ライフサイエンスコーパス: sulfurylase

1 l ("PAPS synthetase") ancestor of fungal ATP sulfurylase.

2 erminal domain that is present in fungal ATP sulfurylase.

3 ress curves during the first turnover of ATP sulfurylase.

4 c differences between the two classes of ATP sulfurylase.

5 -terminal APS kinase and a COOH-terminal ATP sulfurylase.

6 code APS kinase, while exons 6-13 encode ATP sulfurylase.

7 get superoxide dismutases, laccases, and ATP sulfurylases.

8 and a carrier-free [35S]-Na2(35)SO4 with ATP sulfurylase, a recombinant APS kinase and inorganic pyro

9 Comparison of the Aquifex ATP sulfurylase active site with those from sulfate assimila

10 A slight improvement in reverse sulfurylase activity (<10% residual activity) and comple

11 o sulfurylase activity, and H506A had normal sulfurylase activity but produced an effect on kinase ac

12 By contrast, total ATP sulfurylase activity declines proportionally in all the

13 Residues specific for sulfurylase activity have also been distinguished from t

14 blated APS kinase activity while leaving ATP-sulfurylase activity intact.

15 tant role for the HXGH histidines in the ATP sulfurylase activity of bifunctional PAPS synthase and s

16 Sulfurylase activity was significantly destabilized in a

17 G59A caused a significant decrease in ATP-sulfurylase activity without effect on APS kinase activi

18 d kinase activity, R522A and R522K showed no sulfurylase activity, and H506A had normal sulfurylase a

19 e exon 6-encoded peptide showed no kinase or sulfurylase activity, demonstrating that exon 6 encodes

20 xhibited a significant (60%) loss of reverse sulfurylase activity, suggesting that this peptide regio

21 that the HXXH motif plays a role only in the sulfurylase activity, whereas the PP-loop is involved in

22 vity, whereas a 220-623 fragment evinced ATP sulfurylase activity.

23 MSK exhibited no kinase activity and reduced sulfurylase activity.

24 te as the sole sulfur source and exhibit ATP sulfurylase activity.

25 ulfurylase recombinant eventually stabilized sulfurylase activity.

26 domain of the mouse bifunctional enzyme ATP sulfurylase/adenosine 5'-phosphosulfate (APS) kinase con

27 The recently cloned murine ATP-sulfurylase/adenosine 5'-phosphosulfate (APS) kinase con

28 Mammalian ATP sulfurylase/adenosine 5'-phosphosulfate (APS) kinase con

29 ATP sulfurylase and 5'-adenylylsulfate (APS) reductase catal

30 s synthesized by the concerted action of ATP sulfurylase and adenosine 5'-phosphosulfate (APS) kinase

31 resses a gene product that exhibits both ATP sulfurylase and adenosine-5'-phosphosulfate (APS) kinase

32 lase kinase (SK) polypeptide having both ATP-sulfurylase and adenosine-phosphosulfate kinase activiti

33 t containing an adenosine triphosphate (ATP) sulfurylase and an adenosine 5'-phosphosulfate (APS) kin

34 The (a) imbalance between ATP sulfurylase and APS kinase activities, (b) accumulation

35 In simpler organisms, the ATP sulfurylase and APS kinase reactions are catalyzed by se

36 tial actions of two cytoplasmic enzymes, ATP sulfurylase and APS kinase, and then must be transferred

37 to the nature and control of the enzymes ATP sulfurylase and APS kinase, which catalyze the early ste

38 Arabidopsis plants the total activity of ATP sulfurylase and APS reductase declines by 3-fold in leav

39 ATP-sulfurylase and APS-kinase can rapidly synthesize additi

40 ity of the two enzymes of its synthesis, ATP-sulfurylase and APS-kinase.

41 emiluminescent detection of PP(i), using ATP sulfurylase and firefly luciferase, was adapted to monit

42 ity, whereas the PP-loop is involved in both sulfurylase and kinase activities.

43 The 2MSK protein possessed sulfurylase and kinase activity equivalent to the full-l

44 Of these, R510A exhibited normal sulfurylase and kinase activity, R522A and R522K showed

45 d GRD sequence of the PP-loop, affected both sulfurylase and kinase activity.

46 reversed by heterologous expression of human sulfurylase and kinase.

47 mposed of cysteine biosynthesis enzymes, ATP sulfurylase and O-acetylserine sulfhydrylase, each with

48 selenate, (b) activation of selenate by ATP sulfurylase, and (b) conversion of selenomethionine (SeM

49 unctions of this unique protein (reverse ATP-sulfurylase, APS kinase, and an overall assay) were used

50 ution X-ray crystal structure of Aquifex ATP sulfurylase-APS kinase bifunctional enzyme is presented.

51 and sequence comparison of bifunctional ATP sulfurylase/APS kinase and monofunctional ATP sulfurylas

52 ATP sulfurylase/APS kinase catalyses the metabolic activatio

53 genes, ATPSK2 and Atpsk2, encoding novel ATP sulfurylase/APS kinase orthologues in the respective reg

54 ty sulfate transporter (AST68) and three ATP sulfurylases (APS1, APS3 and APS4) in higher plants.

55 fully active in both the forward and reverse sulfurylase assays.

56 Reduced expression of ATP sulfurylase (ATPS) alone affects both sulfate translocat

57 e activity of key S assimilatory enzymes ATP sulfurylase (ATPS), APS reductase (APR), and serine acet

58 ulfate through adenylation by the enzyme ATP sulfurylase (ATPS), forming adenosine 5'-phosphosulfate

59 -affinity sulphate transporter and three ATP sulfurylases (ATPS) were the target genes of AthmiR395 (

60 merization interface compared with other ATP sulfurylases but was similar to mammalian 3'-phosphoaden

61 The distinct behavior of ATP sulfurylase can be attributed to reciprocal expression o

62 ATP sulfurylase catalyzes and couples the free energies of t

63 ur-assimilating organisms such as fungi, ATP sulfurylase catalyzes the first committed step in sulfat

64 nas reinhardtii adenosine triphosphate (ATP) sulfurylase cDNA clone (pATS1) was selected by complemen

65 e in this pathway, adenosine-5'-triphosphate sulfurylase, conferred significant protection against mu

66 mino acid sequence of the C. reinhardtii ATP sulfurylase, derived from the nucleotide sequence of the

67 kinase-like C-terminal region of fungal ATP sulfurylase does not account for the lack of APS kinase

68 Expressed protein generated from the ATP-sulfurylase domain alone was fully active in both the fo

69 The former reaction is catalyzed by the ATP-sulfurylase domain and the latter by the APS-kinase doma

70 this peptide region is interacting with the sulfurylase domain as well as functioning in the kinase

71 ) synthetase consists of a COOH-terminal ATP-sulfurylase domain covalently linked through a nonhomolo

72 phosphodiester bond of ATP, whereas the ATP sulfurylase domain involves cleavage of the alpha-beta p

73 ted mutagenesis of the HXGH motif in the ATP sulfurylase domain of human PAPS synthase (amino acids 4

74 highly conserved HXGH motif found in the ATP sulfurylase domain of PAPS synthases is involved in ATP

75 of a highly conserved HXGH motif in the ATP sulfurylase domain of PAPS synthases, a motif implicated

76 Because the ATP-sulfurylase domain of PAPS synthetase influences these e

77 The sulfurylase domain of the mouse bifunctional enzyme ATP

78 The kinetic constants of the ATP sulfurylase domain were as follows: V(max,f) = 0.77 micr

79 nd step in which APS, the product of the ATP-sulfurylase domain, is phosphorylated on its 3'-hydroxyl

80 e, chlorate, and perchlorate bind to the ATP sulfurylase domain, with the first five serving as alter

81 onserved arginines and histidines within the sulfurylase domain.

82 ATP sulfurylase domains are often embedded in multifunctiona

83 osulfate (APS) kinase consists of kinase and sulfurylase domains, and catalyzes two sequential reacti

84 chia coli cysDN genes, which code for an ATP sulfurylase (EC 2.7.7.4).

85 he analogous C-terminal region of fungal ATP sulfurylase eliminated enzyme activity.

86 ATP sulfurylase from Penicillium chrysogenum is a homohexame

87 ATP sulfurylase from Penicillium chrysogenum is an allosteri

88 ATP sulfurylase from Penicillium chrysogenum is an allosteri

89 e present here, the crystal structure of ATP sulfurylase from this bacterium at 1.7 A resolution.

90 ATP sulfurylase, from E. coli Kappa-12, is a GTPase.target c

91 ATP sulfurylase, from Escherichia coli K-12, catalyzes and c

92 ATP sulfurylase, from Escherichia coli K-12, conformationall

93 ATP sulfurylase, from Escherichia coli Kappa-12, is a GTPase

94 r chemolithotrophic bacteria, the enzyme ATP sulfurylase functions to produce ATP and inorganic sulfa

95 ected by complementing a mutation in the ATP sulfurylase gene (cysD) of Escherichia coli.

96 ct release step(s) were confirmed in the ATP sulfurylase-GTPase reaction by a burst of product in pre

97 d cleavage in the catalytic cycle of the ATP sulfurylase-GTPase, from E. coli K-12.

98 In contrast, ATP sulfurylase in sulfur chemolithotrophs catalyzes the rev

99 ATS1), is 25 to 40% identical to that of ATP sulfurylases in other eukaryotic organisms and has a put

100 lase isoform 1 from soybean (Glycine max ATP sulfurylase) in complex with APS was determined.

101 on of chlorate, a widely used cell-permeable sulfurylase inhibitor, function to reduce lithium-induce

102 by the binding of activators that drive ATP sulfurylase into forms that mimic different stages of th

103 The mechanism of ATP sulfurylase involves an enzyme isomerization that preced

104 g of mGMPPNP to the E.AMP.PPi complex of ATP sulfurylase is biphasic, indicating that an isomerizatio

105 tion of Arabidopsis leaves revealed that ATP sulfurylase isoenzymes exist in the chloroplast and the

106 reaction, the x-ray crystal structure of ATP sulfurylase isoform 1 from soybean (Glycine max ATP sulf

107 ATP sulfurylase, isolated from Escherichia coli K-12, cataly

108 ATP sulfurylase, isolated from Escherichia coli K-12, is a G

109 APS synthesis is catalyzed by a bifunctional sulfurylase kinase (SK) polypeptide having both ATP-sulf

110 respectively, renders the enzyme inactive in sulfurylase, kinase, and overall assays.

111 ast to the wild type enzyme, recombinant ATP sulfurylase lacking the C-terminal allosteric domain was

112 functional enzyme, from which the fungal ATP sulfurylase may have evolved.

113 ATP sulfurylase mRNA was present when cells were grown in su

114 ymes enhances the intrinsic stability of the sulfurylase only.

115 lanine mutants (H425A, H428A, and R421A) had sulfurylase or overall activity, whereas they all exhibi

116 Disruption of met3 or met14 genes (ATP sulfurylase or phosphosulfate kinase), transcriptional d

117 ucture and kinetic analysis suggest that ATP sulfurylase overcomes the energetic barrier of APS synth

118 otal, the results suggest that cytosolic ATP sulfurylase plays a specialized function that is probabl

119 eady-state kinetic analysis of 20 G. max ATP sulfurylase point mutants suggests a reaction mechanism

120 ase sequence at the NH2-terminal side of the sulfurylase recombinant eventually stabilized sulfurylas

121 in the fungal enzyme, the motif serves as a sulfurylase regulatory domain that binds the allosteric

122 tions in the sulfate activation pathway, ATP-sulfurylase (S) and APS-kinase (K), are fused as 'KS' in

123 ulfurylase/APS kinase and monofunctional ATP sulfurylases shows a limited number of highly conserved

124 he mechanism of energetic linkage in the ATP sulfurylase system are discussed.

125 The expression of miR395, the sulfurylase-targeting miRNA, increases upon sulfate star

126 aled that paralemmin, molybdopterin synthase sulfurylase, Tel6 oncogene (ETV6), a cleavage-specific f

127 rium was found to contain high levels of ATP sulfurylase that may provide a substantial fraction of t

128 lly linked to the chemistry catalyzed by ATP sulfurylase, the first enzyme in the cysteine biosynthet

129 ch targets three out of four isoforms of ATP sulfurylase, the first enzyme of sulfate assimilation, a

130 iption factor maintain optimal levels of ATP sulfurylase transcripts to enable increased flux through

131 ild-type plants and in selenate-supplied ATP-sulfurylase transgenic plants.

132 ctivity) and complete restoration of forward sulfurylase was observed with R421K.

133 ne, reported to be an inhibitor of brain ATP sulfurylase, was without effect on PAPS synthetase isofo

134 y-state stages of the catalytic cycle of ATP sulfurylase were studied using tools capable of distingu

135 ifex enzyme is reminiscent of the fungal ATP sulfurylase, which contains a C-terminal domain that is

136 encodes the ATPS1 isoform of the enzyme ATP sulfurylase, which precedes adenosine 5'-phosphosulfate

137 ted step in this pathway is catalyzed by ATP sulfurylase, which synthesizes adenosine 5'-phosphosulfa

138 The expressed monofunctional ATP-sulfurylase, which was initially fully active, was unsta

139 Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer appro

140 he reported crystal structures of fungal ATP sulfurylases, which contained bound substrates, but it i