ライフサイエンスコーパス: serum-free medium

1 nonadjuvanted, isologous mAbs purified from serum-free medium.

2 e metacarpophalangeal joints was cultured in serum-free medium.

3 optosis mediated by the free RGDS peptide or serum-free medium.

4 cell aggregates after exposure to a defined serum-free medium.

5 d RACK1 levels and promoted cell survival in serum-free medium.

6 c) in a number of insect cell lines grown in serum-free medium.

7 mRNAs were detectable in HCjE cells grown in serum-free medium.

8 ls are severely reduced in their growth in a serum-free medium.

9 etal or rat neonatal cortex were cultured in serum-free medium.

10 tic activity of NPM/ALK in growth factor and serum-free medium.

11 pared with control cells, when cultured in a serum-free medium.

12 alpha (TNF-alpha) was added at 10 ng/ml to a serum-free medium.

13 was blocked by the MEK inhibitor PD98059 in serum-free medium.

14 al human conjunctival fibroblasts (HJF) in a serum-free medium.

15 eir nonstimulated counterparts cultured in a serum-free medium.

16 ng as 107 days in alginate in a supplemented serum-free medium.

17 se no effect was seen in cells cultured with serum-free medium.

18 wild-type mice were avulsed and cultured in serum-free medium.

19 induced rat granulosa cytodifferentiation in serum-free medium.

20 factor (50 ng/mL), and PIXY321 (50 ng/mL) in serum-free medium.

21 ls and show that these cells can now grow in serum-free medium.

22 the proliferation of astrocytes raised in a serum-free medium.

23 sa association with both substrates, only in serum-free medium.

24 ctions from AD as well as non-AD patients in serum-free medium.

25 endo constitutively secrete PDGF activity in serum-free medium.

26 in kinase (MAPK) activity and proliferate in serum-free medium.

27 ell number even when the cells were grown in serum-free medium.

28 ls but also supported their proliferation in serum-free medium.

29 hibited by all trans-retinoic acid (ATRA) in serum-free medium.

30 tiated into skeletal muscle when cultured in serum-free medium.

31 aline-like translucent cartilage particle in serum-free medium.

32 secretes IgMkappa light chain antibody in a serum-free medium.

33 m embryonic mouse OE and cultured in defined serum-free medium.

34 inability to grow in semisolid medium or in serum-free medium.

35 onto serum-coated or noncoated substrata in serum-free medium.

36 cells form neurospheres when transferred to serum-free medium.

37 t neurospheres in growth factor supplemented serum-free medium.

38 land epithelial cells and to culture them in serum-free medium.

39 um treated with methyl-beta-cyclodextrin, or serum-free medium.

40 enosine nucleotides cannot support growth in serum-free medium.

41 were cultured in high-density monolayers in serum-free medium.

42 rabbit corneal keratocytes were cultured in serum-free medium.

43 posure to IL-1beta with or without NS-398 in serum-free medium.

44 nd keratocan) were expressed and secreted in serum-free medium.

45 an astrocyte feeder layer and maintained in serum-free medium.

46 x 10(11)M) with or without NS-398 (10 nM) in serum-free medium.

47 ((3)H-adenosine) in low-physiologic-glucose serum-free medium.

48 ccumulation, promoting cell proliferation in serum-free medium.

49 he antiproliferative effects of rapamycin in serum-free medium.

50 ), and fibroblast growth factor-1 (FGF-1) in serum-free medium.

51 All BHK cell lines secreted r-hFIX into serum-free medium.

52 /cm2) and high (20,000 cells/cm2) density in serum-free medium.

53 division (P = 0.01), increased migration in serum-free medium (72 +/- 18 migrated cells versus 33 +/

54 In cardiac myocytes cultured in serum and in serum-free medium, a myc-tagged Vgl-4 protein was locate

55 The model uses serum-free medium, a nonbiological substrate N-1[3(trime

56 In serum-free medium, abnormal epidermal-like differentiati

57 ir native collagen capsule and maintained in serum-free medium actively grow and thus show an intrins

58 +) cells, Dox(-)cells, or an equal volume of serum-free medium after surgically induced myocardial in

59 Single cell plating in serum-free medium allows direct assessment of growth fac

60 /ml) or activated TGF-beta1 (1 ng/ml), or in serum-free medium alone (untreated control samples).

61 Compared with serum-free medium alone, BAL fluid obtained from normal

62 thus containing a mixture of them all, or in serum-free medium alone.

63 -derived stem cells to naive pluripotency in serum-free medium alone.

64 ostate development, fetal UGSs were grown in serum-free medium and 5 alpha dihydrotestosterone (DHT)

65 ptides prepared from M. arthritidis grown in serum-free medium and also from a 41-kDa known bioactive

66 xclusion) are first noted at 50 microM EB in serum-free medium and at > or = 200 microM in the presen

67 m these cells during extended incubations in serum-free medium and at different stages of dedifferent

68 vity increased during extended incubation in serum-free medium and during myofibroblastic dedifferent

69 uman monocytes were cultured for 24 hours in serum-free medium and granulocyte-macrophage colony-stim

70 blood and placed in culture wells containing serum-free medium and growth factors.

71 ll lung cancer (NSCLC) cell lines to ATRA in serum-free medium and in medium supplemented with delipi

72 and cantilever substrates with a DETA SAM, a serum-free medium and refined culture techniques.

73 transfectants proliferated when switched to serum-free medium and regained rapid growth when serum w

74 ed chondrocytes were cultured in alginate in serum-free medium and stimulated with IGF-1 or des(1-3)

75 HCLE cells were grown to confluence in serum-free medium and switched to DMEM/F12 with 10% seru

76 as the spherogenicity of single CRC cells in serum-free medium and the size of the side population (S

77 s (n = 7) were cultured in alginate beads in serum-free medium and treated for 21 days with 100 ng/ml

78 HCECs were cultured in serum-free medium and treated with 0 or 10 ng/mL TGFB1 o

79 Transformed RGC-5 cells were cultured in serum-free medium and were treated with 0.5 muM to 2.0 m

80 n-aggressive breast cancer cells cultured in serum-free medium and which appear to be necessary for p

81 in-exposed slices (10 microg ml(-1) added to serum-free medium), and between neurones in slices deriv

82 eatment with HES1 shRNA, cell aggregation in serum-free medium, and a mixture of soluble factors furt

83 ypoxia (3% O(2)) or normoxia (18% O(2)) in a serum-free medium, and cell proliferation as well as the

84 ibroblastic cells was replaced on day 4 with serum-free medium, and cells were cultured until day 12.

85 It induced newt myotubes to enter S phase in serum-free medium, and it acted on myotubes but not on t

86 tein stimulated the growth of HepG2 cells in serum-free medium, and partially protected cells from an

87 Keratocytes cultured in serum-free medium appeared dendritic and became fibrobla

88 ever, after culture of monocytes for 48 h in serum-free medium, approximately 30% of the resulting ma

89 P < 0.001), respectively, when compared with serum-free medium as control.

90 of cells differentiating into macrophages in serum-free medium, as assessed by expression of the alph

91 Addition of recombinant human IGF-II to the serum-free medium blocked both cell death and colony reg

92 In serum-free medium, BMP-2 induced significantly less Smad

93 Cyr61 regulates gene expression not only in serum-free medium but also in fibroblasts cultured on va

94 o keratinocyte-derived SCC12 cells, grown in serum-free medium but exposed to fibronectin, suppressed

95 in-1 (AP-1)-luciferase reporter construct in serum-free medium but not in delipidized serum.

96 so, stimulated the growth of Calu-1 cells in serum-free medium but not in serum-containing medium.

97 HMC ADAM 15 was expressed at low levels in serum-free medium but was increased during migration.

98 dendroglial differentiation when cultured in serum-free medium, but differentiate into astrocytes in

99 in serum-containing medium and on plastic in serum-free medium, but expression was lost on plastic in

100 bit NK cell-mediated natural cytotoxicity in serum-free medium, but had not been shown to inhibit ant

101 eration or increase survival of RPE cells in serum-free medium, but promoted a differentiated morphol

102 s stably overexpressing Mirk proliferated in serum-free medium, but the mechanism of Mirk action is u

103 henotype can be maintained in a low-calcium, serum-free medium by downregulating Smad-mediated TGF-be

104 0 and Rdh16 mRNA in HepG2 cells incubated in serum-free medium by inhibiting transcription and destab

105 When these cells were adapted to grow in the serum-free medium (CD18/HPAF-SF), the MUC4 expression wa

106 In serum-free medium, CD34 expression was maintained by cel

107 ion, and these cells grew more vigorously in serum-free medium compared to control-transfected cells.

108 The survival-promoting properties of serum-free medium conditioned by mesangial cells was abr

109 I RIA demonstrated secreted IGF-I protein in serum-free medium conditioned by the IGF-I-expressing ce

110 Serum-free medium conditioned by V12H10 cells contains a

111 ltured on fibronectin-coated dishes in QB-58 serum-free medium containing 20 microliters/ml bovine re

112 orts, we find that when SMNs are cultured in serum-free medium containing a single peptide trophic fa

113 by substratum-independent pellet culture in serum-free medium containing ascorbate.

114 plated 1 cell per well in Terasaki plates in serum-free medium containing cytokines.

115 llar granule cells can also be maintained in serum-free medium containing either 100 ng/ml insulin-li

116 granule neurons grown in chemically defined, serum-free medium containing either nondepolarizing (5 m

117 c-kit+Lin- cells were cultured for 9 days in serum-free medium containing interleukin (IL)-6, IL-11,

118 two-step panning method and were cultured in serum-free medium containing neurotrophic factors and fo

119 stnatal rat optic nerve and cultured them in serum-free medium containing platelet-derived growth fac

120 tured at a central cell-processing center in serum-free medium containing rIL-2 to generate TILs.

121 A serum-free medium containing SCF, TPO, and FGF-1 or Flt3

122 cultured as micromass pellets for 21 days in serum-free medium containing transforming growth factor

123 Semiconfluent LNCaP cells were grown in serum-free medium containing varying concentrations of t

124 Rat aortic ECs (n=30) or serum-free medium (controls; n=29) were seeded endovascu

125 After challenge in serum free medium, CPCs treated with the 3 microRNA cock

126 Twenty-four hours after plating in serum-free medium, cultures were exposed to DHEA, DHEAS,

127 beta transforming growth factor (TGFbeta) in serum-free medium decreased levels of p15(INK4B) and inc

128 m of hyaluronans and Kubota's medium (KM), a serum-free medium designed for endodermal stem/progenito

129 T. denticola grown in a serum-free medium did not exhibit increased susceptibili

130 Survival of PC12 cells in serum-free medium did not require activity of the ras/er

131 Subsequent culture of the fibroblasts in serum-free medium did not restore aldehyde dehydrogenase

132 We have propagated, in serum-free medium, epithelial cell cultures derived from

133 istilled water (EtOH-H2O) or to keratinocyte serum-free medium (EtOH-KSFM) for incubation periods of

134 Over more than 3 weeks, cells grown in serum-free medium expanded more than 800,000-fold, and 8

135 34+ hematopoietic progenitor cells in liquid serum-free medium favoring growth of erythroid precursor

136 cultures variant fibrinogen accumulation in serum-free medium fluctuated between 1 and 15 micrograms

137 d on polylysine/laminin-coated coverslips in serum-free medium for 2 days.

138 were exposed to Ag-pulsed accessory cells in serum-free medium for 24 h; cultured in the absence of A

139 Confluent CMECs were exposed to IL-1 in serum-free medium for 24 hours, and cell-conditioned med

140 tes were incubated in an amino acid-free and serum-free medium for 3 hours prior to onset of anoxia.

141 endothelial cells (bAECs) were incubated in serum-free medium for 6 h before addition of 50 nmol/l f

142 t metatarsal bones (dpc 20) were cultured in serum-free medium for 7 days in the presence or absence

143 6 month) rat hippocampal neurons cultured in serum-free medium for 7-12 days.

144 Aortic rings from rats were incubated in serum-free medium for 9 d, and calcification was measure

145 ival were found in murine islets cultured in serum-free medium for 96 hr with 500 ng/ml NGF (P<0.05).

146 of sEVs secretion from TK6 cells cultured in serum-free medium for a culturing period from 1 to 48 h.

147 n and differential adhesion, and cultured in serum-free medium for approximately 2 days on glass cove

148 re isolated from human tracheas and grown in serum-free medium for one week.

149 oratory successfully demonstrated that using serum-free medium for prolonged pancreatic islet culture

150 transfected with AAC-11 cDNA were viable in serum-free medium for up to 12 weeks.

151 even when 10 ng/ml TGF-beta1 was added in a serum-free medium for up to 5 days.

152 Retinal cells were cultured in serum-free medium for up to 6 days in the presence of va

153 CD133+ FACS-sorted cells cultured in serum-free medium form 3-fold more neurospheres than do

154 ed cell cycle entry by >5-fold compared with serum-free medium (from 13.5 to 78%), but at the single

155 When placed in serum-free medium, granulocytes and affected CD34(+) (CD

156 and 39 + or - 13% in the Dox(+), Dox(-), and serum-free medium groups, respectively (P<0.05 for the d

157 After incubating the cells for 13 days in serum-free medium in 96-well microplates, there was a st

158 cells within six CRC lines form spheroids in serum-free medium in suspension.

159 orescence protein mice and grown for 48 h in serum-free medium in the presence or absence of Ang II.

160 NA levels in neuroblastoma cells cultured in serum-free medium increased after 8 to 16 hours in serum

161 The addition of exogenous FA in serum-free medium increased oxLDL binding and uptake to

162 s apoptosis in sup-II preneoplastic cells in serum-free medium, indicating that c-Fos protein can ind

163 Mechanical activation of mTOR occurred in serum-free medium, indicating that it is independent of

164 TGF-beta1 alone or combined with PDGF-BB in serum-free medium induces a temporally correct expressio

165 Proliferation of U373 MG cells in serum-free medium is inhibited by EGF.

166 ICE-mediated apoptosis of COS cells in serum-free medium is suppressed by insulin-like growth f

167 eted factor has been partially purified from serum-free medium, is protease-sensitive, and has a mole

168 containing medium or in a chemically defined serum-free medium (ITS).

169 inar-like phenotype when the [Ca(2+)] in the serum-free medium (keratinocyte growth medium, KGM) was

170 ase II with sorbitol in defined keratinocyte serum-free medium (KSFM) or supplementary hormonal epith

171 (DMEM/10% FBS), or in a defined keratinocyte serum-free medium (KSFM).

172 ne whether culturing of islets in a modified serum-free medium (M-SFM) would sustain function in vivo

173 In serum-free medium, most antibiotics (except polymyxins)

174 ncompatible 3F7.A10 hybridoma cells grown in serum-free medium mounted strong anti-Id responses.

175 were added individually to cells growing in serum-free medium next to controls in medium supplemente

176 This set-up included use of serum-free medium of defined composition with supplement

177 uman epidermal keratinocytes (NHEK) grown in serum-free medium on a plastic substrate spontaneously d

178 ther alone or in combination was examined in serum-free medium on canine aortic SMCs by [3H]thymidine

179 eratinocytes (HFKs) cultured in keratinocyte serum-free medium on plastic senesced at approximately 1

180 individual and combinations of cytokines in serum-free medium on the kinetics of the first cell doub

181 oblasts (HCFs) were cultured in supplemented serum-free medium on VN or collagen (CL) with 1 ng/mL tr

182 Exposure of 3T3/A31 cells to serum-free medium, one type of apoptotic stimulus, cause

183 In serum-free medium, one-third of RA-treated cells become

184 romoted effects were strikingly different in serum-free medium or in the presence of the tyrosine kin

185 However, when they are starved in serum-free medium, ovarian cancer cells ceased producing

186 e more than twofold higher in omental cells (serum-free medium: P < 0.05; TNF-alpha: P < 0.02; paired

187 rom rabbit corneal stroma, and cultured in a serum-free medium, pretreated or not treated with JNK in

188 Suspension cultures of L-33(+) cells in serum-free medium produce HSPGact and secrete HSact conv

189 hed to confluent monolayers of A549 cells in serum-free medium, reaching a plateau within 40 min.

190 18 fatty acid synthesis in cells cultured in serum-free medium renders them resistant to killing by 2

191 Addition of recombinant Gas-6 to serum-free medium resulted in increased survival of prim

192 A serum-free medium revealed two effects of TNFalpha: (1)

193 ancer cell line MDA MB 231 (231) cultured in serum-free medium secretes transforming growth factors t

194 s were maintained in various combinations of serum-free medium, serum-free medium that was conditione

195 ically reduced when cells were maintained in serum free medium (SFM).

196 CCs but not BECs, continued to grow in basal serum-free medium (SFM) and spontaneously produced both

197 tton was cultured for 24, 48, or 72 hours in serum-free medium (SFM) or SFM supplemented with 10% fet

198 inal ganglia, and brainstem were cultured in serum-free medium (SFM) or SFM supplemented with NGF or

199 ormed by cells cultured with FBS switched to serum-free medium (SFM) was considerably more extensive.

200 fibronectin, whereas the cells incubated in serum-free medium showed only minimal immunoreactivity.

201 , which do not normally undergo apoptosis in serum-free medium, showed apoptotic DNA fragmentation up

202 cell growth assays were conducted in defined serum-free medium, spermine inhibited the growth of poor

203 Commercially prepared VN added to serum-free medium stimulated ROS phagocytosis in a dose-

204 /IR cells, they become capable of growing in serum-free medium supplemented solely with insulin or IG

205 on (HBSS), and then cultured for 72 hours in serum-free medium supplemented with 0.2% bovine serum al

206 Nanog enables somatic cell reprogramming in serum-free medium supplemented with LIF, a culture condi

207 These cells fail to proliferate in serum-free medium supplemented with purified growth fact

208 When grown in a serum-free medium supplemented with starch, M. arthritid

209 In serum-free medium, T and DHT at concentrations of 5 x 10

210 They clonogenically expand on plastic and in serum-free medium, tailored for endodermal progenitors,

211 s displayed a greater capacity for growth in serum-free medium than Mes SMCs, but only under conditio

212 tant was able to grow to a higher density in serum-free medium than the wild-type strain, mimicking t

213 ent characteristics (HRA-19) and developed a serum-free medium that induces endocrine, mucous and abs

214 st-selection HMEC, that is, cells grown in a serum-free medium that overcame stasis via silencing of

215 n various combinations of serum-free medium, serum-free medium that was conditioned by neural retinas

216 m that was conditioned by neural retinas, or serum-free medium that was supplemented with bovine pitu

217 nantly high density lipoprotein), whereas in serum-free medium the [(3)H]LPS remained tightly associa

218 When added to human fibroblasts in serum-free medium, the bisphenol fluorene derivative 9,9

219 Production of the antibody in a serum-free medium, the cytotoxic potential with human co

220 wth of four cell lines was inhibited more in serum-free medium, the growth of the Calu-1 cell line wa

221 On day 7, by use of serum-free medium, the macrophages were incubated with v

222 After an additional 2 DIV in serum-free medium, the number of TH neurons had doubled,

223 In serum-free medium, the tight junctions were leaky or fai

224 er leukemic cell recoveries after culture in serum-free medium, they did not correlate with higher ce

225 Cells were cultured in serum-free medium to confluence and then cultured with e

226 rugs--ferumoxytol, heparin and protamine--in serum-free medium to form self-assembling nanocomplexes

227 moxifen or dexamethasone in phenol red-free, serum-free medium to measure the steady-state levels of

228 olyethylene glycol hydrogels and cultured in serum-free medium to model cellular interactions within

229 -human CD28 antibodies for 72 hours in AIM V serum-free medium to obtain T cell-conditioned medium, f

230 No factors were added to the serum-free medium to substitute for the ablated targets,

231 uman CB-derived CD34+ cells were cultured in serum-free medium together with SCF, TPO, FGF, with or w

232 ive (Lin-) CD34+CD38- cells were cultured in serum-free medium under 1.5% O2 (hypoxia) or 20% O2 (nor

233 ferentiate into more mature myeloid cells in serum-free medium unless IGF-I was added.

234 Serum-free medium was added to RPE eyecups (a healthy mo

235 Serum-free medium was added to these RPE eyecups, and, a

236 Furthermore, the growth of DU145 and PC3 in serum-free medium was also inhibited by anti-IL-6 antibo

237 al of epithelial cells cultured overnight in serum-free medium was promoted by adhesion, which activa

238 Cell survival was assured when serum-free medium was supplemented with any one or all o

239 CD36-mediated uptake in serum-free medium was very low but greatly increased whe

240 -epimerase activity, and Lec3 cells grown in serum-free medium were essentially devoid of sialic acid

241 Explants cultured in serum-free medium were exposed to ligands (fibronectin a

242 For these determinations, cells plated in serum-free medium were treated either with growth factor

243 d from rabbit corneal stroma and plated in a serum-free medium were treated with FGF-2/heparin or TGF

244 Cells maintained in serum-free medium were used as controls.

245 ment with transforming growth factor beta in serum-free medium, whereas no stimulation was observed f

246 so inhibited the growth of Hep3BX cells in a serum-free medium, which correlated with depressed level

247 sed in immortalized chondrocytes cultured in serum-free medium, while axl expression decreased.

248 clonal cell line) are treated for 30 min in serum-free medium with 10(-6) M colchicine.

249 poptotic death within 96 hr when switched to serum-free medium with 5 mM potassium.

250 Supplementation of serum-free medium with androgens resulted in dose-depend

251 unstimulated T cells which were cultured in serum-free medium with circadian clock reporter systems.

252 They grew as neurospheres in serum-free medium with epidermal growth factor and fibro

253 Further culture of myeloid cells in serum-free medium with GM-CSF and IL-4 generated cells t

254 Explants were cultured in serum-free medium with insulin, transferrin, and ovalbum

255 om collagenase A digestion were preserved in serum-free medium with low or high calcium for up to 3 w

256 These cells constitutively produce 5-HETE in serum-free medium with no added stimulus.

257 n alginate beads or as cartilage explants in serum-free medium with or without IGF-1 (100 ng/ml), OP-

258 cells were washed and incubated for 24 h in serum-free medium with or without the addition of 8-brom

259 oblasts (GF) were tested in phenol red-free, serum-free medium with or without the progestogen, medro

260 lls obtained from adult skin and cultured in serum-free medium with recombinant human stem cell facto

261 Cultures were maintained in serum-free medium with tetrodotoxin to suppress spontane

262 ndritic cells, the cultures were switched to serum-free medium with the growth factors granulocyte-ma

263 We expanded CD34(+) cells from CB in serum-free medium with thrombopoietin, flk-2 ligand, and

264 d neonatal ventricular myocytes by growth in serum-free medium with varying triiodothyronine (T3) lev

265 acrine cells were cultured at low density in serum-free medium, with and without peptide trophic fact

266 thelial cell line, N/N1003A, was cultured in serum-free medium, with or without EGF.

267 vere periodontitis patients were cultured in serum-free medium, with or without IL-1beta (10(-11)M) f

268 We incubated early-passage hMSCs in serum-free medium without cytokines or other supplements

269 IGF-II causes these cells to proliferate in serum-free medium without growth factor supplementation.

270 MMC) neurons died within 72 hr when grown in serum-free medium without trophic factors.