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1 set and by learning several Atari 2600 games sequentially.
2 ectors or two measurements must be performed sequentially.
3 h natural and manmade, are often constructed sequentially.
4 y even be combined with RT, concurrently and sequentially.
5 /s targeting Abeta and tau simultaneously or sequentially.
6  and GlcNAc residues to the protein backbone sequentially.
7  and GlcNAc residues to the protein backbone sequentially.
8 toichiometries of target complexes that work sequentially.
9 tiple components that work cooperatively and sequentially.
10 cesses controlled by caudal M1 in large part sequentially.
11 ng photons through a specimen multiple times sequentially.
12 measure the range of each pixel in the scene sequentially.
13 examined by high definition endoscopy and SE sequentially.
14 hus substrate preference, simultaneously and sequentially.
15 key secondary safety endpoints were assessed sequentially.
16 ical or amplification-based assays performed sequentially.
17         Murine forepaw ossification occurred sequentially.
18 g., spinal cord) are thought to be assembled sequentially [10-14], making this an ideal brain region
19 xocytotic responses are believed to mobilize sequentially a readily releasable pool of vesicles (RRP)
20 cumulates primarily in vacuoles and does not sequentially accumulate in the cytosol.
21 , and a flow pattern whereby quality changed sequentially across days (stroke-unit admission within 4
22 ade is lifted and charge carriers can tunnel sequentially across the junction.
23 ent proteins can easily be extended to other sequentially acting enzymes and lays down principles for
24 ogramming process, the fiber families can be sequentially activated to bend when the temperature is i
25 s using two different electrophiles that are sequentially activated with exquisite selectivity under
26 ed tetramanganese calcium (Mn4O5Ca) complex, sequentially activates two substrate water molecules gen
27 ClBcat reagent serves a bifunctional role by sequentially activating the alkyne, despite being less e
28 tein N- and O-glycosyltransferases (PGTases) sequentially add monosaccharides onto the target protein
29 prazosin 5 mg/d, and rilmenidine 1 mg/d were sequentially added at monthly visits if home blood press
30                            Lettuce juice was sequentially added into FC solution with FC periodically
31                   Calcium and phosphate were sequentially added to protein solutions without and with
32 ssembly, trimeric capsid building blocks are sequentially added to the growing capsid, with pentamers
33  proteins and additional glutamates are then sequentially added via alpha-carboxyl-linkages to the gr
34  Cox proportional hazards modeling to assess sequentially adding baseline PCWP and change in CI to 5
35               Discrete acoustic sources were sequentially addressed by scanning a focussed optical be
36  crush paradigm, mitochondrial clusters form sequentially adjacent to the lesion site and oxidation s
37 ian, Mexican, Puerto Rican and Cuban), after sequentially adjusting for maternal, socio-economic and
38 emerging from such studies is the ability of sequentially administered, modified immunogens (designed
39 9 [1.11-1.50]) in women who used AIs only or sequentially after tamoxifen (1.26 [1.09-1.45]) vs tamox
40 n; this activity-dependent maturation occurs sequentially along the classic 'tri-synaptic' pathway, f
41  that the CLIs directly activate motoneurons sequentially along the segments during larval locomotion
42 ein, Titin and Haptoglobin, were found to be sequentially altered with respect to increased parasite
43 either evofosfamide or erlotinib alone, (ii) sequentially alternating single doses of each drug leads
44 teristics, and virologic and safety outcomes sequentially and assessed quality independently.
45 eage specific subnetworks that are activated sequentially and control the differentiation process in
46 ltiple bonds along a single HA chain rupture sequentially and independently under load.
47 w that SC plasticity becomes restricted in a sequentially and spatially choreographed program, culmin
48  of drift-diffusion models that are arranged sequentially and that are resetting in each SCT tap.
49       Subjects made two perceptual decisions sequentially and were rewarded only if they made both co
50  of cellular metabolism is often carried out sequentially and with low throughput.
51  signals and transcriptional inputs that act sequentially and/or in parallel.
52  nanoparticles (administered as a mixture or sequentially) and to measure the distribution and correl
53 of diffusion along threading dislocations in sequentially annealed nitride metal/semiconductor superl
54 or transdermal drug and vaccine delivery, by sequentially applying the ultrasound then the therapeuti
55 galovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly charac
56 MS23 along with MS32, bHLH122 and bHLH51 act sequentially as either homo- or heterodimers to choreogr
57 ss simultaneously, most insects add segments sequentially as the germband elongates.
58  charge transfer and dissolution steps occur sequentially as the synchronicity between the EC and opt
59 bind retromer through the VPS26 protomer and sequentially assemble a ternary complex with PTHR and SN
60 aphylococcus epidermidis (groups I-III) were sequentially assessed against a newly developed Staphylo
61 dosing schedules, to which participants were sequentially assigned as follows: once daily, intermitte
62 Participants from the NIH were non-randomly, sequentially assigned in cohorts of three to receive mon
63 5, we enrolled 44 patients, of whom ten were sequentially assigned to CUDC-907 once-daily (MTD 60 mg)
64                                Patients were sequentially assigned to lenalidomide 25 mg/day, days 1-
65                14 patients were enrolled and sequentially assigned to receive intrathecal HPbetaCD at
66 h during which monomeric alpha-syn molecules sequentially associate with existing seeds.
67 lorimetry that the ETS member PU.1 dimerizes sequentially at a single sequence-specific DNA-binding s
68 rd 3 + 3 cohort design and assigned patients sequentially at trial entry according to the 3 + 3 dose-
69 ha-Gal and beta-Man terminating antennae can sequentially be decaged by an appropriate glycosidase to
70 iazanes, a class of cyclic organic compounds sequentially bearing three all-saturated nitrogen atoms
71 ensor/counter/reference electrode assemblies sequentially between the samples.
72  cells treated with autophagic inhibitors to sequentially block the autophagic flux suggest that FoxO
73 roach consists of a pipeline that integrates sequentially building ssDNA secondary structure from seq
74 Enhanced electrode conductivity was achieved sequentially by constructing a conductive polymer (PEDOT
75           Protein ubiquitination is mediated sequentially by ubiquitin activating enzyme E1, ubiquiti
76 ion (HR) and replication fork protection are sequentially bypassed during the acquisition of PARPi re
77 Th17 (LTK63)-prone immune responses and were sequentially challenged with mouse-adapted influenza vir
78 eptor (PAM), we show that both FI-FH and hPm sequentially cleave C3b.
79  import into the matrix compartment, Prx1 is sequentially cleaved by mitochondrial processing peptida
80                          Eight contacts were sequentially cohoused with each of the donors for 24 hou
81                                              Sequentially cohousing AMER-colonized mice with two diff
82 e cation and anion exchange reactions can be sequentially combined while preserving the original NC s
83 e-to-face CBT sessions of 50 minutes each or sequentially completed 11 internet modules and had weekl
84 f the dual-function rotaxane catalyst can be sequentially concealed or revealed, enabling catalysis o
85 s, first and second heart fields (FHF, SHF), sequentially contribute to longitudinal subdivisions of
86                        The cyclobutenone was sequentially converted into a highly substituted carbazo
87 activations and FA assembly are strongly and sequentially correlated, with the concurrent FA assembly
88 shift on the type of collagen expression was sequentially delayed (around 21 days).
89 rontal cortex and nucleus accumbens, are not sequentially dependent during contextual decision-making
90 ependencies: the appearance of scene gist is sequentially dependent on the gist perceived in previous
91 se spray-coating under ambient conditions to sequentially deposit compact-TiO2, mesoporous-TiO2, CH3N
92 t the order in which distinct RNA inputs are sequentially detected.
93 hat the designed immunosensor can be used to sequentially detection of CA 125 and CA 15-3 markers wit
94         Cohorts, including approximately 300 sequentially diagnosed RR-TB patients per South African
95      The conditions were optimized to couple sequentially different moieties in one pot.
96 rd and effective approach based on stepwise, sequentially directed disulfide bond formation, exemplif
97 erimental conditions in two structurally and sequentially distinct protein scaffolds.
98  molecular clock estimates, and Pentapetalae sequentially diverged into eight major lineages within c
99 tified and shown to specifically bind to two sequentially divergent IQ motifs of the Dictyostelium my
100 ting striking structural homology with other sequentially diverse KA1 domains.
101 tion, enhanced endocytosis and intracellular sequentially drug release.
102 cify two distinct regulatory entities acting sequentially during B-cell ontogeny.
103 at enrollment (baseline) in all patients and sequentially during hospitalization in 255.
104 us and antibody titres measurements recorded sequentially during infection from 53 Vietnamese dengue
105 to patients would be to combine these agents sequentially, enabling the use of existing phase I clini
106  a droplet generating microfluidic device to sequentially encapsulate the eluting compounds.
107 s oculomotor-induced temporal modulations to sequentially encode different spatial components and sug
108 larity protein Partner of Inscuteable (Pins) sequentially enriches Rok and Protein Kinase N (Pkn) on
109                    Twenty healthy adults, in sequentially enrolled groups of 10 each, received vaccin
110 age I, 148 volunteers aged 1.5-45 years were sequentially enrolled into 4 age-descending groups and r
111                             In this phase 2, sequentially enrolled, multicohort, multicentre, non-ran
112 ) with no history of rabies vaccination were sequentially enrolled.
113 atterns in a separate cohort of 25 patients, sequentially examined with both tracers.
114  two further SuFExable handles, which can be sequentially exchanged to create 3-dimensional covalent
115               The different cavity modes are sequentially excited by shifting the feedback mirror ang
116 st, where the requisite activation modes are sequentially exploited as the reaction proceeds, is an a
117                                 The platform sequentially exploits the enrichment and depletion pheno
118 development, neural stem cells (neuroblasts) sequentially express transcription factors that generate
119 analysis revealed five unique miRNA patterns sequentially expressed at five sampled time points, like
120 ed soil solution P, microbial biomass P, and sequentially extracted inorganic and organic P pools fro
121            We characterized EXPB1 binding to sequentially extracted maize CWs, finding that the prote
122  exploit the full value of plant material by sequentially extracting and valorising its components.
123 variably with group A molecules and expanded sequentially first to group B and finally to group C mol
124 er subdivided into three sub-classes engaged sequentially, first at slow then intermediate and finall
125 ion occurs only after S proteins are cleaved sequentially, first during their secretion through the e
126 imulation, GIV modulates Galphai and Galphas sequentially: first, a key phosphomodification favors th
127                           The helical bundle sequentially folded in an N-terminal domain (NTD) and a
128                         Stove were installed sequentially for 2 weeks and kitchen air monitoring was
129 s prepared in-situ by UV crosslinking and by sequentially freezing followed by freeze drying of the c
130 ombination were assessed in strains obtained sequentially from a patient after 7.4 years of infection
131 erm arthropods, posterior segments are added sequentially from a segment addition zone (SAZ) during e
132 inical isolates of Escherichia coli obtained sequentially from a single patient during a course of an
133 gorithm 3progresses through each time series sequentially from beginning to end, thus enabling real-t
134 urons were segmentally arrayed and activated sequentially from the posterior to anterior segments dur
135 nwheel structure around which activity flows sequentially from visual to barrel then to hindlimb soma
136 ociated with current and future targets when sequentially harvesting targets.
137                                           In sequentially hermaphroditic (sex-changing) fish, the sex
138                                              Sequentially hermaphroditic fish change sex from male to
139 rogesterone [45 mg] vaginal gel administered sequentially [i.e., once daily for 10 days of each 30-da
140 nts, can accelerate the discovery process by sequentially identifying the next experiments or calcula
141 yers within a normal TIRF excitation zone by sequentially imaging and photobleaching the fluorescent
142 cies developed memoranda of understanding to sequentially implement 3 protocols addressing key Nation
143 orating point-of-care hemostatic testing was sequentially implemented at 2 hospitals at a time in 1-m
144 neural development, sensory pathways develop sequentially in a feedforward manner, whereby each local
145 yptococcal infection and disease development sequentially in a single host.
146 identified SoxC and Brn1/2/4, which function sequentially in the neurogenic regulatory pathway and ar
147 ic mutations are mutually exclusive or occur sequentially in the same subclone of cells.
148 und that this early activation step occurred sequentially in the three protomers, generating two asym
149        fMRI changes during seizures occurred sequentially in three functional brain networks.
150 n 90min after liposome infusion administered sequentially in three regions of the tumor.
151 e developing embryo, Hox genes are activated sequentially in time and space according to their genomi
152  genetic variants and obesity were validated sequentially in two case-control cohorts.
153 n that events in biomolecular networks occur sequentially, in a cascade-like manner, and ask whether
154             The two guests could be released sequentially, in a specified order defined by the input
155 used pattern of expression requires auxin to sequentially induce auxin efflux and influx carriers PIN
156 n modes of (-)-riboflavin, it is possible to sequentially induce isomerization and cyclization by ene
157 adth and magnitude of antibody responses, we sequentially infected mice, guinea pigs and ferrets with
158 ents, with associated mitigation plans, were sequentially initiated and have increasingly influenced
159                  We show here that the EB is sequentially innervated by small-field and large-field n
160 orm sensory input into action, these regions sequentially integrate eye- and direction-specific senso
161 l and analog circuits are implemented on two sequentially integrated layers of devices.
162 -specific co-regulators Kaiso and Sox10 that sequentially interact with Tcf7l2 to coordinate the swit
163 The vertebrate complement system consists of sequentially interacting proteins that provide for a rap
164 nized mice, which were able to differentiate sequentially into intermediate and nonclassical cells.
165                   Participants were enrolled sequentially into one of three dose cohorts (low dose [o
166                                           We sequentially isolated H. pylori (strain J99) from a pati
167 des by spin-coating different-sized QDs in a sequentially layered manner, thereby creating an energet
168 LlaBIII were structurally well-conserved but sequentially less-conserved.
169                                       AF was sequentially mapped in both atria with a 20-pole PentaRa
170                      We applied the pairwise sequentially Markovian coalescent approach on the genome
171  1.36), and 1.25 (95% CI, 1.19 to 1.31) when sequentially matched for comorbidity, insurance, tumor c
172 serum glucose and electrolytes, and P/F were sequentially measured, and medians (interquartile ranges
173 l gyrus volumes and poor emotional awareness sequentially mediated the association between early ACEs
174                        This compound is then sequentially modified by enzymes in the E. coli serotype
175                      Pyrazole cores are then sequentially modified through additional reactor modules
176               When the methylation states of sequentially neighboring regions are unknown, the accura
177              Using the methylation states of sequentially neighboring regions as one of the learning
178 posited within different organs, formed from sequentially non-homologous polypeptide chains and affec
179 ntire Protein Data Bank and identify several sequentially nonhomologous chains that form a Hopf link
180 nvestigator, with the use of opaque, sealed, sequentially numbered envelopes that each contained one
181 ndomly assigned (1:1) in blocks of two using sequentially numbered sealed envelopes to glycaemic regu
182     Randomization codes were kept in sealed, sequentially numbered, opaque envelopes.
183 nd eight) with concealment by use of opaque, sequentially numbered, sealed envelopes, to receive labo
184 rption which can be represented by a pair of sequentially-occurring pseudo-first order reactions.
185                    Although sentences unfold sequentially, one word at a time, most linguistic theori
186 m size that can be solved with conventional, sequentially operating electronic computers.
187  and hydrophobicity of the sponge shunt were sequentially optimized, and achieved 10-fold signal enha
188 ays (e.g., whether the signals are processed sequentially or concomitantly when two pathways are requ
189 tion of postoperative chemoradiation (either sequentially or concomitantly) after esophagectomy was a
190 f chemotherapy to radiation therapy, whether sequentially or concurrently, was a strong prognostic fa
191  suggest that the VgrG1 C terminus interacts sequentially or simultaneously with the Tap-1-Tde1 compl
192 nv) are activated in relation to each other, sequentially or simultaneously.
193 reaction steps are performed simultaneously, sequentially, or in flow.
194 ne expression is co-ordinately regulated and sequentially ordered during productive infection.
195 n (~20min each) HIFU treatments administered sequentially over an hour in a large volume of mouse xen
196 n ( 20min each) HIFU treatments administered sequentially over an hour in a large volume of mouse xen
197                        Two H5N8 viruses were sequentially passaged in BALB/c mice and plaque-purified
198 benefit (i.e., lower mean squared error) for sequentially passing a propensity score through each dat
199 oped by taking advantage of the provisional, sequentially Pd-catalyzed one-pot generation of alkynone
200                         CCs autonomously and sequentially performed all liquid delivery steps require
201 and Notch) that pattern the neural tube were sequentially perturbed to identify an optimized combinat
202  Mps1 promotes checkpoint activation through sequentially phosphorylating Knl1, Bub1, and Mad1.
203                 Visual short-term memory for sequentially presented coloured bars of different orient
204 ed to encourage participants to maintain the sequentially presented items together in WM.
205 s and relative/ordinal judgments between two sequentially presented orientations.
206 the judgment of emotional congruence between sequentially presented pairs of stimuli belonging to the
207 that required them to remember three or four sequentially presented pictures across a brief delay.
208                 In this task, observers were sequentially presented with two shape stimuli and a retr
209 ce, tumor characteristics, and treatment) in sequentially, propensity-scored, optimally matched patie
210 ke (DBL) domains from different alleles were sequentially purified from plasma pooled from multigravi
211                       Patients (n = 81) were sequentially randomized 1:1 to receive TTFC or TRAV+TIM
212 voring the view that these NHEJ proteins are sequentially rather than concurrently recruited to DNA e
213 gorithm applying four lung function measures sequentially (ratio of forced expiratory volume in 1 s [
214 d cascade MDFF and resolution exchange MDFF, sequentially re-refine a search model against a series o
215                         The mine wastes were sequentially reacted in flow-through columns at pH 7.9 (
216        We find that two motor pools that are sequentially recruited during locomotion have identical
217 pation of multiple types of cells, which are sequentially recruited during the healing process.
218                                 Total of 112 sequentially recruited patients.
219 nning form into an effector mRNP particle by sequentially recruiting the CCR4-NOT complex, decapping
220 s from the carbon and hydrogen of CH4, which sequentially reduce SnO2.
221 scaffolds with distinct fiber fractions that sequentially release active collagenase (to increase ECM
222 f6 and Loc1 facilitate Rpl43 loading and are sequentially released from 60S after incorporation of Rp
223 zymes were metamorphosed into one another by sequentially replacing the loops and helices of the six-
224 s (RBPs)-PUF-8, MEX-3, GLD-1, and CGH-1-that sequentially repress the CED-3 caspase in distinct regio
225                     This template contains a sequentially reversed "Arg-(pI)DPhe" motif with respect
226 ians, two radiologists, and two pathologists sequentially reviewed clinical-radiologic findings and b
227  al. provides novel evidence that our brains sequentially sample different objects in a visual scene.
228        Crucially, the different objects were sequentially sampled at a rate of about 2 Hz.
229                                           We sequentially sampled cohorts of coral reef fishes in the
230           Second, the same naive library was sequentially screened for phage exhibiting TM binding, i
231                                              Sequentially screened patients with sleep apnea suspicio
232 ock-like regulation of PR stripe addition in sequentially segmenting species while highlighting regul
233 ng, and sensorimotor contingency that do not sequentially separate sensory and motor brain processes.
234 (fm) changed randomly (RAN) across trials or sequentially (SEQ) after several repeated trials.
235  followed by enhancer-activation by CBP/p300 sequentially shape dynamic enhancer landscapes during ce
236  Diffusion-encoding directions were acquired sequentially, spatiotemporally registered, and retrospec
237                 Screening was then optimized sequentially, starting with the youngest age group, and
238 l poliovirus vaccine (OPV) are being removed sequentially, starting with type 2 in April 2016.
239                     Two IL17 subfamilies are sequentially strongly upregulated and attenuated in the
240 ssion panel and stem cell panel were used to sequentially subtype individual fluorescently stained pa
241 ed that specific functions tend to be gained sequentially, suggesting that evolution in prokaryotes i
242  emerging tumor niche, we observed that MSCs sequentially surrounded the BCCs, promoted formation of
243  goal a time switching strategy known as the Sequentially-Switched Delay Line (SSDL) is proposed.
244  morphology is induced by D-enantiomers, and sequentially switching between amino-acid enantiomers ca
245 ynthesize a holographic color image, one can sequentially take three holograms at different wavelengt
246 ane-coated biomimetic nanocarrier, which can sequentially target the bone microenvironment and myelom
247 proteins that were highly phosphorylated are sequentially targeted by the phosphatase PP2A-B55, but w
248           This is an example of a miRNA that sequentially targets both negative and positive regulato
249 bias voltage varies, the photodiodes exhibit sequentially the behavior of three different colors, cor
250 he hydrosilylation of CO2 by R3SiH to afford sequentially the bis(silyl)acetal, H2C(OSiR3)2, and CH4
251                                              Sequentially, the quaternary ammonium salts were treated
252 g pixel super-resolution and phase retrieval sequentially, they require large amounts of data, which
253 laced on models in which stem cells progress sequentially through a one-way proliferative hierarchy.
254 and analyzed individual CD8(+) T lymphocytes sequentially throughout the course of a viral infection
255 Thus, neuropeptide modulation is required to sequentially time outputs from a network of synchronous
256 he activity of transcription factors binding sequentially to a series of transient early and late enh
257 lear receptors recruit multiple coactivators sequentially to activate transcription.
258 tein factors bind stably to the promoter DNA sequentially to build a functional PIC.
259 ting of two enzymes, Glo1 and Glo2, that act sequentially to convert MG into d-lactate.
260               Cry1A insecticidal toxins bind sequentially to different larval gut proteins facilitati
261             Each switch is turned on and off sequentially to distribute and route the propagating ele
262 n Euclidean distances and can be implemented sequentially to enable analyses of data that would other
263 f two CYPs, CYP76AH3 and CYP76AK1, which act sequentially to form a bifurcating pathway for the biosy
264 ers often inspect different parts of a scene sequentially to form overall perception, suggesting that
265 ion (prior + one experiment) were determined sequentially to search towards the desired separation.
266 ated conformational changes when gp120 binds sequentially to the receptors, CD4 and CCR5, on the targ
267 y retained in early endosomes and then moves sequentially to the trans-Golgi network (TGN) and recycl
268 ults indicate that cellular starvation leads sequentially to TOR inactivation, autophagy, and BZR1 de
269 eptual learning domains, we demonstrate that sequentially training participants on tasks that share a
270 ly stored in the male reproductive tract and sequentially transferred to the female reproductive trac
271 ded design and reverse genetics were used to sequentially transplant larger portions of the DENV3-spe
272 olecular dynamics simulations of a series of sequentially truncated high-mannose IgG1 Fc glycoforms,
273 t fluorescent molecules are distinguished by sequentially turning them off (or on) using a pattern of
274 onoclonal gammopathy of unknown significance sequentially underwent dual-energy CT and magnetic reson
275 archic pathway by which distinct beta-sheets sequentially unfold using the unique possibility offered
276 e show evidence that p53 (TP53) and CBFB are sequentially up-regulated in response to RUNX1 depletion
277  with which the links in a given network are sequentially used, which we call the link order, may be
278 es), and trastuzumab adjuvant regimen (given sequentially vs concurrently with chemotherapy), then im
279 hat regions of accessibility are established sequentially, where enhancers are opened in advance of p
280 y reacting with two aptamers as 'dual locks' sequentially, which leads to cell-subtype discrimination
281 ice minicolumns (maintained at 37 degrees C) sequentially with artificial saliva, gastric juice, and
282 sion continued consolidation with dasatinib, sequentially with cytarabine, asparaginase, and methotre
283 the same inflammatory lesions were performed sequentially with different CT windows.
284       Two additional patients were diagnosed sequentially with herpes simplex keratitis, then Acantha
285 iated with various closed orbits also emerge sequentially with increasing density.
286 a a multistep process in which Isu interacts sequentially with multiple protein factors.
287 magnetic nanoparticles (Fe3O4) were modified sequentially with silica (Fe3O4@SiO2), glycidyl methacry
288 id cell subsets that load differentially and sequentially with this CTC-derived material.
289 on of pyridyl radicals with 1,3-butadiene or sequentially with two acetylene molecules, respectively.
290                 Regression models were built sequentially with variables conceptually grouped accordi
291 ss deconstruction occurs synergistically and sequentially, with hemicellulose being degraded preferen
292 ind that H2A-H2B histone dimers are released sequentially, with the first dimer being released after
293         We found that neurons were activated sequentially, with the sequences spanning the entire del
294                 Regression models were built sequentially, with variables conceptually grouped accord
295 -FES and (18)F-4FMFES PET/CT scans were done sequentially (within a week) and in random order.
296  environment yet allows users to run samples sequentially without a cluster.
297 protrusions to form closely spaced adhesions sequentially without having to skip over gaps in adhesiv
298 s, in which the two BHJ layers are spin cast sequentially without the need of adopting a middle elect
299 ch use 2 assays designed as first-tier tests sequentially, without the need of Western blots.
300 y to two regulatory sites per dimer to form, sequentially, Zur2:Zn3 and Zur2:Zn4 forms of the repress

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