two subunits of the cysteine desulfurase, Nfs1, may bind symmetrically on to

74 In Saccharomyces cerevisiae, the cysteine desulfurase Nfs1p and an accessory protein, Isd

75 evidence for PLP binding or for PLP-induced cysteine desulfurase or biotin synthase activity was obs

76 Cysteine desulfurases perform pyridoxal phosphate (PLP)-

77 l-Cysteine desulfurases provide sulfur to several metaboli

78 Increasing evidence suggests that IscS, a cysteine desulfurase, provides sulfur for assembly of tr

79 e active role for SufE in promoting the SufS cysteine desulfurase reaction for Fe-S cluster assembly.

80 NifS is a cysteine desulfurase, releasing sulfur or sulfide (depen

81 Nfs1, the cysteine desulfurase responsible for providing sulfur fo

82 hesized in a multistep process that utilizes cysteine desulfurases, scaffold proteins, chaperones, an

83 olves interaction of Isu with both Nfs1, the cysteine desulfurase serving as a sulfur donor, and the

84 The Escherichia coli cysteine desulfurase SufS (EC 2.8.1.7) and its accessory

85 on starvation in Escherichia coli involves a cysteine desulfurase SufS.

86 , the cytosolic form of ISCS is a functional cysteine desulfurase that can collaborate with cytosolic

87 IscS is a widely distributed cysteine desulfurase that catalyzes the pyridoxal phosph

88 odimeric IscS protein has been shown to be a cysteine desulfurase that catalyzes the reductive conver

89 that the cytosolic form of ISCS is an active cysteine desulfurase that covalently binds 35S acquired

90 IscS from Escherichia coli is a cysteine desulfurase that has been shown to be involved

91 proteins specifically associate with IscS, a cysteine desulfurase that is proposed to sequester inorg

92 port cloning of the human homolog of NifS, a cysteine desulfurase that is proposed to supply the inor

93 as IscS, a recently characterized NifS-like cysteine desulfurase that mobilizes sulfur for the synth

94 hat decreased expression of Nfs1p, the yeast cysteine desulfurase that plays a central role in Fe-S c

95 NifS is a homodimeric cysteine desulfurase that supplies the inorganic sulfide

96 Unlike prokaryotic cysteine desulfurases, the SDA structure adopts an unexp

97 r source, and the sulfur is transferred from cysteine desulfurase through a persulfidic intermediate

98 Lecsl and that the gene Csl encoded a novel cysteine desulfurase to influence organosulfur compounds

99 Recently, a cysteine desulfurase was reported in Arabidopsis (Arabid

100 of IscS (SA1450) in S. aureus is active as a cysteine desulfurase, which enables the in vitro reconst