ライフサイエンスコーパス: serotyping

1 isolates tested in comparison to traditional serotyping.

2 reatly reduce our reliance upon conventional serotyping.

3 ts in complete concordance with conventional serotyping.

4 rphism in cps genes and correlates well with serotyping.

5 on reference laboratory for confirmation and serotyping.

6 ting ability of PFGE was better than that of serotyping.

7 ), rapid amplified polymorphic DNA, and Lior serotyping.

8 e a simple and stable surrogate for capsular serotyping.

9 pulsed-field gel electrophoresis (PFGE) and serotyping.

10 There are several methods available for serotyping.

11 cificity versus results of standard Quellung serotyping.

12 ns were also characterized by auxotyping and serotyping.

13 ods were used for pneumococcus isolation and serotyping.

14 serovars of H. parasuis, for rapid molecular serotyping.

15 FGE), multilocus sequence typing (MLST), and serotyping.

16 l composition forms the basis for Salmonella serotyping.

17 ) and pertussis toxin (ptxA) genotyping, and serotyping.

18 E, 99.7%; MEE, 99.4%; ribotyping, 98.8%; MAb serotyping, 75.8%; MAb serotyping and/or serosubtyping 9

19 resis, pulsed-field gel electrophoresis, and serotyping, 84% (52/62) of the isolates available for st

20 Although molecular serotyping added 7% (737/11,224) serotyping data, the in

21 lationship could not have been determined by serotyping alone (P = 0.320), demonstrating the value of

22 om amplified polymeric DNA (RAPD) and Penner serotyping analyses.

23 2016, 13 468 (94.9%) were characterized with serotyping and 12 235 (86.2%) with antibiotic susceptibi

24 uality control (QC) program for pneumococcal serotyping and antibiotic susceptibility testing was inc

25 Serotyping and antibiotic susceptibility were performed

26 Serotyping and antibiotic susceptibility were performed

27 demonstrate a high degree of correlation of serotyping and antimicrobial susceptibility testing resu

28 As part of ongoing national surveillance, serotyping and antimicrobial susceptibility testing were

29 Pneumococcal serotyping and antimicrobial susceptibility testing were

30 monocytogenes isolates were characterized by serotyping and automated EcoRI ribotyping.

31 To improve sensitivity and specificity of serotyping and for early detection, a multiplex PCR assa

32 e data from PCR serotyping with conventional serotyping and found that PCR serotyping was nearly as d

33 t the first distribution using their current serotyping and genotyping methods.

34 lar techniques were more discriminatory than serotyping and identified differences between some isola

35 L. monocytogenes is usually characterized by serotyping and is subtyped by using pulsed-field gel ele

36 emonstrate concordance between real-time PCR serotyping and latex agglutination.

37 break-associated cases were identified using serotyping and molecular subtyping procedures.

38 Serotyping and molecular typing by pulsed-field gel elec

39 Meningococcal isolates were identified by serotyping and multilocus enzyme electrophoresis.

40 Serotyping and other phenotypic methods are often used t

41 vestigations and could complement or replace serotyping and other subtyping methods.

42 %) had concordant results between phenotypic serotyping and PCR.

43 While serotyping and phage typing have been used widely to cha

44 ted a genetic linkage analysis that included serotyping and phenotyping 421 individuals from 39 famil

45 water-borne or food-borne diarrhea, such as serotyping and pulsed-field electrophoresis.

46 y, and the results were compared to those of serotyping and pulsed-field gel electrophoresis (PFGE).

47 Serotyping and pulsed-field gel electrophoresis were per

48 inical isolates of Listeria monocytogenes by serotyping and restriction fragment length polymorphism

49 Serotyping and serogrouping of Salmonella isolates was p

50 ater discriminatory abilities than MAb-based serotyping and serosubtyping or ITS PCR-RFLP.

51 ive methods: monoclonal antibody (MAb)-based serotyping and serosubtyping, DNA macrorestriction analy

52 ied polymorphic DNA assay (two primers), and serotyping and serosubtyping.

53 and serosubtype determination) by MAb-based serotyping and serosubtyping.

54 Serotyping and susceptibility testing of isolates were p

55 Serotyping and susceptibility testing of isolates were p

56 Isolates underwent serotyping and susceptibility testing.

57 al and pleural fluid) and were available for serotyping and susceptibility testing.

58 ns the extensive cross-reactivity based upon serotyping and the lack of consistent association of giv

59 asopharyngeal isolates were characterized by serotyping and whole-genome sequencing.

60 typing of Salmonella is better than that of serotyping and/or PFGE typing.

61 ibotyping, 98.8%; MAb serotyping, 75.8%; MAb serotyping and/or serosubtyping 97.5%; and ITS PCR-RFLP,

62 loci distinguished by serological reactions (serotyping) and by differences in the amino acid sequenc

63 strains undistinguishable by PFGE typing and serotyping), and it may be of value during investigation

64 entiated several strains undifferentiable by serotyping, and 78 distinct PFGE types were identified a

65 Pulsed-field gel electrophoresis (PFGE), serotyping, and antibiotic susceptibility testing were p

66 Automated blood culture, manual speciation, serotyping, and antimicrobial susceptibility testing wer

67 by pulsed-field gel electrophoresis (PFGE), serotyping, and antimicrobial susceptibility testing.

68 ut different from the results of traditional serotyping, and five isolates (1.3%) generated false-neg

69 ence genotyping, hemagglutination testing, O serotyping, and PCR-based phylotyping.

70 ased surveillance associated with gonococcal serotyping, and provides useful information about the mo

71 ility, pneumococcal surface protein A (PspA) serotyping, and pulsed-field gel electrophoresis (PFGE)

72 es were indistinguishable by PFGE, somatic O serotyping, and sequencing of the 582-bp region of the f

73 The rapid isolation, serotyping, and vaccine matching of FMD virus from disea

74 oung asymptomatic carriers in Chile, we used serotyping, antibiotic susceptibility testing, and genot

75 ary 2003 and June 2004 were characterized by serotyping, antibiotic susceptibility testing, and pulse

76 ins PIA and PIB of Neisseria gonorrhoeae are serotyping antigens for the serovar classification syste

77 The isolates were characterized by serotyping, antimicrobial-susceptibility testing, phage

78 commercial Salmonella somatic and flagellar serotyping antisera to in-house-prepared antisera from t

79 To develop a PCR- and sequence-based serotyping approach, we integrated available data source

80 As the development of molecular serotyping approaches is critical for Salmonella spp., w

81 22/50 [16 to 44%]), and the three different serotyping approaches that used broth enrichment increas

82 The multiplex serotyping assay consists of 24 assays specific for 36 s

83 The specificity of the serotyping assay is fully characterized with pneumococci

84 es the need to validate any new pneumococcal serotyping assay with a large number of clinical isolate

85 lysate preparation protocol and a multiplex serotyping assay.

86 Advantages over other serotyping assays are described.

87 test were sent for confirmatory testing and serotyping at the Hinton State Laboratory Institute (HSL

88 Compared to serotyping, both tests had 96% sensitivity; specificity

89 as pulsed-field gel electrophoresis and O:H serotyping but more convenient.

90 t a level comparable to that of conventional serotyping, but at a fraction of both the cost and time

91 ate, could be used as a simple surrogate for serotyping by clinical microbiology laboratories that ar

92 Molecular serotyping by microarray was used to determine pneumococ

93 re were presumed to be pneumococci underwent serotyping, capsular staining, pulsed-field gel electrop

94 This system should be very useful in serotyping clinical isolates for evaluating pneumococcal

95 his TAC method yields fast and comprehensive serotyping compared to the standard method and may be us

96 Repeated serotyping confirmed only 69.0% of isolates.

97 Our results show that comparable serotyping data can be obtained in different laboratorie

98 Serotyping data showed an incidence rate of 51.2% for no

99 nes isolates agreed with slide agglutination serotyping data, and 100 previously uncharacterized isol

100 h molecular serotyping added 7% (737/11,224) serotyping data, the inability to resolve 40% of samples

101 veillance and disease control in addition to serotyping data.

102 Serotyping dengue virus (DENV) from suspect human specim

103 ted for the C-prM protocol for detecting and serotyping dengue viruses.

104 cribes SerotypeFinder, an essential guide to serotyping E. coli in the 21st century.

105 Rep-PCR typing uncovered several historical serotyping errors and provided presumptive serotype assi

106 al pathogenic E. coli (ExPEC) and superseded serotyping for certain isolates with ambiguous or non-H7

107 he inhibition ELISA method will be useful in serotyping for epidemiological studies, assessing virule

108 gical grouping, T-agglutination pattern, and serotyping for M protein or opacity factor.

109 Serotyping for myositis-specific/myositis-associated aut

110 Serotyping forms the basis of national and international

111 al swabs for pneumococcal identification and serotyping from residents of all ages at 8 rural village

112 However, serotyping generally requires either latex agglutination

113 a general design strategy for detecting and serotyping genetically diverse viruses.

114 This molecular serotyping/genotyping scheme is well suited to rapid cha

115 A pneumococcal serotyping/genotyping system (PSGS) was developed based

116 ion could be a valid and reliable method for serotyping H. influenzae if the test was performed corre

117 Although serotyping has been a useful tool, this method can be in

118 Serotyping has been of great value in understanding the

119 Serotyping has been used in the past to assist epidemiol

120 ccessible, and more recently developments in serotyping have focused on molecular techniques.

121 ce analyses of rep-PCR and AFLP with somatic serotyping indicate that, in general, somatic serotyping

122 Serotyping indicated that 12 of 15 cases tested were cau

123 erotyping indicate that, in general, somatic serotyping is a poor indicator of genetic relatedness am

124 Serotyping is a universally accepted subtyping method fo

125 rol of the hundreds of antisera required for serotyping is difficult and time-consuming.

126 Accurate serotyping is essential to monitor the changes in the se

127 it is useful for a laboratory where standard serotyping is not available.

128 Antibody-based serotyping is unworkable when specimens are urine or vag

129 types of data for DQA1 and DRB1 showed that serotyping led to generally lower estimates of S.

130 (DBCT) identification method with Lancefield serotyping (LS).

131 Here, we employed a novel serotyping method and NMR spectroscopy to examine clinic

132 have recently developed a rapid pneumococcal serotyping method called "multibead assay" based on a mu

133 it the role of this gene in a sequence-based serotyping method.

134 resents an attractive alternative to current serotyping methods and may allow for improved acquisitio

135 Traditional serotyping methods are cumbersome and insufficient for d

136 Conventional serotyping methods are laborious and expensive.

137 era and highlight the idea that conventional serotyping methods are not capable of recognizing all pu

138 Conventional serotyping methods assume that each serotype is a geneti

139 Because classic pneumococcal serotyping methods cannot distinguish between serotypes

140 the identification of the best pneumococcal serotyping methods for carriage studies.

141 hat can help with the selection of molecular serotyping methods to be used by different laboratories.

142 Conventional serotyping methods using rabbit polyclonal or mouse mono

143 is that not all of the available alternative serotyping methods were included.

144 Twenty pneumococcal serotyping methods were used to test 81 laboratory-prepa

145 For the alternate serotyping methods, the overall sensitivity ranged from

146 isolates tested in parallel with traditional serotyping methods.

147 Using serotyping, multilocus sequence typing, and whole-genome

148 Serotyping, multiple high-resolution molecular genotypin

149 ethods and the reference method (traditional serotyping of >100 colonies from each sample).

150 HLA class I serotyping of 158 chronically infected patients revealed

151 Serotyping of 178 patient isolates revealed that 34% had

152 ELISA serotyping of 89 of 101 L. monocytogenes isolates agreed

153 Serotyping of Actinobacillus pleuropneumoniae is based o

154 mponent of the thermostable antigen used for serotyping of C. jejuni.

155 A method is described for the serotyping of Cryptococcus neoformans based on direct an

156 Serotyping of DV is accomplished by a judicious design o

157 lent mismatch discrimination ability permits serotyping of DVs in one tube with excellent sensitivity

158 n ultrasensitive assay for the detection and serotyping of DVs is described in the report.

159 Serotyping of GBS isolates is important for understandin

160 for the rapid detection, identification, and serotyping of human adenoviruses.

161 clinical laboratory isolation, and continued serotyping of isolates in public health laboratories.

162 We confirmed serotyping of isolates with sequencing of wcjE alleles.

163 ed incidence rates and adjusted rate ratios, serotyping of isolates, and comparison of secular trends

164 oratory (Baltimore, MD) for confirmation and serotyping of S. flexneri; one-third of isolates were se

165 Serotyping of Salmonella has been an invaluable subtypin

166 Isolation and serotyping of Salmonella were performed according to the

167 advances in the molecular identification and serotyping of Streptococcus pneumoniae are useful for cu

168 Accurate serotyping of Streptococcus pneumoniae remains important

169 iable, a feature used for identification and serotyping of various GAS strains.

170 HLA-A and HLA-B serotyping on URD was provided by the registries.

171 ing Vibrio parahaemolyticus strains involves serotyping or detection methods based on assessment of t

172 ledge about its genetic diversity comes from serotyping or ompA genotyping, no quantitative assessmen

173 Serotyping PCR primers were designed from variable regio

174 We used O:K:H;F serotyping, PCR-based genomic fingerprinting, pulsed-fie

175 The methods employed were somatic O serotyping, PCR-restriction fragment length polymorphism

176 Commonly used subtyping methods, such as serotyping, phage typing, ribotyping, and pulsed-field g

177 nd provides a national reference service for serotyping pneumococcal isolates in England and Wales.

178 In addition to serotyping, pneumococcal isolates causing meningitis wer

179 The technical difficulties of serotyping, primarily in antiserum production and qualit

180 as described recently, identification of PCR serotyping primers would further increase the ease and a

181 efficient enzyme-linked immunosorbent assay serotyping protocol was described recently, identificati

182 lly related pharyngeal isolates by M protein serotyping, pulsed field gel electrophoresis (PFGE), and

183 monocytogenes strains were characterized by serotyping, pulsed-field gel electrophoresis (PFGE), and

184 m in the United States were characterized by serotyping, pulsed-field restriction analysis, ribotypin

185 However, serotyping reagents are not widely available, and many i

186 yped, and some may cross-react with multiple serotyping reagents.

187 35B, one isolate failed to bind to critical serotyping reagents.

188 Here we report, from the 15 serotyping reference strains, the DNA sequences of the l

189 o led to replacement with new strains; thus, serotyping remains important for vaccine-related disease

190 We propose that real-time PCR serotyping represents an attractive alternative to curre

191 ontrast to Ab responses to whole flagella (H serotyping), responses to flagellin monomers displayed o

192 served complete concordance between capsular serotyping results and wciP pyrosequencing among 210 iso

193 , rendering them dysfunctional; inconclusive serotyping results due to nonspecific cross-reactions; o

194 Serotyping results were consistent between latex aggluti

195 ere analyzed by mPCR and compared to the IHA serotyping results with 90% concordance (excluding those

196 luster analysis and compared to conventional serotyping results.

197 or certain isolates with ambiguous or non-H7 serotyping results.

198 es not included in the PSGS yielded negative serotyping results.

199 sis and thus likely represent false-positive serotyping results.

200 Serotyping revealed that 16 of 18 ABPA patients were eit

201 is study was to evaluate a real-time PCR for serotyping S. flexneri and to use whole-genome sequencin

202 resolve discrepancies in slide agglutination serotyping (SAST) results from state health departments

203 e biotyped, serotyped by slide agglutination serotyping (SAST), and evaluated using PCR capsule typin

204 We describe an adaptation of the Penner serotyping scheme in which passive hemagglutination has

205 taining the integrity of the Kauffmann-White serotyping scheme, thus providing backwards-compatible e

206 i is the major serodeterminant of the Penner serotyping scheme.

207 igens (fliC and fljB) of the Kauffmann-White serotyping scheme.

208 fied antigenic determinants for serogroup 11 serotyping sera and highlight the idea that conventional

209 We also show that serotyping sera and monoclonal antibodies specific for 9

210 We examined the binding of serotyping sera to serogroup 11 polysaccharides by using

211 A combination of ribotyping and serotyping showed that two bovine isolates were indistin

212 r laboratories quality reagents suitable for serotyping strains of salmonellae.

213 Serotyping Streptococcus pneumoniae is a technique gener

214 In the course of large-scale serotyping studies in which fluorescent antibody stainin

215 previously devised monoclonal antibody-based serotyping system (346 CS6As compared).

216 Our serotyping system can identify not only all the serotype

217 antigenic component of the classical Penner serotyping system distinguishing Campylobacter into >60

218 acterized a rapid semiautomated pneumococcal serotyping system incorporating a pneumococcal lysate pr

219 sibility of developing an improved molecular serotyping system, which would greatly assist diagnosis

220 Serotyping the organism is important in studying the epi

221 In comparison with conventional F serotyping, the assay was extremely sensitive and specif

222 olates have traditionally been classified by serotyping, the serologic identification of two surface

223 exneri WGS data provided both genome-derived serotyping, thus supporting backward compatibility with

224 e used multilocus sequence typing (MLST) and serotyping to build a phylogenetic framework for pneumoc

225 polymerase chain reaction-based assays and O serotyping to define ExPEC-associated traits were perfor

226 rapid, sensitive, and specific screening and serotyping tools for epidemiological studies of dengue v

227 Traditional methods of serotyping using culture and serum neutralization are ti

228 outine analysis of Listeria monocytogenes by serotyping using traditional agglutination methods is li

229 According to serotyping, virulence genotyping, and random amplified p

230 fied by UAD and by Quellung reaction and PCR serotyping was 70/86 (81.4%).

231 h conventional serotyping and found that PCR serotyping was nearly as discriminatory as conventional

232 Since viral titers were too low, complete serotyping was not possible.

233 was nearly as discriminatory as conventional serotyping was.

234 gens in the United States, serosubtyping and serotyping were done on 444 NMSB strains isolated in the

235 n the well-established utility of Salmonella serotyping when integrated into a platform of WGS-based

236 nzyme-linked immunosorbent assay (ELISA) for serotyping which is sensitive and specific compared to t

237 In addition to serotyping, which is subject to false-positive results,

238 We compared the data from PCR serotyping with conventional serotyping and found that P

239 59 [85.5%] of 69 isolates) was identified by serotyping (with 85.5% of the isolates being 19A or 19F)

240 This method will find utility in high-volume serotyping work.

241 g sera and expertise needed for conventional serotyping yet has the modest equipment necessary for DN