ライフサイエンスコーパス: holdfast

1 n adhesive polar polysaccharide known as the holdfast.

2 an extremely strong polar adhesin called the holdfast.

3 rosilicate substrates through their adhesive holdfast.

4 cells with their stalks attached to the same holdfast.

5 t, we determine the elastic stiffness of the holdfast.

6 orm between the stalks as they make a shared holdfast.

7 lay an important role in the strength of the holdfast.

8 , consistent with the gel-like nature of the holdfast.

9 y can be attributed to the elasticity of the holdfast.

10 th the polysaccharide gel-like nature of the holdfast.

11 tent with the elastic characteristics of the holdfast.

12 y attached to a surface through its adhesive holdfast.

13 drag structures generated by uprooted frond holdfasts.

14 cs the wet adhesive proteins found in mussel holdfasts.

15 ter crescentus attachment is mediated by the holdfast, a complex of polysaccharide anchored to the ce

16 The holdfast, a complex polysaccharide composed in part of N

17 rotation and concurrent stimulation of polar holdfast adhesive polysaccharide.

18 The tip of the stalk is decorated with a holdfast, an adhesive organelle composed at least in par

19 eDNA binds to the polar holdfast, an adhesive structure required for permanent s

20 re randomly positioned and colocalize with a holdfast anchor protein in these strains, indicating tha

21 lar organelles, including the pili, adhesive holdfast and chemotactic apparatus, by recruiting struct

22 (flgH) mutants, and a double mutant lacking holdfast and flagellum (hfsA; flgH), a model for biofilm

23 s required for the synthesis of the adhesive holdfast and pili.

24 elements), the adhesion strength between the holdfast and the substrate is >68 N/mm(2) in the central

25 lar asymmetry that leads to the synthesis of holdfasts and pili at their proper subcellular location.

26 olar organelle synthesis, including pili and holdfast, and flagellum ejection, is mediated in part by

27 is anchored into the sea floor by a flexible holdfast apparatus consisting of thousands of anchor spi

28 Restored holdfasts are randomly positioned and colocalize with a

29 r, like the A. biprosthecum hfsH mutant, the holdfasts are shed into the medium and have decreased ad

30 attachment is mediated by the synthesis of a holdfast as the swarmer cell differentiates into a stalk

31 We model the holdfast as three torsional springs in series and find t

32 the adhesive and cohesive properties of the holdfast, as well as for the anchoring of the holdfast t

33 Therefore, the force constant of the stalk-holdfast assembly can be attributed to the elasticity of

34 ic properties of the C. crescentus stalk and holdfast assembly were studied by using video light micr

35 to determine the force constant of the stalk-holdfast assembly, which quantifies its elastic properti

36 that the hfsDAB mutants fail to synthesize a holdfast at the stalk tip.

37 mutant, suggesting that HfaB is involved in holdfast attachment beyond secretion of HfaA and HfaD.

38 e cell, we have examined the regulation of a holdfast attachment gene, hfaA.

39 r development genes (podJ and pleC), and the holdfast attachment genes (hfa).

40 hfaC was not required for holdfast attachment or binding to surfaces.

41 uch more severely deficient in adherence and holdfast attachment than hfaA and hfaD mutants.

42 ative contribution of the different genes to holdfast attachment, mutations were constructed for each

43 For lasting holdfast attachment, the mussel Mytilus californianus co

44 an uncharacterized gene cluster involved in holdfast biogenesis (hfs) as well as in previously ident

45 s gene cluster (hfsDAB) resulted in a severe holdfast biogenesis phenotype.

46 HfsB is a novel protein involved in holdfast biogenesis.

47 ed in the bacterial cell (e.g. type IV pili, holdfasts, chemoreceptors), but perhaps none show so man

48 mately one-third of that of a wet (in water) holdfast, consistent with the gel-like nature of the hol

49 The holdfast deficiency of hfsB and podJ mutants is suppress

50 pecifically to polar polysaccharides, termed holdfast, discriminated irreversible adhesion events fro

51 Polar localization of the holdfast export protein, HfsD, depends on the presence o

52 that the hfs genes play an important role in holdfast export.

53 Indirect evidence suggested that the holdfast first appears at the swarmer pole of the prediv

54 h that PleC localization is not required for holdfast formation and motility in soft agar.

55 uction and a cytoplasmic region required for holdfast formation and swarming motility, and establish

56 nts from reversible adhesion events where no holdfast formed.

57 us is mediated by an adhesive organelle, the holdfast, found at the tip of the stalk.

58 C. crescentus strain CB15 wild type and its holdfast (hfsA; DeltaCC0095), pili (DeltapilA-cpaF::Omeg

59 centus revealed that this strain synthesizes holdfast; however, like the A. biprosthecum hfsH mutant,

60 ansmission electron microscopy to detect the holdfast in different cell types.

61 a major component of the byssus, an adhesive holdfast in mussels.

62 visional cells, we were unable to detect the holdfast in swarmer cells or at the flagellated poles of

63 haride deacetylase, leads to accumulation of holdfast in the culture supernatant.

64 f the holdfast, resulting in the presence of holdfasts in motile daughter cells.

65 ndicates that the height of a dried (in air) holdfast is approximately one-third of that of a wet (in

66 The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylgl

67 We show that the holdfast is essential but not sufficient for optimal att

68 effective torsional spring constant for the holdfast is of the order of (10(-17)-10(-18)) Nm, with u

69 ytilus californianus owe their tenacity to a holdfast known as the byssus, a fibrous extracellular st

70 taches to solid surfaces through an adhesive holdfast located at the tip of its polar stalk, a thin c

71 The hfaA and hfaD mutants shed some holdfast material into the surrounding medium and were p

72 The synthesis of the stalk and holdfast occur at the same pole during swarmer cell diff

73 podJ null mutants are unable to form holdfast or pili, have reduced swarming motility, and ha

74 ls have a remarkable ability to attach their holdfast, or byssus, opportunistically to a variety of s

75 The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfas

76 sis genes (hfsEFGH) directly adjacent to the holdfast polysaccharide export genes.

77 Hfa protein localization requires the holdfast polysaccharide secretion proteins and the polar

78 ar weight (HMW) form requiring HfaD, but not holdfast polysaccharide.

79 faD HMW form is dependent on HfaA but not on holdfast polysaccharide.

80 osynthesis of the minimum repeat unit of the holdfast polysaccharide.

81 In wild-type cells, the holdfast production time for irreversible adhesion event

82 contact (23 s) was 30-times faster than the holdfast production time that occurs through development

83 wet conditions, as occurs in self-assembled holdfast proteins in mussels and other marine organisms,

84 ich coincided with synthesis of the adhesive holdfast required for attachment.

85 ns lack the cell-specific segregation of the holdfast, resulting in the presence of holdfasts in moti

86 ated that the hfaB mutants had the strongest holdfast shedding phenotype.

87 th in surface adhesion and in binding to the holdfast-specific lectin wheat germ agglutinin.

88 For both biofilm forms, the holdfast structure at the tip of a stalked cell is cruci

89 characterization of proteins extracted from holdfast structures produced by these organisms.

90 To investigate the timing of holdfast synthesis and exposure to the outside of the ce

91 Restoration of holdfast synthesis at non-polar sites reduces surface ad

92 t with the need to spatially co-ordinate the holdfast synthesis machinery with the flagellum and pili

93 etraction, which was sufficient to stimulate holdfast synthesis without surface contact.

94 increased cell adherence without increasing holdfast synthesis.

95 sses a concentration threshold necessary for holdfast synthesis.

96 fs genes, are absolutely required for proper holdfast synthesis.

97 ited nearly wild-type levels of adhesion and holdfast synthesis.

98 hfsE, two of which are redundant to hfsE for holdfast synthesis.

99 oldfast, as well as for the anchoring of the holdfast to the cell envelope.

100 fast polysaccharide and the anchoring of the holdfast to the cell were previously discovered.

101 This suggests that exposure of the holdfast to the outside of the cell occurs during the di

102 train CB2A and involved in attachment of the holdfast to the polar region of the cell.

103 three proteins form a complex anchoring the holdfast to the stalk.

104 been shown to disrupt the attachment of the holdfast to the tip of the stalk, but the role of indivi

105 HfaD, and probably HfaA, serve to anchor the holdfast to the tip of the stalk.

106 tylglucosamine (GlcNAc) to the elasticity of holdfast was examined by lysozyme digestion.

107 While the holdfast was readily detectable in stalked cells and at

108 gle for a pair of cells attached to a single holdfast, we determine the elastic stiffness of the hold

109 ciate tightly with the biofilm through their holdfast, we hypothesize that this novel mechanism acts

110 stalk is an adhesive organelle known as the holdfast, which the stalked cell uses to attach to a sol

111 is mediated by a polar organelle called the "holdfast," which enables the bacterium to form stable mo