ライフサイエンスコーパス: ompA

1 The groEL-1, ompA, and omcB genes were used as markers for the early,

2 rological testing, outer membrane protein A (ompA) gene sequencing, and restriction fragment length p

3 he instability sequence also destabilized an ompA-bla fusion construct when fused to its 3'-UTR regio

4 reased half-lives of the rpsO, trxA, lpp and ompA transcripts.

5 dual full-length transcripts such as lpp and ompA varies significantly in wild-type cells.

6 pal-, lppA-, and ompA-deficient K. pneumoniae strains were constructed us

7 roles for Hfq: first, to bring both MicA and ompA into close proximity, and second, to restructure Mi

8 ipt (lpp) and two long transcripts (ompF and ompA).

9 ested PCR assays, targeting the 16S rRNA and ompA genes.

10 t are encoded by different tandemly arranged ompA genes.

11 train-typing using one or two genes, such as ompA, does not correlate with clinical phenotypes.

12 een a history of intragenic recombination at ompA including one instance of interspecies recombinatio

13 the major outer membrane protein, encoded by ompA, has many functional constraints and is under purif

14 rroborate complex developmentally controlled ompA expression in C. trachomatis.

15 oters of late-stage transcribed genes: ctrA, ompA (peptidoglycan-associated lipoprotein), bolA (stres

16 ent, and spacer length of the Flavobacterium ompA promoter by measuring the effects of site-directed

17 For ompA, all but five isolates had sequence identical to th

18 .15% for the housekeeping genes to 12.1% for ompA.

19 The strongest bias was for ompA in strain D (P = 3.3 x 10(-8)).

20 around the C. trachomatis genome apart from ompA.

21 recombination are identified downstream from ompA, which encodes the major outer membrane protein.

22 Analysis of the C. gallinacea ompA phylogeny revealed at least 13 well segregated vari

23 he major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omc

24 eeping genes and two membrane protein genes (ompA and espA) of 77 isolates that were diverse by PFGE

25 The promoters for two midcycle genes, ompA and pgk, were responsive to alterations in supercoi

26 GFP allows for the observation of changes in ompA expression in response to developmental signals.

27 based on nucleotide sequence differences in ompA, the gene that encodes the major outer membrane pro

28 We show that in many instances, ompA is a chimera that can be exchanged in part or as a

29 An isogenic ompA mutant showed reduced adherence compared to the par

30 patients overexpressed nonsignificantly more ompA than those from nonbacteremic patients in the unice

31 Isolates from PP overexpressed more ompA than those from colonized patients from the unicent

32 Additionally, we uncovered a new ompA promoter, which we named P3, utilizing the GFP repo

33 PCR amplification of ompA followed by Southern blot detection of amplicands r

34 cts could be corrected by complementation of ompA.

35 In addition, expression of ompA during wild-type UTI was sharply increased at time

36 3 from the multicenter cohort) Expression of ompA was determined with quantitative reverse-transcript

37 e of recombination in the downstream half of ompA.

38 Outside of ompA, trachoma strains differed primarily in a very smal

39 The region of ompA containing 13 tandem repeats was sequenced, reveali

40 , known to be involved in MicA regulation of ompA, may structurally remodel MicA to reveal the ompA-b

41 ly associated with some sequence variants of ompA and pmpEFGH.

42 genetic characterization of strains based on ompA, however, results in serovar groupings that are inc

43 s genetic diversity comes from serotyping or ompA genotyping, no quantitative assessment of genetic d

44 e encoding the major outer membrane protein (ompA) of Chlamydia trachomatis consists of a -35 hexamer

45 that encode immunodominant surface proteins (ompA and pmpEFGH) have been replaced by those characteri

46 icA is a small non-coding RNA that regulates ompA mRNA translation in Escherichia coli.

47 rollment and follow-up, 7 (20%) had the same ompA sequence at both visits, while 28 (80%) had discord

48 ate analysis in both cohorts together showed ompA overexpression as independent risk factor for pneum

49 mydia pneumoniae by a C. pneumoniae-specific ompA-based real-time PCR assay and 16S rRNA and 23S rRNA

50 signal sequences of beta-lactamase (bla SS), ompA, and phoA and the signal sequence and C-terminal pe

51 y PCR revealed that possession of two tandem ompA genes was widespread among this genus.

52 red to otherRickettsia-specific PCR targets (ompA,gltA, and the 17-kDa protein gene).

53 These data suggest that ompA overexpression is an associated factor for pneumoni

54 OmpA- E. coli with a plasmid containing the ompA gene restored the ability of OmpA- E. coli to inhib

55 1% of all case samples) was positive for the ompA gene.

56 which has a topology quite distinct from the ompA tree.

57 dependent self-association that occludes the ompA-recognition region.

58 can be accounted for by recombination of the ompA gene between different genomic backgrounds.

59 Disruption of the ompA gene in the tdcA mutant strain abolished the hypera

60 signed to target two variable domains of the ompA gene, VD2 and VD4.

61 into the 3' untranslated region (UTR) of the ompA gene.

62 e ppk gene showed increased stability of the ompA mRNA.

63 This defect in epithelial persistence of the ompA mutant was enhanced in competitive infections with

64 acer separating the -33 and -7 motifs of the ompA promoter also had a pronounced effect on promoter a

65 to restructure MicA to allow exposure of the ompA-binding site for pairing, thereby demonstrating the

66 insert had no effect on the half-life of the ompA-cat chimeric transcript.

67 may structurally remodel MicA to reveal the ompA-binding site for cognate pairing.

68 ains can be differentiated by sequencing the ompA gene encoding the outer membrane protein A (OmpA).

69 A primer set targeting the ompA gene (CP1-CP2/CPC-CPD) was used to perform a nested

70 hlamydiaceae: a multiplex test targeting the ompA gene and the rRNA intergenic spacer and a TaqMan te

71 By targeting the ompA gene, a real-time PCR assay has been developed to r

72 Microscopic examinations revealed that the ompA mutant formed significantly fewer IBCs, and those t

73 aumannii was decreased 2- to 3-fold when the ompA(Ab) gene was deleted.

74 c the conformational state of MicA where the ompA-binding site is exposed.

75 me extremely unstable in comparison with the ompA mRNA only when MazF(Sa) was induced in E. coli.

76 membrane protein (porB; 1,023 bp), with the ompA sequence (1,194 bp) used for reference.

77 ding amplification tests for C. trachomatis, ompA genotyping, and interviews and diary entries to doc

78 tain CtrA-binding motifs, and transactivated ompA, surE and bolA promoter-lacZ fusions in Escherichia

79 ng of the coding region of five transcripts, ompA, lpp, ompF, rpsA, and tufA.

80 The P. trehalosi ompA genes are highly diverged from those of M. haemolyt

81 emonstrate that whereas deletion of the UPEC ompA gene did not disrupt initial epithelial binding and

82 that predicting phylogenetic structure using ompA, which is traditionally used to classify Chlamydia,

83 izing element and was dominant to the 5'-UTR ompA and REP stability elements.

84 Transcriptional fusions with ompA and bla have been used to identify a novel mRNA ins