ライフサイエンスコーパス: second dimension

1 00 (110 in the first dimension and 38 in the second dimension).

2 pillaries that provide the separation in the second dimension.

3 mension and capillary electrophoresis as the second dimension.

4 nd loss of resolution and sensitivity in the second dimension.

5 e first dimension; only six co-eluted in the second dimension.

6 rform sensitive detection of weak acids in a second dimension.

7 ses (at high flow rates) are required in the second dimension.

8 lumn is analyzed at least three times in the second dimension.

9 ns during the limited separation time in the second dimension.

10 rap columns and subsequently analyzed in the second dimension.

11 um NSP-35 nanostationary phase column in the second dimension.

12 reversed phase separation in the orthogonal second dimension.

13 dentified and quantified after separation in second dimension.

14 ography at critical conditions (LCCC) in the second dimension.

15 1)D) column for further resolution on a long second dimension ((2)D(L)) column for parallel detection

16 ns were then modulated and separated using a second dimension ((2)D) column via GC x GC, resolving th

17 until their sequential reinjection onto the second dimension ((2)D) column.

18 ployed multiple channels (4 channels) in the second dimension ((2)D) to increase the (2)D separation

19 such methods are applied orthogonally to the second dimension ((2)D), background correction is dramat

20 simple alkane mixture using the RTIL-coated second-dimension ((2)D) mucolumn produced reasonably goo

21 y one or more detectors at the outlet of the second dimension, (2)D, with very short runs to avoid un

22 In the second dimension, 30 s gradients at a cycle time of 1 mi

23 The second dimension accounted for phylogenetic change towar

24 The use of the second dimension allowed for successful fragment assignm

25 ization mass spectrometry (MALDI-MS), as the second dimension, allows precise determination of the ma

26 Second-dimension analyses are performed and completed ev

27 ray of parallel channels were tested for the second dimension, and improved performance was observed

28 using ultrafast chiral chromatography in the second dimension are successfully applied to the separat

29 en the solvent systems used in the first and second dimensions as a major obstacle.

30 th conventionally sized columns in the rapid second dimension, as solvent consumption is drastically

31 ontaining fractions are then analyzed in the second dimension by either MALDI-PSD or nano-ES with pre

32 ons are transferred across an interface to 5 second-dimension capillaries, and analyte is detected by

33 cally transferred across an interface into a second dimension capillary, where components are further

34 The second-dimension capillary contains an SDS buffer for mi

35 ides were rapidly sampled into a 1.3-cm-long second-dimension channel, where they were separated by c

36 orthogonally intersected a high-aspect ratio second-dimension channel.

37 In a second dimension chromatography seleno-amino acids were

38 oose virtually any combination of first- and second-dimension column diameters without loss in system

39 lumn that is typically narrow and long and a second-dimension column that is wide and short.

40 illary with a PDMS stationary phase, and the second-dimension column was a 0.5 m long, 100 mum i.d. c

41 The effluent exiting the second-dimension column was in the range 6-8 mL/min, wit

42 in eluent flow rate and column length of the second-dimension column, and (5) the maximum achievable

43 y is coupled to a 0.075 mm internal diameter second-dimension column.

44 at is, with three to four separations on the second dimension (column 2) per peak width from the firs

45 capacities can be achieved by using shorter second-dimension columns and collecting a relatively lar

46 l liquid chromatography that makes the rapid second dimension compatible with mass spectrometry witho

47 The second dimension consisted of a standard reversed-phase

48 iltration column was then transferred to the second dimension consisting of a monolithic C-18 column

49 The second dimension consists of a 1.2 cm x 1.2 cm mucolumn

50 The second dimension consists of discontinuous SDS-PAGE.

51 ted fractions were then characterized by the second dimension (D2) size exclusion chromatography (SEC

52 on to a range suitable for microinjection by second dimension electrophoresis and enzymatic digestion

53 onged time periods necessary to complete the second-dimension electrophoretic separation step--denatu

54 velocity and initial organic content of the second-dimension eluent.

55 -CGE) were repetitively transferred into the second dimension every 0.5 s of run time in the first di

56 dimension was repetitively injected into the second dimension every few seconds.

57 trated abundant fragment ions and provided a second-dimension "fingerprint" of the complex cellular f

58 use of ultrafast chiral chromatography as a second dimension for 2D chromatographic separations.

59 e ( approximately nanosecond) signals adds a second dimension for multiplexing and also permits detec

60 ichment, reversed phase nanoLC column in the second dimension for separation, a benchtop Orbitrap mas

61 pidly sampling and analyzing effluent in the second dimension from the first dimension.

62 In the second dimension, functionalized monolithic columns are

63 sequence, due to the time restriction of the second-dimension gradient time, online 2D-LC schemes can

64 y using nonporous reversed-phase HPLC in the second dimension (IEF-NP RP HPLC).

65 se of chiral stationary phases (CSPs) as the second dimension in 2D-LC, especially in the comprehensi

66 design of gradient elution strategies in the second dimension in combination with photodiode array de

67 ins, linking the analysis with a MALDI-based second dimension in m/z is shown to be an efficient meth

68 c of the protein while migration time in the second dimension is characteristic of the peptide.

69 composition of the mobile phase used in the second dimension, its initial organic content if this se

70 we show how extending pi-conjugation in the second dimension leads to novel materials with HOMO-LUMO

71 the relatively high flow rates used for the second dimension make direct (splitless) hyphenation to

72 (nLC) separation is coupled directly with a second dimension micro free flow electrophoresis (muFFE)

73 ing as a limitation when incorporated as the second dimension of a 2D separation.

74 F or NEPHGE tube gel before using it for the second dimension of a two-dimensional gel.

75 The second dimension of difficulty concerns the intermodel d

76 Temperature programming in the second dimension of GC x GC was able to improve separati

77 then released after a delay to initiate the second dimension of IM.

78 111 capillary column is enhanced by adding a second dimension of separation ((2)D) in a GC x GC desig

79 electrophoresis is implemented to provide a second dimension of separation.

80 ed as the separation modes for the first and second dimension of the electrophoresis, respectively.

81 SEC at ultrahigh-pressure conditions in the second dimension offered very fast, yet efficient separa

82 id points simultaneously along the first and second dimensions on the basis of applying a one-dimensi

83 ed according to the product of the first and second dimension peak capacities.

84 k widths, leading to significantly increased second dimension peak capacity.

85 city is simply the product of the first- and second-dimension peak capacities.

86 observed when the ratio of the first- to the second-dimension peak capacity is much less than unity.

87 parations made with online 2D-LC require the second-dimension peaks to be very narrow, (2) the separa

88 tive mapping will extend that inference to a second dimension representing index species of the 20 li

89 e/interference peak height ratio, first- and second-dimension resolutions, signal-to noise ratio, inj

90 This gives access to calculated second dimension retention indices ((2)I).

91 impact of chromatographic variability on the second dimension retention time, a concept based upon hy

92 information about certain parameters (e.g., second-dimension retention time variability, first-dimen

93 l complications are observed when first- and second-dimension retention times show some correlation,

94 eparation of individual molecular species by second-dimension reverse-phase HPLC and characterization

95 of these peptides in the flow-through by the second-dimension RP trap can dramatically reduce the com

96 roduction by allowing the use of the longest second dimension run time, while maintaining quantitativ

97 If second dimension SDS-PAGE followed BNP, the 180-kDa muta

98 y of P13 within the complex was confirmed by second dimension SDS-PAGE, Western blotting, mass spectr

99 separation, relative retention time for the second dimension separation (2DrelRT) and boiling point

100 us sub-3-mum stationary phase provide a fast second dimension separation and a sufficient sampling fr

101 separations are limited by the speed of the second dimension separation and the consequent loss of p

102 k capacity lost due to under sampling by the second dimension separation as peaks elute off the first

103 at the base, (1)w(b) of ~3 s and varying the second dimension separation run time from 300 to 2900 ms

104 ations are used, instead of simply using the second-dimension separation as a desalting step.

105 mpled in parallel by 20 channels effecting a second-dimension separation by native electrophoresis.

106 comprising silicon-micromachined first- and second-dimension separation columns and a silicon-microm

107 o-run variation by including 7 M urea in the second-dimension separation matrix.

108 o sample the (1)D with an adequate number of second dimension separations.

109 Over 100 transfers and second-dimension separations are performed over an appro

110 apacity and resolution when high-performance second-dimension separations are used, instead of simply

111 High field strength (+2 kV/cm) enables rapid second-dimension separations in which each peak eluted f

112 lex might be specific for leaf growth in the second dimension, since it is not present in Poaceae (gr

113 sence of sodium dodecyl sulfate (SDS) in the second-dimension sizing separation limits the orthogonal

114 ectrophoresis can be conveniently coupled to second-dimension sodium dodecyl sulfate-polyacrylamide g

115 ved in gel shift assays has been resolved by second-dimension sodium dodecyl sulfate-polyacrylamide g

116 Commonly applied SEC separations in the second dimension take several minutes, so that a total L

117 ks are compounds with retention times in the second dimension that are longer than the modulation per

118 hy separating according to molar mass in the second dimension, thus providing comprehensive informati

119 The addition of a second dimension to detection probes permits the use of

120 n and an ultrahigh-pressure LC system in the second dimension to ensure maximum sensitivity and perfo

121 quid chromatography (RP-UHPLC) column in the second dimension to further separate the ester species.

122 e size-exclusion chromatography (SEC) in the second dimension to separate the constituting polymer mo

123 As the second dimension to the genome, the epigenome contains k

124 t restriction endonucleases in the first and second dimensions, to generate filters suitable for imag

125 ous and 2.7 mum fused-core particles) in the second dimension, together with the use of 0.1% phosphor

126 Discrete peaks in the second dimension using the thermal modulator were 30-55%

127 Next, protein transfer to the second dimension was accomplished by chemical mobilizati

128 This second dimension was capable of analyzing all fluid volu

129 imension and the same RP-UHPLC method in the second dimension was developed to analyze the degradatio

130 etween angiosperms and gymnosperms), and the second dimension was related to changes in maximum tree

131 To overcome this problem, the second dimension was temperature-programmed by resistive

132 could be introduced into both the first and second dimensions was also illustrated.