ライフサイエンスコーパス: disulfide bridge

1 zed, and its structure was stabilized with a disulfide bridge.

2 SDP is a 42-residue peptide with one disulfide bridge.

3 conformation with an engineered interdomain disulfide bridge.

4 sults in homodimerization of Src linked by a disulfide bridge.

5 subunits are glycosylated and connected by a disulfide bridge.

6 as completely reversed upon reduction of the disulfide bridge.

7 utants reveals that the two cysteines form a disulfide bridge.

8 isulfide bonds and homodimerizes via another disulfide bridge.

9 during oxidation to form the Cys-2 to Cys-7 disulfide bridge.

10 ng conditions, confirming the existence of a disulfide bridge.

11 ed clamshell conformation by incorporating a disulfide bridge.

12 isely positioned cysteines that form a third disulfide bridge.

13 site Arg-122, and restoration of the missing disulfide bridge.

14 ed to be a homotetramer with an intersubunit disulfide bridge.

15 d Cu(I) ions over forming the intramolecular disulfide bridge.

16 ow pH, proteolysis, and an intact interchain disulfide bridge.

17 paclitaxel (PTX) to vitamin E (VE) through a disulfide bridge.

18 mposed of two subunits linked by a conserved disulfide bridge.

19 lin-like domains containing three intrachain disulfide bridges.

20 a 62-amino acid peptide cross-linked by five disulfide bridges.

21 ry structure and experimental constraints on disulfide bridges.

22 We discuss the biological relevance of such disulfide bridges.

23 5.3 kDa), with six cysteine residues forming disulfide bridges.

24 revealed an important structural role of the disulfide bridges.

25 sequence but also on the localization of the disulfide bridges.

26 loped, comprising 12-16 amino acids with two disulfide bridges.

27 s, approximately 50% of which were linked by disulfide bridges.

28 oxin that blocks nAChRs and does not possess disulfide bridges.

29 was no evidence for intermolecular FLS2-FLS2 disulfide bridges.

30 sically disordered protein that can form two disulfide bridges.

31 tory C-terminal tail and stabilized by three disulfide bridges.

32 tion-pi interactions, but not intramolecular disulfide bridges.

33 nterface of sfALR, close to the intersubunit disulfide bridges.

34 gh-molecular-weight complex held together by disulfide bridges.

35 ikely representing enzyme homodimers held by disulfide bridges.

36 red, including efficient mapping of multiple disulfide bridges.

37 ent collagen triple helix to form interchain disulfide bridges.

38 not be applicable when target proteins have disulfide bridges.

39 that is tethered to the C-terminal stalk by disulfide bridges.

40 three intramolecular and one intermolecular disulfide bridges.

41 ix core that is stabilized by four conserved disulfide bridges.

42 receptor (TIR)-domain fold stabilized by two disulfide bridges.

43 ly, fibrillation is robust to interchange of disulfide bridges.

44 nsulin, a two-chain protein containing three disulfide bridges.

45 particle formation by forming intermolecular disulfide bridges.

46 ee structurally indispensable intramolecular disulfide bridges.

47 the two alpha-helices are linked by the two disulfide bridges.

48 ns are larger (31-36 amino acids) with three disulfide bridges.

49 e-rich trypsin inhibitor MCoTI-II with three disulfide bridges.

50 ntiparallel beta-strands stabilized by three disulfide bridges.

51 or seven different peptides with two to four disulfide bridges.

52 ble, as needed to design the state-selective disulfide bridges.

53 inally amidated peptide cross-linked by four disulfide bridges.

54 ing agents due to the formation of nonnative disulfide bridges.

55 and alpha6 (107-125), held together by three disulfide bridges: 19-54, 50-108 and 97-117.

56 seven-transmembrane (TM) receptor conserved disulfide bridge (7TM bridge) linking transmembrane heli

57 Linker immobilization via an artificial disulfide bridge abolishes chaperone activity at elevate

58 of cysteine residues forming the C-terminal disulfide bridge abrogates pilus assembly, in turn elimi

59 ed a trapped dimer (dCXCL1) by introducing a disulfide bridge across the dimer interface.

60 LC translocation; premature reduction of the disulfide bridge after channel formation arrests translo

61 illustrated by the synthesis of symmetrical disulfide-bridged alpha PNA dimers containing 10 nucleob

62 conformationally restrained by three or more disulfide bridges, although recent findings by several g

63 yldithio)ethanamine hydrochloride formed the disulfide bridge and provided the terminal amine group,

64 give discrete products while maintaining the disulfide bridge and thus peptide conformation.

65 generally basic peptides with three or four disulfide bridges and a molecular mass of ~5 kDa.

66 e binding cavity by a pair of closely spaced disulfide bridges and a short helical segment within the

67 Five disulfide bridges and a Zn-binding site confer stability

68 te the importance of conserved extracellular disulfide bridges and aromatic residues in extracellular

69 nserved tertiary structure stabilized by two disulfide bridges and direct the migration of leukocytes

70 eted proenzyme formed non-native, interchain disulfide bridges and displayed only residual TPP I acti

71 have employed structure-based engineering of disulfide bridges and native mass spectrometry to show t

72 hydrogen bonds, supplemented by two proposed disulfide bridges and receptor-ligand contacts, derived

73 e describe the precise connection of all the disulfide bridges and show that the IgG2 C H1 and C-term

74 with the formation of interchain, nonnative disulfide bridges and the establishment of molten globul

75 fide linkages, particularly, for intertwined disulfide bridges and the unexpected disulfide scramblin

76 The two alpha-subunits are connected by a disulfide bridge, and both alpha- and beta-subunits are

77 Each CH3 monomer contains a conserved buried disulfide bridge, and we find that the successive reduct

78 as long as bovine chymotrypsinogen, has less disulfide bridges, and is a single polypeptide.

79 retro-translocates with oxidized intrachain disulfide bridges, and only upon proteasomal inhibition

80 salt bridges and side-chain hydrogen bonds, disulfide bridges, and propensities to form alpha-helice

81 te that all residues in this region can form disulfide bridges, and that the percentage of dimers inc

82 er Cys residues were found to be involved in disulfide bridges, and these are necessary for correct f

83 tural characterization of peptides including disulfide bridges annotation in the sequence.

84 loop 2 in conjunction with two extracellular disulfide bridges appeared to open and shape the entranc

85 rified this proximity by using an engineered disulfide bridge approach.

86 nce of the Cys-rich SMB domain, how its four disulfide bridges are arranged in the molecule remains h

87 Disulfide bridges are commonly found covalent bonds that

88 osslinking in reducing conditions shows that disulfide bridges are involved in KCC2 dimerization.

89 Subsequently, reductant is removed and all disulfide bridges are reoxidized to reform covalent inte

90 tudy protein fibrillization, since its three disulfide bridges are retained in the fibrillar state an

91 These disulfide bridges are unique for the known homophilic in

92 energetically preferred; however, all eight disulfide bridge arrangements are essentially possible.

93 ree-stranded antiparallel beta-sheet and the disulfide bridge array typical of vertebrate beta-defens

94 ins and is shown to be fully compatible with disulfide bridges, as evidenced by the selective modific

95 e normally rigid intramolecular Cys57-Cys146 disulfide bridge assumed two conformations.

96 prised of covalent DSP dimers connected by a disulfide bridge at Cys205.

97 her with alternating homodimer formation via disulfide bridges at the C-terminal Fn tail, should lead

98 is 132 amino acids in length, possesses five disulfide bridges at the C-terminus of the molecule, and

99 he thermal denaturation data point shows the disulfide bridges being responsible for the stability of

100 chains of NC2, a stable heterotrimer with a disulfide bridge between alpha1 and alpha3 chains is for

101 A disulfide bridge between C51a and C113, unique to the as

102 A double mutation that removes the disulfide bridge between C744 and C798 gives rise to gre

103 there is also evidence for an intramolecular disulfide bridge between consecutive cysteine residues.

104 ith mass spectrometry analysis showed that a disulfide bridge between Cys(499) and Cys(587) is centra

105 evidence suggests strongly that NO induces a disulfide bridge between Cys-110 and Cys-132 in intact c

106 The N and C termini are linked by a disulfide bridge between Cys1 and Cys158.

107 A noncanonical disulfide bridge between Cys2 and Cys99 stabilizes a lon

108 A disulfide bridge between Cys282 (the "x" of the SxN moti

109 The N-terminal loop, caused by a disulfide bridge between cysteines at positions 1 and 7,

110 loenzyme, required a covalent intermolecular disulfide bridge between GCLC and GCLM.

111 referred to as class A carbapenemases, is a disulfide bridge between invariant Cys(69) and Cys(238)

112 r compatibility with collagen identifies the disulfide bridge between proximal homocysteine (Hcy) and

113 The peptide contains a disulfide bridge between residues two and seven and an a

114 The processing sites and a disulfide bridge between the 18- and 10-kDa chains could

115 on, whereas the chemokine receptor conserved disulfide bridge between the N terminus and TMVII is nee

116 (ECL)-2, chemokine receptors (CCR) contain a disulfide bridge between the N terminus and what previou

117 more stable version of cAb-HuL22 by adding a disulfide bridge between the two beta-sheets in the hydr

118 The disulfide bridge between the two chromophores is cleaved

119 the 7 transmembrane receptor (7TM)-conserved disulfide bridge between transmembrane (TM) helix 3 and

120 The protein also contained an intramolecular disulfide bridge between two cysteines (Cys) that are co

121 eine residue (Cys-298) in position to form a disulfide bridge between two Siglec-E polypeptides.

122 CHCH-domain containing subunits that contain disulfide bridges between CX9C motifs; they are processe

123 odimers and heterodimers with E1 mediated by disulfide bridges between cysteine residues.

124 activity through the reversible formation of disulfide bridges between cysteine thiols.

125 terlinked rings A and B are obtained through disulfide bridges between L-Cys3 and D-Cys8 and between

126 triple helix, and the cysteine residues form disulfide bridges between the different strands.

127 forms of an immobilized NGB showed that the disulfide bridge both defines the kinetics of NO dioxyge

128 t all of the cysteine residues known to form disulfide bridges both within each monomer and between m

129 used on an antigenic peptide fragment from a disulfide bridge-bounded region spanning the V1 and V2 h

130 Because of this, ZmTrxh is unable to reduce disulfide bridges but possesses a strong molecular chape

131 It revealed the presence of four disulfide bridges, but no close structural analogs could

132 eolysis and to reduction of its intersubunit disulfide bridges by glutathione.

133 the helix-loop-helix fold stabilized by two disulfide bridges C(1)-C(4) and C(2)-C(3).

134 Moreover, the intramolecular disulfide bridge (C23/45) of HMGB1 is required for bindi

135 deposited poly(allylmercaptan) surfaces via disulfide bridge chemistry and are found to readily unde

136 , state-selective molecular stapling through disulfide bridges, competition binding saturation transf

137 ve applied electrochemistry (EC) to overcome disulfide bridge complexity in top-down analysis of mAbs

138 Engineered disulfide bridges, computationally predicted to interfer

139 se of the LC from the HC by reduction of the disulfide bridge concomitant with LC refolding in the cy

140 In the conventional pattern, two disulfide bridges connect an alpha-helical segment to th

141 ach for such mapping and apply it to a three-disulfide-bridged conotoxin, mu-SxIIIA (from the venom o

142 y selectivity and metabolic stability of the disulfide-bridge-containing peptide 1.

143 Here, we have used disulfide bridge cross-linking to test this hypothesis.

144 Disulfide bridge crosslinking demonstrates that SecA tra

145 Using disulfide bridge crosslinking, we have stabilized the E.

146 A disulfide-bridged cyclic RGD peptide, named iRGD (intern

147 amics and self-association by disrupting the disulfide bridge (Cys(3)-Cys(14)) in SST.

148 ints, including interhelical H-bonds and two disulfide bridges (Cys(40)-Cys(279), Cys(271)-Cys(277))

149 Intersubunit disulfide bridges, Cys-206-Cys-206 and Cys-243-Cys-243,

150 in each case contains the canonical A20-B19 disulfide bridge (cystines 18-61 in IGF-I and 19-85 in h

151 of C69G-GES-5 shows that two domains of this disulfide bridge-deficient enzyme are held together by a

152 contrary to a recent report, human ALR is a disulfide-bridged dimer (linked via C15-C124) with two f

153 Rigidifying this contact permanently with a disulfide bridge disrupts ligand-induced receptor activa

154 The structure reveals a five-disulfide-bridged domain similar to CRDs of Frizzled pro

155 We propose that the formation of disulfide bridges drives membrane insertion of FGF2 olig

156 ircuit current were reduced, only one of the disulfide bridges eliminated the activity of the toxins

157 re, the rigid nature of this modification by disulfide bridging enables the successful detection of a

158 se ligand-binding clefts were locked shut by disulfide bridges engineered across lobes.

159 predicted by molecular modeling to disrupt a disulfide bridge essential for the proper folding of the

160 Additionally, interchain disulfide bridge experiments showed that the relative or

161 t suggest that the enzyme does not require a disulfide bridge for its activity as suggested elsewhere

162 on, partial oligomerization was observed via disulfide bridge formation at cysteine 482 in close prox

163 stability of the PLN pentameric assembly via disulfide bridge formation, preventing its binding to Ca

164 itated synthesis, purification, and directed disulfide bridge formation.

165 he human protein predicted to participate in disulfide bridge formation.

166 Disulfide bridges formed at C349/C356 and C465/C468 of t

167 Disulfide bridges formed between C322 residues of differ

168 disulfide connectivity in peptides with many disulfide bridges has proven to be laborious and general

169 characterized by exclusively intermolecular disulfide bridges have been analyzed by molecular modeli

170 Similarly, Cys-Cys disulfide bridges have been used to impose specific asso

171 This suggests a structural role of the disulfide bridges helping to mold the high-affinity doma

172 d the modification to the peptide-containing disulfide-bridged hinge structure.

173 f the protein with reduced three out of four disulfide bridges), human insulin, bovine core histones,

174 ncated 34 amino acid form consisting of four disulfide bridges identified that AGRP contains an inhib

175 In addition to the canonical internal disulfide bridge, Ig1 contains a second, solvent-exposed

176 the C-terminus that does not interfere with disulfide bridges, (ii) does not require activation, and

177 onomer in redox communication with an active disulfide bridge in a variant of the fold adopted by NTR

178 stigate the role of an intermittent internal disulfide bridge in determining NO oxidation kinetics at

179 Here, we investigate the role of the disulfide bridge in the dynamics of protein translocatio

180 Here we show that disruption of the disulfide bridge in the GES-5 carbapenemase by the C69G

181 solution has been activated by breaking the disulfide bridge in the triad Trp/Cys-Cys through absorp

182 ive, and as a consequence, disruption of the disulfide bridge in these enzymes destabilizes them, whi

183 ind that as much as 18% of all proteins with disulfide bridges in a non-redundant subset of PDB form

184 The results highlight the importance of disulfide bridges in a small bioactive peptide to bring

185 tion, resulting in formation of adventitious disulfide bridges in cell proteins.

186 aa in length and have one to three predicted disulfide bridges in each half.

187 Furthermore, we observed that disulfide bridges in eotaxin, epidermal growth factor, a

188 Surprisingly, two of the three disulfide bridges in IL-12alpha are dispensable for IL-1

189 tive cross-links was demonstrated by mapping disulfide bridges in RcsF, an outer membrane lipoprotein

190 tive disulfide bridge, which further reduces disulfide bridges in target proteins to regulate their s

191 protein is 132 amino acids and contains five disulfide bridges in the C-terminal domain.

192 We also identified two highly conserved disulfide bridges in the Cdc50 ectodomain.

193 that the successive reduction of one or both disulfide bridges in the dimer results in a stepwise dec

194 Four disulfide bridges in the extracellular domain, combined

195 teine residues generated by reduction of the disulfide bridges in the hinge region or surface lysine

196 e capacity of Cys-306 to form intermonomeric disulfide bridges in the presence of an oxidizing agent,

197 In addition, evaluation of disulfide bridging in native membranes suggests alterati

198 at position 71 was involved in interprotomer disulfide-bridging in TraM, altering Cys-71 to a serine

199 s at the C terminus and the fourth and fifth disulfide bridges, inhibited FXIIa with a Ki of 116 +/-

200 t quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with th

201 dox reaction with copper ions resulting in a disulfide bridge involving Cys466.

202 ctural features of the protein, particularly disulfide bridges involving CXC motifs in the extracellu

203 nnels, which form oligomers held together by disulfide bridges involving Cys-73, accumulated in the p

204 Thus, either APP palmitoylation or disulfide bridges involving these Cys residues appear to

205 Here we show that the intramolecular disulfide bridge is constructed from the acyclic ene-dit

206 Our results demonstrate that the disulfide bridge is essential for stability but does not

207 The disulfide bridge is essential in maintaining the structu

208 tide linkage between LC and HC in place of a disulfide bridge is insufficient for productive LC trans

209 teine residues revealed that the Cys22-Cys28 disulfide bridge is not required for cleavage.

210 It was proposed that this conserved disulfide bridge is responsible for their carbapenemase

211 ogues of ProTx-II, in which one of the three disulfide bridges is replaced with a thioether linkage,

212 A defined arrangement of these disulfide bridges is unique to each isoform.

213 Cross-linking subunits through disulfide bridging is commonly used to stabilize multime

214 IIA (KIIIA), a 16-residue peptide with three disulfide bridges, is a pore blocker of voltage-gated so

215 The presence of the disulfide bridge isolates the active site from solvent a

216 d sheds light on the molecular processes and disulfide bridges isomerization underlying the conformat

217 examined leads to the formation of different disulfide-bridged isomers indicating the requirement of

218 These disulfide bridges link residue pairs > 40 A apart in the

219 ver medium- to long-range distances across a disulfide bridge linking loops L1 and L2, which constitu

220 how the possibility of previously unreported disulfide bridges linking the M1 and M3 transmembrane he

221 ino acids and the presence of three internal disulfide bridges may hamper its development for in vitr

222 rogate NS5A-NS5A interactions, implying that disulfide bridges may play a role in this interaction.

223 he C terminus, and two pairs of intrasubunit disulfide bridges may play an important role in its ther

224 The disulfide bridge must be on the trans compartment to ach

225 We show that the disulfide bridge must remain intact throughout LC transl

226 a 35-amino acid peptide cross-linked by two disulfide bridges named tau-AnmTX Ms 9a-1 (short name Ms

227 alpha-Conotoxins are two-disulfide-bridged nicotinic antagonists, 13-19 amino aci

228 Analysis of the disulfide bridges of a natural peptide tx3c from C. text

229 proposes (i) to reduce by ETD one of the two disulfide bridges of model peptides, resulting in the op

230 We show that the disulfide bridges of RTD-1, a member of the theta-defens

231 , a deletion in this domain restructures the disulfide bridges of this domain, resulting in a pseudok

232 ereochemistry and the number of atoms in the disulfide bridge on binding affinity.

233 y thioredoxin-mediated redox modulation of a disulfide bridge on its gamma subunit.

234 own to be regulated by redox modulation of a disulfide bridge on the gamma-subunit through the ferred

235 AtSLP2 and is dependent on the formation of disulfide bridges on AtSLP2.

236 Here, we investigate the influence of disulfide bridges on protein dynamics through molecular

237 ohydrates for protein screening using either disulfide bridge or Schiff base imine immobilization che

238 Lassos arise in proteins with disulfide bridges (or in proteins with amide linkages),

239 Reduction of disulfide bridges partially restores activity.

240 ticularly in the conservation of distinctive disulfide bridge patterns.

241 s of other hydrophobic peptides with complex disulfide bridge patterns.

242 We have also investigated the folding and disulfide bridging patterns arising from different metho

243 It is a 17-amino-acid, two-disulfide bridge peptide.

244 We isolated and identified a disulfide-bridged peptide (+ICDINGVCVDA-), termed incept

245 facilitates the detailed characterization of disulfide-bridged peptides by mass spectrometry.

246 these two methodologies, by starting with a disulfide bridged phage display peptide which binds a hu

247 Disulfide bridges play a major role in defining the stru

248 hydrophobic loop to the G-actin surface by a disulfide bridge prevents filament formation.

249 uently results in incomplete dissociation of disulfide bridges prior to MS analysis, leading to a los

250 ucers results in formation of intermolecular disulfide bridges, probably between C273 of one Keap1 mo

251 ctroscopy and the structure of an engineered disulfide-bridged Psu derivative reveal that the protein

252 lysis of cysteine mutants that disrupt these disulfide bridges revealed an inverse relationship betwe

253 oint mutants that engineer extra interdomain disulfide bridges rigidify the UGGT structure and exhibi

254 consistent with the replacement of up to two disulfide bridges (S-S) with a like number of trisulfide

255 he inhibitory helix onto the ETS domain by a disulfide bridge severely impairs, but does not abolish

256 accessibility, disordered regions, domains, disulfide bridges, single mutation stability, residue co

257 ngal metabolite gliotoxin has a redox-active disulfide bridge spanning carbons 3 and 6 of a diketopip

258 The disulfide bridges stabilize the tertiary structure of th

259 nal SDS-PAGE, we detected CL-K1 and CL-L1 in disulfide bridge-stabilized complexes.

260 twork and by comparison to the corresponding disulfide-bridged structure.

261 bility of an unprecedented helix-terminating disulfide bridge that could alter COX/cytochrome c disso

262 tations at these sites and found they formed disulfide bridges that decreased the channel open dwell

263 enom peptides from cone snails with multiple disulfide bridges that provide a rigid structural scaffo

264 ified the number of inter- or intramolecular disulfide bridges, the number of Cu(I) or Cu(II) ions, t

265 FI is composed of two chains linked by a disulfide bridge; the light chain comprises only the ser

266 observed in minor abundance (with respect to disulfide bridged tiopronin species) before dramatically

267 onent of a low-density lipoprotein through a disulfide bridge to form lipoprotein(a).

268 ubunit encoded by an Abpa gene and linked by disulfide bridges to an ABPBG subunit encoded by an Abpb

269 paramyxovirus inhibitors, we have engineered disulfide bridges to introduce covalent links into the p

270 Nature uses interchain disulfide bridges to stabilize collagen trimers.

271 ompartments generally rely on intramolecular disulfide bridging to maintain conformation (e.g., album

272 proposed, together with the beta-turn and a disulfide bridge, to be essential for binding to the tar

273 ort here the design and synthesis of HP-1, a disulfide-bridged two-alpha-helix peptide that self-asse

274 sidues were exchanged with one another and a disulfide-bridged variant (H281C/S524C) where the two re

275 The disulfide-bridged variant showed approximately 20% incre

276 In the infected cells, p6 forms a disulfide bridge via a cysteine residue located near the

277 tionalized 1,4-bisthiophenol units linked by disulfide bridges was obtained by self-assembly on a gra

278 conditions, Ltn variants containing a second disulfide bridge were designed.

279 Phe-43) from the L3,4 loop and a distinctive disulfide bridge were shown to account for the high affi

280 he analysis of a previous work in which four disulfide bridges were constructed in domain I of the Cr

281 nanoparticle stability, artificial covalent disulfide bridges were introduced throughout the VLP.

282 bolished binding of Fab 1A5, indicating that disulfide bridges were required for the structural integ

283 y of dendrimers displaying internally queued disulfide bridges were synthesized and exploited as flaw

284 However, pilins have disulfide bridges, whereas the major pseudopilins of T2S

285 ucing equivalents from FAD to a redox-active disulfide bridge, which further reduces disulfide bridge

286 The data indicate further that there is a disulfide bridge, which is not required for function, be

287 idge, Ig1 contains a second, solvent-exposed disulfide bridge, which our biochemical data indicate is

288 hereas the other eight cysteines formed four disulfide bridges, which included the first (Cys(73)) an

289 The catalytic cysteine needs to form a disulfide bridge with a conserved cysteine, which is sub

290 ver it to the Cys residues of IscU, formed a disulfide bridge with Fdx in the presence of an oxidizin

291 ular domain of ENaC and likely involved in a disulfide bridge with the partner cysteine C394.

292 o cysteine (V51C) to make a symmetry-related disulfide bridge with the preexisting Cys 53 on the oppo

293 s may be unpaired and could therefore form a disulfide bridge with VCP.

294 aging through oxidation of proteins, forming disulfide bridges with cysteine or methionine sulfhydryl

295 otoxin contains eight cysteines forming four disulfide bridges with sequence similarities resembling

296 e 29 amino acid peptides cross-linked by two disulfide bridges, with a primary structure similar to o

297 method is demonstrated by partial mapping of disulfide bridges within a 37-kDa protein containing 16

298 PDI catalyzes an isomerization of disulfide bridges within the thioredoxin motif C600XXC60

299 can be attached to protein surfaces via two disulfide bridges, yielding a probe that is rigid relati

300 the introduction of appropriately positioned disulfide bridges yields a force resistant complex with