ライフサイエンスコーパス: invertase

1 on (40-50%) in release of a secreted enzyme (invertase).

2 ters and a putative beta-fructofuranosidase (invertase).

3 lation of SbVIN1, a gene encoding a vacuolar invertase.

4 and the high mannose-containing glycoprotein invertase.

5 unoisolated clathrin-coated vesicles contain invertase.

6 r the endodermis and phloem for soluble acid invertase.

7 a suitable support for the immobilisation of invertase.

8 Pex15p was fused to the N-terminus of mature invertase.

9 lix that controls these steps by the Hin DNA invertase.

10 da repressor protein to the secreted protein invertase.

11 nts are shuffled by Rci, a site-specific DNA invertase.

12 reakdown through sucrose synthase instead of invertase.

13 ished their studies of sucrose hydrolysis by invertase.

14 fect on the retained activity of immobilized invertase.

15 aride must first be hydrolysed by the enzyme invertase.

16 d prokaryotic serine site-specific resolvase-invertases.

17 terminal domains compared with the resolvase/invertases.

18 rved for the differentially sugar-responsive invertases.

19 o each other and the FimE and HbiF bacterial invertases.

20 nthetic genes may have evolved from vacuolar invertases.

21 s encoding a SWEET transporter and cell wall invertases.

22 Upon rapid induction of yeast cytosolic invertase, a marked phenotype appears in developing leav

23 nthase activity and reduced Suc synthase and invertase activities, leading to increased Suc contents.

24 Despite a considerable rise in invertase activity (200 units) during ripening of Shahan

25 ted well with an asymmetric increase in acid invertase activity across the pulvinus.

26 Both invertase activity and (1)(3)C import were greater in sh

27 In the transformed plants, invertase activity and a 70-kD cross-reacting protein we

28 loem girdling on (1)(3)C and (1)(5)N import, invertase activity and polyphenol accumulation in juveni

29 This corresponded to an increase in invertase activity and the accumulation of phenolic comp

30 enile tissues was positively correlated with invertase activity at the treatment site and enhanced by

31 Analysis of invertase activity in nontuberizing and tuberizing stolo

32 carrot lines revealed very low acid-soluble invertase activity in rs/rs roots whereas neutral invert

33 Invertase activity is reduced in vitro in the presence o

34 Invertase activity is thought to play a regulatory role

35 Variation in acid invertase activity reflects multiple evolutionary events

36 However, cell wall-bound invertase activity remained high in the apical 1 to 2 mm

37 orms a boundary, compartmentalizing apoplast invertase activity to allow different embryo and endospe

38 Changes in endosperm invertase activity were complex and quantitatively do no

39 ected in significant reductions in cell wall invertase activity.

40 ross-reacting polypeptide, but was devoid of invertase activity.

41 ing both Asp-239 and Trp-47 homologs, has no invertase activity.

42 70% of the sugars consumed were released by invertase activity.

43 ucrose and starch hydrolysis (e.g. cell wall invertase, alpha-amylase, and starch phosphorylase) were

44 Invertase, an enzyme responsible for sucrose metabolism,

45 invertase) of the signal toward its analyte invertase and a negligible nonspecific interaction of th

46 The activities of invertase and cell wall-modifying enzymes, namely pectin

47 r carboxypeptidase Y (CPY), accumulates both invertase and CPY in dense vesicles.

48 Despite evidence that yeast invertase and dry heat degrade inulin, the extent to whi

49 identically to wild type and show delays of invertase and Gas1p ER-to-Golgi transport identical to t

50 e Y trafficking defect, but the secretion of invertase and gp400/hsp150 is not significantly affected

51 temperature; however, other proteins (i.e., invertase and HSP150) in these and other COPI mutants we

52 ulate an internal pool of fully glycosylated invertase and mature alpha-factor, while processing and

53 ycoside hygromycin B, and exhibit diminished invertase and Sim1 glycosylation.

54 elongation intermediates were released from invertase and similarly analyzed.

55 ptide followed by the coding region of yeast invertase and the transmembrane domain and cytoplasmic t

56 nd rse1-1 mutants accumulate the ER forms of invertase and the vacuolar protease CPY at restrictive t

57 ular C terminus can be processed to secreted invertase and this fraction is constrained to 2-3% by a

58 ncodes an integrase related to the resolvase/invertases and is evolutionarily and mechanistically dis

59 apid change in the regulation and balance of invertases and Suc synthases that could have an immediat

60 inding domain of the Hin family of bacterial invertases and to homeodomain proteins.

61 ersatile and include integrases, resolvases, invertases and transposases.

62 ndent fashion, the transcription of vacuolar invertase, and a wak2 mutant alters the normal pectin re

63 y owing to a jasmonic acid-induced cell wall invertase, and is limited by phloem sucrose availability

64 ferent sets of cargoes typified by Bgl2p and invertase are delivered to the plasma membrane for secre

65 The archetypal resolvase/invertases are highly regulated, only affect resolution

66 Invertases are regulated at the posttranslational level

67 biological role has been associated with the invertase as of yet.

68 stained by both sucrose synthase and neutral invertase, associated with minimal futile cycling.

69 i forms of both CPY and the secreted protein invertase at the nonpermissive temperature.

70 a is involved in degradation of the vacuolar invertase AtFruct4 in aging tissues.

71 The Hin DNA invertase becomes catalytically activated when assembled

72 invertase-repressor fusions, like wild-type invertase, behave as soluble proteins in the ER lumen.

73 inetics was based on the properties of yeast invertase (beta-fructo-furanosidase, EC 3.2.1.26), whose

74 Invertase (beta-fructosidase, EC 3.2.1.26) hydrolyzes su

75 l studied serine recombinases, the resolvase/invertases, bring two recombination sites together in a

76 stingly, CPY-invertase hybrid proteins, like invertase but unlike CPY, escaped the sec21 ts mutant ER

77 group, are similar in size to the resolvase/invertases but have the DNA binding domain N-terminal to

78 ing an Och1p-invertase fusion do not secrete invertase, but those expressing an Och1p-Kex2p site-inve

79 e at about a 2-fold molar excess inactivated invertase by modifying both of the enzyme's cysteine res

80 Rapid repression of the Ivr1 and Ivr2 maize invertases by low oxygen was evident in root tips within

81 ity with allelic variants within an alkaline invertase candidate gene LpcAI.

82 on of DNA in the sample into glucose through invertase-catalyzed hydrolysis of sucrose.

83 It is proposed that an activated invertase causes the immediate loss of the plasmid in mo

84 ut activities of neutral invertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, a

85 , released from wheat fructan and sucrose by invertase, compared to maltose is, however, not document

86 is based on target-dependent binding of cDNA-invertase conjugate with the analyte DNA, thereby transf

87 d release of invertase from a functional-DNA-invertase conjugate.

88 In both assay methods, with invertase conjugates as the link, quantitative detection

89 These data suggest that soluble acid invertase controls sugar composition in tomato fruit and

90 The released invertase converts sucrose into glucose, which is detect

91 Cell wall invertases (cwINVs), with a high affinity for the cell w

92 forming the resulting fusion library into an invertase-deficient yeast strain and plating the transfo

93 esults demonstrate that unique resolvase and invertase derivatives can be developed to site-specifica

94 ino acid residues that mediate resolvase and invertase DNA sequence specificity.

95 evealed the molecular basis of resolvase and invertase DNA sequence specificity.

96 low temperature, defects in glycosylation of invertase, dominant lethality, fluoride sensitivity, and

97 thase in the embryo was greater than that of invertase during development.

98 that ZM-INVINH1 interacts with an apoplastic invertase during early kernel development.

99 ic isotope fractionation associated with the invertase (EC 3.2.1.26) and glucose isomerase (EC 5.3.1.

100 A-3) was used for covalent immobilization of invertase (EC 3.2.1.26).

101 We show here that a cell-wall invertase encoded by the Incw1 gene is regulated at both

102 of phage integrases related to the resolvase/invertase enzymes.

103 Man10GlcNAc and Man11GlcNAc species from invertase expressed in Pichia pastoris showed three and

104 Finally, we note that glucose repression of invertase expression in wild-type cells produces a strat

105 An association of invertase expression with generative tissue, both in veg

106 ugh incomplete cell separation, 10 increased invertase expression, none imported sucrose, and 11 incr

107 ng sucrose before hydrolysis, and increasing invertase expression.

108 Invertase family members reside on segmental duplication

109 catalytic domain derived from the resolvase/invertase family of serine recombinases and a custom-des

110 Hyperactivated variants of the resolvase/invertase family of serine recombinases function without

111 catalytic domains derived from the resolvase/invertase family of serine recombinases fused to Cys2-Hi

112 Members of the resolvase/invertase family of site-specific recombinases require s

113 gulatory divergence is characteristic of the invertase family.

114 ily and the serine recombinases or resolvase/invertase family.

115 These DNA invertases flip promoter regions in their immediate down

116 od is based on the target-induced release of invertase from a functional-DNA-invertase conjugate.

117 When applied to a sample of invertase from Aspergillus nidulans, the method indicate

118 Piv, a site-specific invertase from Moraxella lacunata, exhibits amino acid h

119 ion and characterization of an intracellular invertase from Trichoderma virens (TvInv) important for

120 es and involves at least three conserved DNA invertases from two evolutionarily distinct families.

121 vertase, vacuolar invertase, cell wall-bound invertase, fructose kinase, and hexokinase were unaffect

122 Cells expressing an Och1p-invertase fusion do not secrete invertase, but those exp

123 The Och1p-Kex2p site-invertase fusion protein is cleaved with a half-time of

124 se, but those expressing an Och1p-Kex2p site-invertase fusion protein secrete high levels of invertas

125 ll surface protein can synthesize a secreted invertase fusion protein that can rescue the mutant, and

126 ansformed with the promoter of an apoplastic invertase gene (invGE) linked to a reporter gene also re

127 1, 2 and 3) showed that sequences for maize invertase gene and for events MON810 and TC1507 were eas

128 We show here that invertase gene expression and the invertase-sucrose (Suc

129 tional processing was observed during normal invertase gene expression in potato.

130 ic fruit expressing a constitutive antisense invertase gene had increased sucrose and decreased hexos

131 no effect on the expression of the cell wall invertase gene in fluorescent carrot cells containing ar

132 cDNA cloning vector which carries a modified invertase gene lacking its leader sequence is used in co

133 rate that silencing the potato vacuolar acid invertase gene VInv prevents reducing sugar accumulation

134 es was located adjacent to the 5' end of the invertase gene, inv.

135 ealed asymmetric induction of one maize acid invertase gene, Ivr2, consistent with transcriptional re

136 omyces cerevisiae deleted for its endogenous invertase gene.

137 m the phloem to the apoplast where cell wall invertases generate monosaccharides for uptake and utili

138 The organisation of two invertase genes (invGE and invGF) linked in direct tande

139 ed together with multiple copies of vacuolar invertase genes and a transposable element on two barley

140 We show that two invertase genes in potato, like most other plant inverta

141 rtase genes in potato, like most other plant invertase genes, include a very short second exon of 9 b

142 In potato invertase genes, the constitutively included, 9-nucleoti

143 hich differs from previously described plant invertase genes; while intron locations are conserved be

144 hyperactivated catalytic domain from the DNA invertase Gin and an optimized TALE architecture.

145 mming of the DNA sequence specificity of the invertase Gin from bacteriophage Mu and Tn3 resolvase fr

146 ed activated mutant (M114V) of the G-segment invertase (Gin) in which one dimer half is rotated by 26

147 zyme sequential reaction was performed using invertase, GOX, and HRP.

148 lta gal83Delta strain is unable to derepress invertase, grows poorly on alternative carbon sources an

149 This yeast invertase had plant-specific complex glycans, indicating

150 Using the protein invertase, Hardklor identifies 18O-labeled peptide isoto

151 genic plants with altered extracellular acid invertase have highly disturbed growth habits.

152 Interestingly, CPY-invertase hybrid proteins, like invertase but unlike CPY

153 ys showing the highest enzyme activity after invertase immobilisation.

154 ertase fusion protein secrete high levels of invertase in a Kex2p-dependent manner.

155 gated the role of intracellular soluble acid invertase in plant and fruit development.

156 ions also had greatly reduced levels of acid invertase in ripe fruit.

157 Transgenic plants were generated with yeast invertase in the cell walls to prevent Suc loading by th

158 Inactivation of the invertase in the donor strain resulted in a 1,000-fold i

159 stolons revealed a marked decline in soluble invertase in the subapical region of swelling stolons, c

160 the expression of a membrane-anchored yeast invertase in transgenic plants.

161 itation led to a down-regulation of vacuolar invertases in all plants, which resulted in an augmentat

162 disruption of sucrose cleavage by cell wall invertases in developing ovaries.

163 ic analysis of the fructosyltransferases and invertases in the Poaceae showed that the fructan biosyn

164 belongs to a small gene family of apoplastic invertases in tomato (Lycopersicon esculentum), is a qua

165 For grape invertase, in parallel with deglycosylated peptides anal

166 which includes transposon resolvases and DNA invertases, in that they utilize two simple but differen

167 Miniature1 (Mn1) locus encodes the cell wall invertase INCW2, which is localized predominantly in the

168 on at the Mn1 locus that encodes a cell wall invertase (INCW2) that localizes exclusively to the basa

169 ed in a 3, 000-fold reduction in the kcat of invertase indicating that Glu-204 plays a major role in

170 We found that in maize (Zea mays), an invertase inhibitor homolog (ZM-INVINH1) is expressed ea

171 nsported by dense exocytic vesicles, such as invertase, into light exocytic vesicles, whereas transpo

172 sucrose in cells in which the only source of invertase is a C-terminal fusion to a transmembrane prot

173 Vacuolar invertase is a key enzyme of sugar metabolism in grape b

174 mutant cheater strain that does not produce invertase is able to take advantage of and invade a popu

175 Invertase is an enzyme that is widely distributed among

176 ged fusion proteins was constructed in which invertase is appended to the Golgi-luminal carboxy termi

177 roteomic approach demonstrates that vacuolar invertase is glycosylated at all twelve potential N-glyc

178 This DNA invertase is necessary for the inversion of at least 13

179 f RAG1 with sequence similarity to bacterial invertases is essential for DNA binding.

180 Piv, a unique prokaryotic site-specific DNA invertase, is related to transposases of the insertion e

181 e in Saccharomyces cerevisiae, which encodes invertase, is repressed about 200-fold by high levels of

182 The external invertase isoform 1 (EINV1) was immobilised on eight dif

183 s not occur in the Rs wild-type acid-soluble invertase isozyme II allele, it does occur elsewhere in

184 t intron near the 5' end of the acid soluble invertase isozyme II gene of rs/rs carrots.

185 While the wild-type acid-soluble invertase isozyme II transcript (ca. 2 kb) was detected

186 idate locus for carrot vacuolar acid-soluble invertase isozyme II.

187 Increased invertase levels in the stressed leaf meristem, on the o

188 44% of the sugars consumed were generated by invertase-mediated degradation of fructan, raffinose and

189 sess glucose flux differences between cells, invertase-mediated sucrose hydrolysis in vivo, delivery

190 Invertase-mediated sugar release seems to be crucial dur

191 TB2 reduced inclusion/splicing of the potato invertase mini-exon splicing reporter, indicating that t

192 This occurs in the potato invertase mini-exon via the polypyrimidine tract and ass

193 a Kex2p cleavage site between the Och1p and invertase moieties to monitor transit to the Kex2p-conta

194 to distal compartments is not induced by the invertase moiety, since noninvertase fusion constructs e

195 In addition to expected genes such as invertases, natural variation was identified in key C4 m

196 ose synthase of soybean and a cell wall acid invertase of carrot.

197 e amino acids of a motif highly conserved in invertases of diverse origin.

198 binase, termed Inv, was highly homologous to invertases of the Din family.

199 Bacterial DNA invertases of the serine site-specific recombinase famil

200 ensor was only 6.1% (probed with an oxidized invertase) of the signal toward its analyte invertase an

201 uces the expression and activity of vacuolar invertase, often a key factor in turgor and expansion.

202 do not complement for Moraxella lacunata Piv invertase or IS492 MooV transposase activities.

203 se entering the fruit can be accomplished by invertase or sucrose synthase.

204 he major pilin subunits (tfpQ/I) and the DNA invertase (piv), which determines pilin type expression.

205 designed and highly convergent resolvase and invertase populations in the context of engineered zinc-

206 ne fetuin, bovine thyroglobulin, and several invertase preparations from wild-type and mutant yeast s

207 n be imported into the cell, serving to make invertase production and secretion a cooperative behavio

208 The Hin DNA invertase promotes a site-specific DNA recombination rea

209 sed appropriately upstream of this defective invertase provide the necessary signals to restore secre

210 In contrast to the resolvase/invertase recombination systems--where there are strict

211 stream partially prevented the abortion, but invertase regulated the synthesis of ovary starch and pa

212 is adjacent to mpi, encoding the global DNA invertase regulating capsular polysaccharide biosynthesi

213 We have named these genes irg for invertase-related gene family.

214 terest to a yeast (Saccharomyces cerevisiae) invertase reporter gene, transforming the resulting fusi

215 (a) The invertase-repressor fusions, like wild-type invertase, b

216 amily of site-specific recombinases--the DNA invertase/resolvase family--that catalyze inversion or d

217 cysteines with alanines revealed that C108A invertase retained full activity whereas C205A was reduc

218 ) released with peptide-N-glycosidase F from invertase secreted by Deltaalg9 yeast showed its structu

219 These cells are defective in invertase secretion and accumulate vesicles similar to t

220 effects of ste24- and spf1-null mutations on invertase secretion are additive, cell generation time i

221 rictive temperature and exhibit a pattern of invertase secretion comparable with sec14(ts) strains.

222 The kinetics of invertase secretion or transport of alkaline phosphatase

223 was found wild type in this mutant, although invertase secretion was impaired.

224 defects in chitin and cell wall deposition, invertase secretion, and fluid phase endocytosis.

225 on of transport vesicles and the decrease in invertase secretion.

226 xtensively glycosylated, indicating that the invertase segment inserted at these Akr1p sites is lumin

227 ity columns, i.e. asialofetuin-Sepharose and invertase-Sepharose.

228 rols, and high-mannose structures from yeast invertase served as negative controls.

229 mportant physiological role of Saccharomyces invertase (SInv) and the historical relevance of this en

230 Hin is a member of the DNA invertase subclass of serine recombinases that are regul

231 Theoretically advantageous reductions in the invertase/Suc synthase balance thus resulted.

232 ere motivated by the potential for a reduced invertase/Suc synthase balance to alter the impact of re

233 Portions of the yeast protein invertase (Suc2p) were inserted in-frame at 10 different

234 tase activity in rs/rs roots whereas neutral invertase, sucrose synthase and sucrose phosphate syntha

235 here that invertase gene expression and the invertase-sucrose (Suc) synthase ratio decrease abruptly

236 we report that FimX, an ExPEC-associated DNA invertase that regulates the major virulence factor type

237 Therefore, in addition to invertase, the growing embryo itself has a potential to

238 ructural groups represented by the resolvase/invertases, the large serine recombinases and relatives

239 Because yeast use invertase to hydrolyze sucrose extracellularly and impor

240 where sucrose is hydrolysed by an apoplasmic invertase to produce a mixture of sucrose, glucose and f

241 own to rapidly encounter the TGN glycosylate invertase to the same extent as wild-type cells, indicat

242 ) plants expressing a constitutive antisense invertase transgene grew identically to wild-type plants

243 sis of a deletion mutant of one of these DNA invertases, tsr15 (aapI), which resulted in the promoter

244 ression is regulated at one level by the DNA invertase Tsr19, which is encoded by a gene immediately

245 By means of sucrose and invertase uptake experiments, we have also shown that ac

246 in silenced fruit, but activities of neutral invertase, vacuolar invertase, cell wall-bound invertase

247 Furthermore, a defect in invertase vesicle trafficking caused by vps1Delta or pep

248 mal amplification method with a thermostable invertase, we can directly transduce Middle-East respira

249 In a previous study on yeast invertase, we identified Asp-23 through the procedures o

250 gamma delta resolvases and the phage Mu Gin invertase, we used substrates that provided some but not

251 ranscripts for sucrose synthase and vacuolar invertase were both observed in the same cortical cells

252 The Km and Vmax values of immobilized invertase were found to be 39.4mmol/L and 349.5mmol/L mi

253 he structure and the catalytic properties of invertase were preserved, while Km values were slightly

254 dified secretory pathway cargos, Hsp150p and invertase, whereas stt4(ts) cells exhibit no detectable

255 ypeptidase Y, as well as in the secretion of invertase, which accumulates as a core-glycosylated form

256 This step was mediated by invertase, which had low activity.

257 the association of transcripts for vacuolar invertase with polyribosomes did not change over this pe

258 orresponded to genes encoding a soluble acid invertase with potential vacuolar targeting, which we ge