ライフサイエンスコーパス: nanodisc

1 lf-assembled native protein-lipid complexes (Nanodiscs).

2 he use of nanolipoprotein particles (NLPs or nanodiscs).

3 g interface of the P450-cytb5 complex in the nanodisc.

4 ane-bound protein YopB inserted into a lipid nanodisc.

5 narrow dispersity of lipid molecules in the nanodisc.

6 is via functional reconstitution of Aer into nanodiscs.

7 ture of the mouse TRPML1 channel embedded in nanodiscs.

8 r homodimers inserted into lipid bilayers of Nanodiscs.

9 n vitro self-assembling phospholipid bilayer nanodiscs.

10 d streamline analysis of heterogeneous-lipid Nanodiscs.

11 biotin transporter into phospholipid bilayer nanodiscs.

12 y in both protein assemblies and lipoprotein Nanodiscs.

13 al stability of the protein is higher in the nanodiscs.

14 platelets and assembled it into phospholipid nanodiscs.

15 lows the study of transmembrane signaling in nanodiscs.

16 example detailed herein, are included in the nanodiscs.

17 oluble nanoscale lipid bilayers, also termed nanodiscs.

18 ate the colocalization of the two enzymes in nanodiscs.

19 nally active membrane proteins inserted into nanodiscs.

20 ompared with nanorods and lower aspect-ratio nanodiscs.

21 PC1 that was incorporated into lipid bilayer nanodiscs.

22 ergy coupling of OpuA reconstituted in lipid nanodiscs.

23 gent-solubilized state or reconstituted into nanodiscs.

24 ve analyzed the translocation reaction using nanodiscs.

25 PG-nanodiscs, respectively, compared to POPC-nanodiscs.

26 protein complexes with GSL incorporated into nanodiscs.

27 o the elliptical shape recently reported for nanodiscs.

28 was monomeric in POPC-, POPC/POPG-, and POPG-nanodiscs.

29 ntermediate states for P-gp in lipid bilayer nanodiscs.

30 scherichia coli FF embedded in lipid bilayer nanodiscs.

31 with molecular dynamics studies of MSP1D1 in Nanodiscs.

32 were formed in the presence of bicelles and nanodiscs.

33 yloid polypeptide intermediate stabilized in nanodiscs.

34 nts after its cotranslational insertion into nanodiscs.

35 tificial lipid bilayers such as liposomes or nanodiscs.

36 and reconstitution of membrane proteins into nanodiscs.

37 constant amount of charge, for POPS and POPC Nanodiscs; 4) in contrast to some previous work, POPC on

38 e preparation of a range of stepwise-smaller nanodiscs (6- to 8-nm diameter) to overcome this limitat

39 The Nanodisc, a nanoscale membrane bilayer disc, is used to

40 -isolated lipids in purified KcsA-containing nanodiscs allows determination of preferential lipid-pro

41 ceptor linkage mechanism itself, building on nanodisc and electron cryotomography experiments.

42 This inaccessible fraction amounts to 66% in nanodiscs and 82% in micelles.

43 onstrate that a combination of lipid bilayer nanodiscs and a multiplexed silicon photonic analysis te

44 port cycle, we have reconstituted MalFGK2 in nanodiscs and analyzed its conformations under 10 differ

45 eceptors can be reconstituted as monomers in nanodiscs and as tetramers in liposomes.

46 econstituted into two lipid bilayer systems: nanodiscs and bicelles.

47 By combining nanodiscs and cell-free expression technologies, even co

48 constitution of the maltose transporter into nanodiscs and demonstrate that this system is ideally su

49 e combination of immobilizing the protein in Nanodiscs and footprinting with FPOP is a feasible appro

50 eractions between the transporter and MBP in nanodiscs and in detergent.

51 he transmembrane domains of mouse TMEM16A in nanodiscs and in lauryl maltose neopentyl glycol as dete

52 on by analyzing the conformation of MalFG in nanodiscs and in proteoliposomes.

53 analyses of synaptobrevin reconstituted into nanodiscs and into liposomes now show that most of its S

54 kDa transmembrane protein, was inserted into nanodiscs and labeled with deuterium oxide under native

55 nds and small diffusing particles, including nanodiscs and liposomes containing membrane protein rece

56 ed on 6 x 6 periodic arrays of both recessed nanodiscs and nanorings to elucidate the differences in

57 y, unlike nanospheres, larger-sized hydrogel nanodiscs and nanorods are internalized more efficiently

58 constructing membrane-like MCP2901-inserted Nanodiscs and phosphorelay activity assays, we demonstra

59 membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse

60 e complexity of the spectra in heterogeneous Nanodiscs and that the lipid composition can be determin

61 l-2-oleoyl-sn-glycero-3-phosphocholine lipid nanodiscs and the kinetics of activation at 15 degrees C

62 In the presence of nanodiscs and the two ligands, the equilibrium is signif

63 on of functional ion pumps incorporated into nanodiscs and their subsequent analysis by several bioph

64 des effective electron imaging of liposomes, nanodiscs and viruses as well as comprehensive visualiza

65 ed individually in nanoscale lipid patches, "nanodiscs", and directly immobilized on unmodified gold

66 nanoscale phospholipid particles (so-called nanodiscs) and measured their ability to bind arrestin.

67 nanometer scale discoidal membrane bilayers (nanodiscs), and potentials were measured using spectropo

68 sium channels, reconstituted them into lipid nanodiscs, and performed single-molecule FRET confocal m

69 rking with large membrane protein systems in nanodiscs, and provide guidelines on the setup of NMR no

70 led with LRET probes, reconstituted in lipid nanodiscs, and the distance between the NBDs was measure

71 In conclusion, nanodiscs are a powerful approach to study the important

72 the mechanism of formation of polymer-based nanodiscs are characterized by light scattering, NMR, FT

73 Arrays of nanodiscs are common in different types of applications,

74 Nanodiscs are compatible with various techniques, and du

75 Nanodiscs are examples of discoidal nanoscale lipid-prot

76 Nanodiscs are homogeneous, easily prepared with reproduc

77 Lipoprotein nanodiscs are ideally suited for native mass spectrometr

78 Nanodiscs are nanoscale lipid bilayers that offer a plat

79 Unlike detergent micelles, nanodiscs are native-like lipid bilayers that are well-d

80 Monodisperse lipid nanodiscs are particularly suitable for characterizing m

81 Lipid nanodiscs are playing increasingly important roles in st

82 These polymer-encased nanodiscs are promising platforms for studies of membran

83 Nanodiscs are protein-stabilized lipid assemblies that r

84 Nanodiscs are self-assembled approximately 50-nm(2) patc

85 Polymer-based nanodiscs are valuable tools in biomedical research that

86 the interface between a solution and a gold nanodisc array.

87 The substrates consist of sub-1-microm gold nanodisc arrays which display dimension-tunable plasmon

88 ngs were compared to the 6 x 6 recessed gold nanodiscs arrays.

89 cross-linking studies, the pump inserts into nanodiscs as a functional monomer.

90 , we not only demonstrated the usefulness of nanodiscs as a membrane-mimicking system, but also showe

91 Development of lipid nanodiscs as a membrane-protein-supporting platform, or

92 de a foundation for future studies utilizing nanodiscs as a platform for launching membrane proteins

93 highlight the potential of the use of native nanodiscs as a tool in the study of ion channels, and of

94 We used lipid bilayer nanodiscs as fusion partners; their rigid protein framew

95 resent work, we explore phospholipid bilayer nanodiscs as membrane mimics and employ electron microsc

96 probed the activities of these enzymes with nanodiscs as membrane mimics to determine whether they c

97 an does oligomeric rhodopsin in liposomes or nanodiscs, as assessed by stabilization of metarhodopsin

98 Furthermore, fluorescence anisotropy nanodisc assays revealed a direct physical interaction b

99 Here, we introduce nanodiscs assembled from shorter ApoA-I protein variants

100 Depending on the target protein of interest, nanodisc assembly and purification can be achieved withi

101 of full-length human TRPM4 embedded in lipid nanodiscs at 3-angstrom resolution, as determined by si

102 sented here are charge measurements on lipid Nanodiscs at 20 degrees C in 100 mM NaCl, 50 mM Tris, at

103 ding cassette exporter MsbA reconstituted in nanodiscs at 37 degrees C while it performs ATP hydrolys

104 characterizing electrostatic effects on the nanodisc attachment.

105 ly important property for the development of nanodisc-based characterization methodologies or biotech

106 These nanodisc-based investigations lay the prospects and guid

107 Here, we use a novel Nanodisc-based system, designed to be a soluble mimetic

108 how PI(3,4,5)P3, CaM, and membrane mimetics (nanodisc) bind to Akt(PHD).

109 phosphorylated light-activated Rh (P-Rh*) in nanodiscs binds arrestin-1 essentially as well as P-Rh*

110 V-ATPase conformations with the structure of nanodisc-bound Vo revealed that Vo is halted in rotation

111 st aspects of the insertion of proteins into nanodiscs by combining cell-free expression, noncovalent

112 dodecyl maltoside (DDM) and in phospholipid nanodiscs by monitoring the spatial positions of transme

113 bacteriorhodopsin, we show that our smaller nanodiscs can also be beneficial for the structural char

114 us of this protocol is on protein NMR, these nanodiscs can also be used for (cryo-) electron microsco

115 show here that small lipid bilayers known as nanodiscs can be used to chaperone the in vitro expressi

116 While nanodiscs can offer a near-native environment, the large

117 Nanodiscs carrying an inserted receptor dimer have high

118 Fractionated, clarified Nanodiscs carrying approximately five dimers per disc we

119 We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs

120 We describe here the analysis of nanodisc complexes by using native mass spectrometry (MS

121 oupled receptors in cotranslationally formed nanodisc complexes demonstrate the versatility of this a

122 hat is a hybrid between a NPL and an organic nanodisc composed of phospholipids and lipoproteins.

123 ontours of the light-adapted monomeric bR in nanodiscs composed of different lipid ratios exhibited h

124 tocycle intermediates of bR reconstituted in nanodiscs composed of different ratios of the zwitterion

125 h styrene:maleic acid moieties that can form nanodiscs containing a planar lipid bilayer which are us

126 n addition, charge measurements were made on Nanodiscs containing an Escherichia coli lipid extract.

127 We demonstrate the feasibility of adsorbing nanodiscs containing KR2 to an artificial bilayer.

128 spectra and lipid stoichiometries of intact Nanodiscs containing lipid-raft associated sphingomyelin

129 n of the P450-cytb5 complex in peptide-based nanodiscs, containing no detergents, has been demonstrat

130 rate that high-density lipoprotein-mimicking nanodiscs coupled with antigen (Ag) peptides and adjuvan

131 full-length, purified and reconstituted into nanodiscs, couples to G proteins upon direct activation

132 served between Akt(PHD) and PI(3,4,5)P3-free nanodiscs, demonstrating that PI(3,4,5)P3 is required fo

133 dies whereas the magnetic-alignment of macro-nanodiscs (diameter of > ca. 40 nm) can be exploited for

134 the effects of lipid headgroup chemistry on Nanodisc dissociation mechanisms.

135 Furthermore, we synthesized nanodiscs, each bearing a single lipid-embedded integrin

136 Strikingly, nanodiscs elicited up to 47-fold greater frequencies of

137 Nanodiscs eliminated established MC-38 and B16F10 tumour

138 allel screening of protein interactions with nanodisc-embedded lipids, glycolipids, and membrane prot

139 roscopy to elucidate the structures of lipid-nanodisc-embedded MsbA in three functional states.

140 s upon addition of the native ligand NADH to nanodisc-embedded VDAC-1 resemble those of micelle-embed

141 Using negative stain electron microscopy and nanodisc-embedding to provide a membrane-like environmen

142 termination, due to their smaller size these nanodiscs enable the investigation of interactions betwe

143 icon photonic microring resonator arrays and nanodiscs enables rapid interrogation of biomolecular bi

144 teraction between small-molecule ligands and nanodisc-encapsulated membrane proteins, because the res

145 kinetics of low molecular mass ligands with nanodisc-encapsulated membrane proteins.

146 een peptide and small-molecule ligands and a nanodisc-encapsulated potassium ion channel protein, Kcs

147 ulating the timing of the kinetics, with the nanodisc environment leading to an earlier Schiff base d

148 t neutral at pH 7.4; 2) high-anionic-content Nanodiscs exhibit polyelectrolyte behavior; 3) 3 mM Ca(2

149 nodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter

150 re-dependent structural changes, the polymer nanodiscs experience negligible structural evolution und

151 trates the utility of SSNMR experiments with Nanodiscs for examining lipid-protein interfaces and has

152 nts and pitfalls relating to optimization of nanodiscs for NMR spectroscopy and outline a strategy fo

153 Here we reconstituted Vo into lipid nanodiscs for single-particle EM.

154 es of this system and advantages provided by nanodiscs for structural and mechanistic studies of memb

155 e scaffold proteins (MSP) that do not affect nanodisc formation but shift the masses of nanodiscs in

156 n, has been incorporated in single copy into nanodiscs formed by a membrane scaffold protein encircli

157 Phospholipid nanodiscs (formed when a membrane scaffold protein encir

158 rative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates re

159 Nanodiscs generally exhibit more protection of membrane

160 e plasmon resonance (LSPR) arising from gold nanodisc gratings.

161 gly, we find that the monomeric rhodopsin in nanodiscs has a higher affinity for wild-type arrestin b

162 n this work, monomeric CYP3A4 solubilized in Nanodiscs has been studied for its ability to interact w

163 Insertion of the H(+)-ATPase into nanodiscs has the potential to enable structural and fun

164 The structure in nanodiscs has two Ca(2+) ions per monomer and its pore i

165 CYP3A4-Nanodiscs have absorption bands in the visible wavelengt

166 However, transport assays in nanodiscs have not been conducted so far, due to limitat

167 Phospholipid bilayer nanodiscs have recently been shown to be of great use fo

168 Endothelial cells also prefer nanodiscs, however those of intermediate aspect ratio.

169 In nanodiscs, however, an ensemble of pH-dependent conforma

170 Here, we report the discovery of polymer nanodiscs, i.e., discoidal amphiphilic block copolymer m

171 t nanodisc formation but shift the masses of nanodiscs in a controllable way, eliminating isobaric in

172 rotein can be reconstituted into the polymer nanodiscs in an active state.

173 AP complex was purified and reconstituted in nanodiscs in defined stoichiometry.

174 otein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spect

175 that membrane protein oligomers ejected from nanodiscs in the gas phase retain large numbers of lipid

176 nt micelles, bicelles, oriented bilayers, or nanodiscs, in order for them to be soluble or dispersed

177 otope effect on the steady-state turnover of Nanodisc-incorporated CYP17A1.

178 In summary, nanodisc-incorporated human SQR exhibits enhanced cataly

179 y showed that the negative surface charge of nanodiscs increased as the content of DOPG or DMPG was i

180 eptors, as higher loading of Aer dimers into nanodiscs increases kinase activity.

181 ow that Akt(PHD) binds to both layers of the nanodisc, indicating proper incorporation of PI(3,4,5)P3

182 isualized in membranes, vesicle-inserted and nanodisc-inserted, allowing us to reconstruct two virtua

183 A nanodisc is a lipid bilayer wrapped by membrane scaffold

184 Although it is commonly agreed that the nanodisc is plain disk shaped, several more advanced str

185 We found that although only one SNARE per nanodisc is required for maximum rates of bilayer fusion

186 id-bilayer presentation of viral antigens in Nanodiscs is a new platform for evaluating neutralizing

187 ic strength-gated ATPase activity of OpuA in nanodiscs is at least an order of magnitude higher than

188 show that the conformation of KcsA in native nanodiscs is very similar to that in detergent micelles,

189 e in fluorescence, initially quenched in the nanodisc, is monitored on a plate reader.

190 uction of intact purified alphaIIbbeta3 in a nanodisc lipid bilayer.

191 resonances of the heme chromophore in CYP3A4-Nanodiscs, LSPR spectroscopy is used to detect drug bind

192 usly and uniquely through application of the nanodisc membrane technology.

193 limitations of the two-dimensional nature of nanodisc membranes that offers no compartmentalization.

194 sm measurements performed on CYP3A4 bound to Nanodisc membranes were used to characterize the orienta

195 de a proof-of-concept demonstrating that the nanodisc model membrane system represents a promising ex

196 NMR methods to characterize Rheb tethered to nanodiscs, monodisperse protein-encapsulated lipid bilay

197 odopsin-octylglucoside micelle and the empty nanodisc (MSP1D1-Nd) using both MS and tandem-MS modes o

198 Nanodisc (ND) particles are soluble, stable, and reprodu

199 ZipA contained in nanodiscs (Nd-ZipA) retains the ability to interact with

200 We investigated this phenomenon in lipid nanodiscs (NDs) at equilibrium on a local scale, analyzi

201 with the CaR-ESI-MS assay implemented using nanodiscs (NDs) revealed that the PDs exhibited similar

202 y (ESI-MS) analysis combined with the use of nanodiscs (NDs) to solubilize glycolipids (GLs) has rece

203 d-release (CaR)-ESI-MS assay, which exploits nanodiscs (NDs) to solubilize glycolipids and mimic thei

204 aloganglioside ligand GM1, incorporated into nanodiscs (NDs), for cholera toxin B subunit homopentame

205 Here, we embedded human SQR in nanodiscs (ndSQR) and studied highly homogenous preparat

206 oscopy of membrane proteins in commonly used nanodiscs of 10-nm diameter were limited by the high mol

207 to solubilize membrane proteins and produce nanodiscs of controlled sizes.

208 Here we employed nanodiscs of defined phospholipid composition to quantif

209 servation of the tertiary structure of bR in nanodiscs of different lipid compositions.

210 A remarkable feature is that nanodiscs of different sizes, from nanometer to sub-micr

211 Our set of engineered nanodiscs of subsequently smaller diameters can be used

212 s of p75NTR, incorporated into lipid-protein nanodiscs of various sizes and compositions, by solution

213 t a protocol on the assembly of phospholipid nanodiscs of various sizes for structural studies of mem

214 fined lipid bilayer environment, lipoprotein Nanodiscs offer a promising cassette for membrane protei

215 Our results demonstrate that nanodiscs offer the potential for native mass spectromet

216 ly detergent micelles but also lipid bilayer nanodiscs or bicelles can serve as a means for the gentl

217 rified EGF-bound EGFR dimers in phospholipid nanodiscs or vesicles, suggesting that the environment a

218 primary preparations of receptor-containing Nanodiscs, otherwise heterogeneous for number and orient

219 d protein aggregates results in monodisperse nanodisc preparations ideal for structural and functiona

220 onditions, using self-assembled phospholipid nanodiscs prepared with the zwitter-ionic lipid 1-palmit

221 NMR) studies on lyophilized, rehydrated POPC Nanodiscs prepared with uniformly (13)C-, (15)N-labeled

222 However, native mass spectrometry of nanodiscs produces complex spectra that can be challengi

223 These results show that nanodiscs provide a much better membrane model than DPC

224 Nanodiscs provide a new and powerful tool for a broad sp

225 The cell-free synthesis in combination with nanodiscs provides a defined hydrophobic lipid environme

226 By employing differently sized nanodiscs reconstituted with single SecYEG complexes and

227 As with lipid nanodiscs, reconstitution of detergent-solubilized MsbA

228 membrane protein, essential for liposome or nanodiscs reconstitutions.

229 we infer that the ATPase activity of OpuA in nanodiscs reflects solute translocation.

230 2 orders of magnitude in POPC/POPG- and POPG-nanodiscs, respectively, compared to POPC-nanodiscs.

231 PECAM-1-containing nanodiscs retained not only their ability to bind homoph

232 detergent-free synthesis of membrane protein/nanodisc samples and the analysis by LILBID mass spectro

233 The solid-supported membrane assay with nanodisc samples provides reliable control over the ioni

234 Phospholipid nanodiscs seem promising to overcome the intrinsic probl

235 emonstrate that a well ordered dense film of nanodiscs serves for non-destructive, label-free studies

236 NMR data on nanodiscs show that a fraction of C2AB molecules bind to

237 f a domain 4-truncated PA pore inserted into nanodiscs showed that this domain does not significantly

238 For both proteins, the kinetics in nanodiscs shows the characteristics observed in the nati

239 detergent-solubilized MsbA into the polymer nanodiscs significantly enhances its activity.

240 we demonstrate that the combination of small nanodisc size, high deuteration levels of protein and li

241 hemoreceptor Tar from Escherichia coli using Nanodiscs, small ( approximately 10-nm) plugs of lipid b

242 We investigated this issue using Nanodiscs, soluble, nanoscale ( approximately 10 nm diam

243 To simplify interpretation of nanodisc spectra, we engineered a series of mutant membr

244 coupling measurements confirmed that in the nanodiscs, SRII photoactivation induces helix movement i

245 Furthermore, experiments using nanodiscs strongly suggest that autotransporter assembly

246 uting unlabeled LeuT in phospholipid bilayer nanodiscs, subjecting them to hydrogen-deuterium exchang

247 Exploiting the natural affinity of nanodisc-supported lipid bilayers for oxide-passivated s

248 g proper incorporation of PI(3,4,5)P3 on the nanodisc surface.

249 the SAXS data from the N-terminal truncated nanodisc system upon cholesterol incorporation.

250 lar to the particles formed in the so-called nanodisc system, which is based on N-terminal truncated

251 detailed comparative study of the ApoA1 and nanodisc systems upon cholesterol uptake.

252 This paper combines LSPR and Nanodisc techniques to optically sense drug binding to a

253 of fluorescence correlation spectroscopy and nanodisc technologies provides a powerful approach to ac

254 0 in the human heart, using a combination of Nanodisc technology and a nanohole plasmonic sensor call

255 Nanodisc technology provides membrane proteins with a na

256 ombining electron cryo-microscopy with lipid nanodisc technology to ascertain the structure of the ra

257 A pore and documented the value of combining nanodisc technology with electron microscopy to examine

258 Coupling nanodisc technology with HX MS offers an effective appro

259 omprehensive list of various applications of nanodisc technology with systematic analysis of the most

260 combination of cell-free synthetic biology, nanodisc-technology and non-covalent mass spectrometry p

261 Nanodiscs that hold a lipid bilayer surrounded by a boun

262 In contrast to lipid nanodiscs that undergo time- and temperature-dependent s

263 o change the membrane composition of the CPR-nanodisc, the redox potential of both flavins became mor

264 In nanodiscs, the ensemble of substates in the photoactivat

265 , binding of a PI(3,4,5)P3-embedded membrane nanodisc to Akt(PHD) with a 10(3)-fold tighter affinity

266 oli rendered water soluble by insertion into nanodiscs to (i) measure saturable binding of CheA and C

267 incorporated SRII-HtrII dimeric complexes in nanodiscs to allow unrestricted probe access to the cyto

268 say that uses suspended nanomembranes called nanodiscs to analyze fusion events.

269 ering pores connecting v-SNARE-reconstituted nanodiscs to cells ectopically expressing cognate, "flip

270 We addressed these questions using nanodiscs to create membrane environments in which recep

271 The ability of nanodiscs to trap amyloid intermediates as demonstrated

272 the native-like environment of phospholipid nanodiscs undergo spontaneous transitions between two di

273 The results indicate that P-gp in nanodiscs undergoes functionally relevant ligand-depende

274 The small-size nanodiscs (up to ca. 30 nm diameter) can be used for sol

275 icant amounts of lipid are released from the nanodiscs upon insertion of larger protein complexes.

276 onstration of a protein-protein complex in a nanodisc using NMR structural studies and should be usef

277 ured the affinity of arrestin-1 for P-Rh* in nanodiscs using a fluorescence-based assay and found tha

278 ctionally native receptor structure, we made Nanodiscs using natural and synthetic lipids, assaying e

279 ning of isotope-labeled membrane proteins in nanodiscs using nuclear Overhauser enhancement spectrosc

280 e discoidal proteolipid particles or "native nanodiscs." Using circular dichroism and tryptophan fluo

281 FERONIA protein kinase activity in nanodiscs was higher than that of soluble protein and co

282 cardiolipin, assembly of these proteins into nanodiscs was initiated.

283 Gq protein coupling to NTS1 in various lipid nanodiscs was significantly different, and the apparent

284 gle FoF1 molecules embedded in lipid bilayer nanodiscs, we now report the observation of Fo-dependent

285 asurements of F0F1 embedded in lipid bilayer nanodiscs, we observed that the ability of the F0 motor

286 hydrolysis, the NBDs of Pgp reconstituted in nanodiscs were never far apart during the hydrolysis cyc

287 ful preparation of novel peptide-based lipid nanodiscs, which are detergent-free and possesses size f

288 ical oxidation of proteins (FPOP), and lipid Nanodiscs, which are more similar to the native membrane

289 we provide evidence that reconstitution into nanodiscs, which are soluble disk-shaped phospholipid bi

290 o planar lipid bilayers directly from native nanodiscs, which enables functional characterization of

291 Chemoreceptors were inserted in Nanodiscs, which rendered them water soluble and allowed

292 ng resonator arrays and phospholipid bilayer nanodiscs, which together allow multiplexed screening of

293 brane that maintains the advantages of lipid nanodiscs while addressing their weaknesses.

294 n alphaIIbbeta3 embedded in a lipid bilayer (nanodiscs) while bound to domains of the cytosolic regul

295 same, the lateral pressure (profile) of the nanodiscs will differ from that of the vesicles.

296 Upon fusion of a nanodisc with a nonfluorescent liposome containing cogna

297 umber of lipids and eventual collapse of the nanodisc with release of the scaffold protein.

298 inally, we demonstrate the use of mixed belt nanodiscs with embedded membrane proteins to confirm the

299 Here we report the integration of nanodiscs with hydrogen exchange (HX) mass spectrometry

300 A4 incorporated in surface-immobilized lipid Nanodiscs, with and without the effector alpha-naphthofl