ライフサイエンスコーパス: tRNA(Ser)

1 mcd2-1, and show that the mutation lies in a tRNA(Ser)(CGA), which has been modified to translate the

2 We demonstrate that a tRNA(Ser(UCN)) precursor with the U7445C substitution ca

3 38 in the anticodon loop, only tRNA(Ser)AGA, tRNA(Ser)CGA, tRNA(Ser)UGA, and selenocysteine tRNA with

4 extent, differences in the in vivo tRNA(Ala):tRNA(Ser) ratio in 159 and Pn16.

5 This minimally altered tRNA(Ser) exclusively inserted leucine residues and was

6 His(GUG)) for Um, and tRNA(Pro(GGG)) for Am. tRNA(Ser(UGA)), previously observed as a TrmJ substrate

7 Although SerRS recognizes both tRNA(Sec) and tRNA(Ser) species, PSTK must discriminate Ser-tRNA(Sec)

8 condary structures of archaeal tRNA(Sec) and tRNA(Ser), we introduced mutations into Methanococcus ma

9 a unique location between the tRNA(His) and tRNA(Ser (AGY)) genes.

10 ends) analyses indicated that the four-armed tRNASer(UCN) gene is transcribed into a stable RNA that

11 cates that similarity between the four-armed tRNASer(UCN) genes is only 63.8% compared with an averag

12 he M. californianus and M. edulis four-armed tRNASer(UCN) sequences are interpreted as pseudo-tRNASer

13 ylation of variant transcripts of M. barkeri tRNASer was kinetically analyzed in vitro with pure enzy

14 ntity elements into the mitochondrial bovine tRNA(Ser) scaffold yielded chimeric tRNAs active both in

15 codon loop, only tRNA(Ser)AGA, tRNA(Ser)CGA, tRNA(Ser)UGA, and selenocysteine tRNA with UCA (tRNA([Se

16 1--72 through 5--68 base pairs of the E.coli tRNA(Ser) acceptor stem with the major recognition eleme

17 precision values for the analyses of E. coli tRNASer(VGA) and E. coli tRNAThr(GGU), unfractionated tR

18 Dihydrouridine content of Escherichia coli tRNASer(VGA) and tRNAThr(GGU) as controls were measured

19 d by the DHU arm-replacement loop-containing tRNASer(UCN), tRNASer(AGN), tRNAMet(AUA), tRNATrp, and t

20 ation of unspliced precursor RNAs of dimeric tRNA(Ser)-tRNA(Met)i, suggesting a novel nuclear role fo

21 nt of 28 isolated mutants contain duplicated tRNA(Ser)UCA-C47:6U genes.

22 four- to fivefold excess over the endogenous tRNA(Ser).

23 cations, we used a genetic screen to examine tRNA(Ser(CGA)) variants.

24 DNA sequence analysis of cDNA clones for tRNA(Ser) and 18S rRNA confirmed the expected 3'-termina

25 tigate the requirements of these enzymes for tRNASer recognition, serylation of variant transcripts o

26 eotides at the base of the variable stem for tRNASer recognition, unlike its bacterial type counterpa

27 NA(Sec) were crucial for discrimination from tRNA(Ser).

28 jannaschii PSTK distinguishes tRNA(Sec) from tRNA(Ser).

29 ich structural or sequence elements of human tRNA(Ser) are necessary for pseudouridine (Psi) formatio

30 y, carrot protoplasts transfected with human tRNA(Ser)AUC genes containing the lac operator (lacO) in

31 o acid identity and recognition of a type II tRNA(Ser) amber suppressor from a serine to a leucine re

32 Furthermore, the A5-U68 base pair in tRNA(Ser) has some antideterminant properties for PSTK.

33 ; the G15-C48 tertiary "Levitt base-pair" in tRNA(Ser) was changed to A15-U48; the number of nucleoti

34 ed to a disproportionately large increase in tRNA(Ser)UCA-C47:6U levels in sla1-rrm but not sla1-null

35 uch a distinction between the two enzymes in tRNASer identity determinants reflects their evolutionar

36 We discuss how the existence of a large tRNA(Ser) gene family may permit this suppression withou

37 mutants lacking m(7)G and m(5)C, and mature tRNA(Ser(CGA)) in mutants lacking Um and ac(4)C.

38 Nase P, was found to process a mitochondrial tRNA(Ser(UCN)) precursor [ptRNA(Ser(UCN))] at the correc

39 ion of the deafness-associated mitochondrial tRNA(Ser(UCN)) T7511C mutation, in conjunction with homo

40 addition to the non-canonical mitochondrial tRNA(Ser(AGY)), but no obvious qualitative differences i

41 utations investigated in human mitochondrial tRNA(Ser(UCN)) affect processing efficiency, and some af

42 Mammalian mitochondrial tRNA(Ser(UCN)) (mt-tRNA(Ser)) and pyrrolysine tRNA (tRNA

43 n patient fibroblasts rendered mitochondrial tRNA(Ser(AGY)) undetectable, and markedly reduced mitoch

44 from defective CCA addition to mitochondrial tRNA(Ser(AGY)), and that the severity of this biochemica

45 s) gene at its 5' end and by 23 bp of the mt tRNA(Ser (AGY)) gene at its 3' end.

46 ([Ser]Sec)UCA level was increased and the mt tRNA(Ser)UGA level was decreased, suggesting that TRIT1

47 Furthermore, in human mt-tRNA(Ser), lengthening the variable loop by the 7472insC

48 ts confirm that insertion mutations lower mt-tRNA(Ser) melting temperature by 6-9 degrees C and incre

49 traints and hence destabilizes the mutant mt-tRNA(Ser) by approximately 0.6 kcal/mol relative to wild

50 ble loop by the 7472insC mutation reduces mt-tRNA(Ser) concentration in vivo through poorly understoo

51 e unique secondary structure of wild-type mt-tRNA(Ser) decrease the entropic cost of folding by appro

52 Mammalian mitochondrial tRNA(Ser(UCN)) (mt-tRNA(Ser)) and pyrrolysine tRNA (tRNA(Pyl)) fold to near

53 duplication and loss of function, and a new tRNA(Ser(UCN)) gene has been created de novo.

54 in addition the G73 "discriminator" base of tRNA(Ser) was changed to A73.

55 The substrate, composed of tRNA(Ser) and tRNA(Leu), is transcribed in tandem with a

56 t in complex with the anticodon stem loop of tRNA(Ser) bound to the PsiAG stop codon in the A site.

57 such as the purine-pyrimidine base pairs of tRNA(Ser).

58 Some point mutations in the ASL stem of tRNA(Ser) had significant effects on the levels of modif

59 -loop are seen in the cocrystal structure of tRNA(Ser) and Thermus thermophilus seryl-tRNA synthetase

60 directly following the discriminator base of tRNA(Ser(UCN))) causes non-syndromic deafness.

61 mutation that disrupts the anticodon stem of tRNA(Ser)CGA require Lhp1p for growth.

62 ta show a sharp threshold in the capacity of tRNASer(UCN) to support the wild-type protein synthesis

63 21 derived from four isoacceptor families of tRNASer genes, 7 from five families of tRNALeu genes, an

64 two SerRSs do not possess a uniform mode of tRNASer recognition, and additional determinants are nec

65 quence A36A37A38 in the anticodon loop, only tRNA(Ser)AGA, tRNA(Ser)CGA, tRNA(Ser)UGA, and selenocyst

66 Although maturation of the mutant pre-tRNA(Ser)CGA requires Lhp1p, introduction of a second mu

67 Suppressor pre-tRNA(Ser)UCA-C47:6U with a debilitating substitution in

68 We demonstrate that for the wild-type pre-tRNA(Ser)CGA and other pre-tRNAs, Lhp1p is required for

69 Ser(UCN) sequences are interpreted as pseudo-tRNASer(UCN) genes.

70 o distinguish tRNA(Sec) from closely related tRNA(Ser) substrate.

71 ucture, while two [tRNASer(AGN) and a second tRNASer(UCN)] will fold only into tRNAs with a dihydrour

72 STK must discriminate Ser-tRNA(Sec) from Ser-tRNA(Ser).

73 reover, a novel determinant for the specific tRNASer recognition was identified as the anticodon stem

74 ion to the point where the ochre-suppressing tRNA(Ser) is in four- to fivefold excess over the endoge

75 ppression by the weak ochre (UAA) suppressor tRNA(Ser) SUQ5.

76 antation of these identity elements into the tRNA(Ser)(UGA) scaffold resulted in phosphorylation of t

77 Deletion analysis showed that the tRNA(Ser) TPsiC stem-loop was a determinant for modifica

78 (SerDelta); an amber suppressor in which the tRNA(Ser) type II extra-stem-loop is replaced by a conse

79 However, in the eremobatid, the tRNA(Ser(UCN)) gene in the repeat region appears to have

80 This amount of reduction in the tRNA(Ser(UCN)) level is below a proposed threshold to su

81 exhibited approximately 75% decrease in the tRNA(Ser(UCN)) level, compared with three control cybrid

82 when lacO was located at position -1 of the tRNA(Ser)AUC coding sequence.

83 sequence analyses found a duplication of the tRNA(Ser)UCA-C47:6U gene, which was shown to cause the p

84 verage reduction of approximately 70% in the tRNASer(UCN) level and a decrease of approximately 45% i

85 e that the mutation flanks the 3' end of the tRNASer(UCN) gene sequence and affects the rate but not

86 7 kbp upstream and is cotranscribed with the tRNASer(UCN) gene, with strong evidence pointing to a me

87 cture of the homologous Thermus thermophilus tRNA(Ser)-SerRS complex that Cusack and colleagues repor

88 -encoded tRNAs with A36A37A38, only mt tRNAs tRNA(Ser)UGA and tRNA(Trp)UCA contained detectable i6A37

89 r-armed tRNA secondary structure, while two [tRNASer(AGN) and a second tRNASer(UCN)] will fold only i

90 ins 27 tandem repeats of tRNA(Tyr)-tRNA(Tyr)-tRNA(Ser) genes.

91 rm-replacement loop-containing tRNASer(UCN), tRNASer(AGN), tRNAMet(AUA), tRNATrp, and tRNAPro genes o

92 eit at a lower level than tRNA(Sec), whereas tRNA(Ser) did not.

93 ic tRNAs are charged at >80% levels, whereas tRNASer and tRNAThr are charged at lower levels.

94 Moreover, while tRNA(Ser) levels were unaffected by TRIT1 knockdown, the