ライフサイエンスコーパス: yeast two-hybrid system

1 l interaction of full-length PsIAA4 in vivo (yeast two-hybrid system).

2 followed by phenotypic screening based on a yeast two-hybrid system.

3 p6, a human cDNA library was screened in the yeast two-hybrid system.

4 gion of AbetaPP have been isolated using the yeast two-hybrid system.

5 e amino-terminal end of P. yoelii MSP-1 in a yeast two-hybrid system.

6 vestigated the interaction directly with the yeast two-hybrid system.

7 proteins that interacted with p35 using the yeast two-hybrid system.

8 ct with each other and self-associate in the yeast two-hybrid system.

9 f the IFT complex was investigated using the yeast two-hybrid system.

10 ns of VacA (termed p-33 and p-55) by using a yeast two-hybrid system.

11 1p as trap were active when tested using the yeast two-hybrid system.

12 apacity to mediate heterodimerization in the yeast two-hybrid system.

13 a cDNA library via a variant of the original yeast two-hybrid system.

14 ize the 4OHT-bound ERalpha conformation in a yeast two-hybrid system.

15 mbrane protein was investigated by using the yeast two-hybrid system.

16 pping of protein-protein interactions is the yeast two-hybrid system.

17 tation, as well as genetic tools such as the yeast two-hybrid system.

18 azE and MazF was also characterized with the yeast two-hybrid system.

19 with actin was originally characterized by a yeast two-hybrid system.

20 s of mouse Rpgr(ORF15) was used as bait in a yeast two-hybrid system.

21 important protein interactions has been the yeast two-hybrid system.

22 nable to interact with wild-type p-55 in the yeast two-hybrid system.

23 ssayed and found to interact strongly in the yeast two-hybrid system.

24 NifL interacted with GlnK and GlnKY51F in a yeast two-hybrid system.

25 terminal regulatory region of p100 using the yeast two-hybrid system.

26 with the Cdh1 substrate-binding protein in a yeast two-hybrid system.

27 sigma(28) was demonstrated by utilizing the yeast two-hybrid system.

28 s factor receptor family, as the bait in the yeast two-hybrid system.

29 protein-protein interaction assays using the yeast two-hybrid system.

30 t with SKR-1, -2, -3, -7, -8, and -10 in the yeast two-hybrid system.

31 lpha-, beta-, gamma-, and delta-zeins in the yeast two-hybrid system.

32 cell complementary DNA library by using the yeast two-hybrid system.

33 n p29 and p67(phox) was demonstrated using a yeast two-hybrid system.

34 16 interacts with AtbZIP68 and AtGBF1 in the yeast two-hybrid system.

35 cDNA expression library was screened using a yeast two-hybrid system.

36 e direct interaction of FleN and FleQ in the yeast two-hybrid system.

37 is interaction was initially found using the yeast two-hybrid system.

38 ed significant dimer formation, by using the yeast two-hybrid system.

39 thermore, LIN-56 and LIN-15A interact in the yeast two-hybrid system.

40 MYOC-MYOC interactions were studied with a yeast two-hybrid system.

41 ibrary for interacting proteins by using the yeast two-hybrid system.

42 necessary for interaction with Src using the yeast two-hybrid system.

43 are confirmed by similar interactions in the yeast two-hybrid system.

44 ts were used to assay for interaction in the yeast two-hybrid system.

45 Munc18-1 was also identified using the yeast two-hybrid system.

46 protein, UBL1, associates with RAD51 in the yeast two-hybrid system.

47 techniques such as mass spectrometry or the yeast two-hybrid system.

48 boxyl terminus (beta(1)AR-CT) as bait in the yeast two-hybrid system.

49 cific binding domains within MUC5B using the yeast two-hybrid system.

50 expression library and screened it using the yeast two-hybrid system.

51 protein, WTAP, which was isolated using the yeast two-hybrid system.

52 of associating with the core protein using a yeast two-hybrid system.

53 it eliminated MucA-MucB interactions in the yeast two-hybrid system.

54 he interaction between these subunits in the yeast two-hybrid system.

55 ntify novel interactors of Smads by use of a yeast two-hybrid system.

56 coding 12-LOX interacting proteins using the yeast two-hybrid system.

57 e proapoptotic BCL-2-related proteins in the yeast two-hybrid system.

58 Ctr9 as a novel DAT binding partner using a yeast two-hybrid system.

59 efective for multimerization using a reverse yeast two-hybrid system.

60 ins using a highly specific, high-throughput yeast two-hybrid system.

61 binding site, CudA forms a homodimer in the yeast two-hybrid system.

62 eracts directly with the FANCD2 protein in a yeast two-hybrid system.

63 y developed a cost-effective high-throughput yeast two-hybrid system.

64 red for Msi1p to associate with Cac1p in the yeast two-hybrid system.

65 ssessed by using a stringent high-throughput yeast two-hybrid system.

66 d to the Z ring or interact with FtsZ in the yeast two-hybrid system.

67 alpha(2)-Heremans-Schmid glycoprotein in the yeast two-hybrid system.

68 domain in a membrane-bound, split-ubiquitin yeast two-hybrid system.

69 the 22-kDa alpha-zein when expressed in the yeast two-hybrid system.

70 brane anchor in MinD interactions, using the yeast two-hybrid system.

71 ding Drosophila PP1c-binding proteins in the yeast two-hybrid system.

72 E(77-114), in the N terminus of BfpE using a yeast two-hybrid system.

73 PNGase (mPNGase) were detected by using the yeast two-hybrid system.

74 vitro in affinity chromatography and in the yeast two-hybrid system.

75 transcriptional activation and expression in yeast two-hybrid systems.

76 Using a yeast two-hybrid system, 38 candidates interacting with

77 in-protein interaction assays done using the yeast two-hybrid system, 56 (approximately 17%) showed p

78 We report that in the yeast two-hybrid system a domain of U(S)3 essential for

79 he present study we have isolated, using the yeast two-hybrid system, a 182 amino acid residue fragme

80 screen a human liver cDNA library using the yeast two-hybrid system, a cDNA for cytohesin-1, a appro

81 We use the well studied yeast two-hybrid system adapted for mammalian cells and

82 HPS1 and HPS4 do not interact directly in a yeast two-hybrid system, although HPS4 interacts with it

83 Through a yeast two-hybrid system, an in vitro binding assay, and

84 associated polypeptides using the Gal4-based yeast two-hybrid system and a cDNA library derived from

85 Using the yeast two-hybrid system and a heterologous cell system,

86 Using the yeast two-hybrid system and a protein array membrane, we

87 Using the yeast two-hybrid system and affinity immobilization assa

88 een revealed by the RNAi assays, we used the yeast two-hybrid system and an in vitro glutathione-S-tr

89 Using the yeast two-hybrid system and bimolecular fluorescence com

90 ms of the importin alpha protein family in a yeast two-hybrid system and by an in planta bimolecular

91 5Delta32, both by genetic criteria using the yeast two-hybrid system and by biochemical criteria usin

92 interaction was confirmed in a conventional yeast two-hybrid system and by direct interaction betwee

93 In a novel application of the yeast two-hybrid system and by immunoprecipitation, we s

94 To explain this process, we show by a yeast two-hybrid system and chemical cross-linking that

95 We used the yeast two-hybrid system and co-immunoprecipitation analy

96 nteraction between Rad52 and Rad59 using the yeast two-hybrid system and co-immunoprecipitation from

97 Using the yeast two-hybrid system and co-immunoprecipitation metho

98 Using both the yeast two-hybrid system and coimmunoprecipitation assays

99 teraction was initially demonstrated using a yeast two-hybrid system and corroborated by both in vivo

100 Using the yeast two-hybrid system and domain III of perlecan as ba

101 ns on nibrin and Mre11 were mapped using the yeast two-hybrid system and expression of epitope-tagged

102 we screened a prostate cDNA library using a yeast two-hybrid system and found that the cleavage and

103 K1 as a binding partner for UNC5H1 using the yeast two-hybrid system and found that the extreme three

104 etween decorin and EGFR was confirmed in the yeast two-hybrid system and further validated by experim

105 We used the yeast two-hybrid system and gel overlays to study intimi

106 X proteins was shown using a split ubiquitin yeast two-hybrid system and gel shift assays.

107 with the cytoplasmic tail of megalin in the yeast two-hybrid system and glutathione-S-transferase pu

108 We utilized a modified yeast two-hybrid system and identified a new, widely exp

109 to the cytoplasmic domain of ADAM12 using a yeast two-hybrid system and identified a protein called

110 with Nck via the intermediate domain in the yeast two-hybrid system and in a glutathione S-transfera

111 eIF4H interacts physically with eIF4A in the yeast two-hybrid system and in GST pull-down assays and

112 racted with Agrobacterium protein VirE2 in a yeast two-hybrid system and in planta.

113 ved C-terminal motif in Hook proteins in the yeast two-hybrid system and in tissue culture cells, and

114 ptide also interacts with OASTL based on the yeast two-hybrid system and in vitro binding assays.

115 KorB and IncC interact in vivo by using the yeast two-hybrid system and in vitro by using partially

116 spore coat protein, SafA, in vivo using the yeast two-hybrid system and in vitro.

117 that it directly associates with maspin in a yeast two-hybrid system and in vitro.

118 nitially developed interaction assays (e.g., yeast two-hybrid system and split-ubiquitin assay) usual

119 hways of maspin, we employed a maspin-baited yeast two-hybrid system and subsequently identified Inte

120 d proteins that interacted with INSM1 by the yeast two-hybrid system and the binding of one of them,

121 We employed the yeast two-hybrid system and used perlecan domain V as ba

122 beta-galactosidase quantitative assay in the yeast two-hybrid system and were confirmed by an in vitr

123 Using the yeast-two hybrid system and coprecipitation of recombina

124 RsbQ bound RsbP in the yeast two-hybrid system, and a large in-frame deletion i

125 ain, Ckigamma2, -gamma3, and -epsilon in the yeast two-hybrid system, and bound Ckidelta and -epsilon

126 , perfluoro-octanoate-PAGE, a membrane-based yeast two-hybrid system, and chemical cross-linking expe

127 3DX bound well to its epitope in a yeast two-hybrid system, and GST-fused 3DX also bound to

128 th the plant SnRK AKIN11 both in vivo in the yeast two-hybrid system, and in vitro in a GST-fusion 'p

129 s with the spliceosome protein U1-70K in the yeast two-hybrid system, and is co-localized with U1-70K

130 y lipoprotein complexes, was identified by a yeast two-hybrid system as a strong and specific binding

131 e 3 (PDCL3, also known as PhLP2A), through a yeast two-hybrid system, as a novel protein involved in

132 In a yeast two-hybrid system based on reconstitution of Ras s

133 Immunoprecipitation and the yeast two-hybrid system both suggest physical interactio

134 etween IDH1 and IDH2 were detected using the yeast two-hybrid system, but interactions between identi

135 ally impaired and unregulated CDPKs with the yeast two-hybrid system can accelerate the discovery of

136 Using the yeast two-hybrid system, CCTeta was found to bind to the

137 9 was studied using affinity chromatography, yeast two-hybrid system, coimmunoprecipitation, and gel

138 CDC-42 physically interacts with PAR-6 in a yeast two-hybrid system, consistent with data on the int

139 finity purification-mass spectrometry or the yeast two-hybrid system, contributes a unique and releva

140 We used the yeast two-hybrid system coupled with random mutagenesis

141 The yeast two-hybrid system data also indicated that CarR is

142 hermore, extensive assays utilizing the Gal4 yeast two-hybrid system demonstrate interactions of syne

143 Experiments using the yeast two-hybrid system demonstrated a protein-protein i

144 Use of the yeast two-hybrid system demonstrated direct interaction

145 In this review we will introduce the yeast two-hybrid system, discuss modifications of the sy

146 nst a library carrying M. xanthus DNA in the yeast two-hybrid system, eight positive, independent clo

147 breast epithelial cell cDNA library using a yeast two-hybrid system for ARHI-interacting proteins.

148 DNA library with a specific antibody and the yeast two-hybrid system for protein interaction using as

149 Since its original description in 1989, the yeast two-hybrid system has been extensively used to ide

150 In the membrane-based yeast two-hybrid system, homo-oligomeric interactions be

151 ons that occur in E. coli also occurs in the yeast two-hybrid system (i.e., off-DNA).

152 Using a yeast two-hybrid system, immunocolocalization, immunopre

153 lular domains of each immune receptor in the yeast two-hybrid system in a kinase activity-dependent m

154 les of human DNA ligase IV, we have used the yeast two-hybrid system in conjunction with traditional

155 By using the yeast two-hybrid system, in vitro coimmunoprecipitation,

156 ts, with the cytoplasmic portion of Ob-Rb in yeast two-hybrid systems, in protein precipitation exper

157 Additional mapping studies in the yeast two-hybrid system indicated that only the N-termin

158 The yeast two-hybrid system indicated that regions near the

159 Analysis in the yeast two-hybrid system indicates that the N-terminal po

160 the HEC proteins can dimerize with SPT in a yeast two-hybrid system, indicating that the HEC genes w

161 gp41 CD failed to interact with PRA1 in the yeast two-hybrid system, its interaction with PRA1 was s

162 ffinity-capture complex purification and the yeast two-hybrid system, may produce inaccurate data set

163 When tested in the yeast two-hybrid system, mutation of Leu-536 increased t

164 o experiments using either a split ubiquitin yeast two-hybrid system or bimolecular fluorescence comp

165 st, Tie1 did not interact with ABIN-2 in the yeast two-hybrid system or mammalian cells.

166 sduction pathways have successfully used the yeast two-hybrid system or related methods.

167 (1) Using the C-terminus of LPP as bait in a yeast two hybrid system, palladin, an actin-associated p

168 Using a modified yeast two-hybrid system, PDZ(Omi) mutants were isolated

169 By using the yeast two-hybrid system, purified lectin and pilin domai

170 Here we report that in the yeast two-hybrid system, Rad51D interacts with XRCC2 and

171 Since the first description of the yeast two-hybrid system, related genetic assays for prot

172 it to screen a human heart cDNA library in a yeast two-hybrid system, retrieving two unique clones th

173 Deletion analysis using the yeast two-hybrid system revealed that the armadillo repe

174 Although the yeast two-hybrid system suggested an interaction of six

175 Third, analysis in the yeast two-hybrid system suggested that all six paralogs

176 ble activation partners for SAF-1, we used a yeast two-hybrid system that detected interaction betwee

177 Evidence from the yeast two-hybrid system that the D4R and D5R proteins ca

178 However, MA showed binding to TSG101 in the yeast two-hybrid system that was dependent on an intact

179 Utilizing a split-ubiquitin membrane yeast two-hybrid system that was developed to identify i

180 ted in a physical interaction, we employed a yeast-two-hybrid system that revealed a dimerization bet

181 In yeast two-hybrid systems, the N-terminus of NIL-16 inter

182 cellular C terminus of the human CaR and the yeast two hybrid system to screen a human kidney cDNA li

183 We used the yeast two-hybrid system to analyze the role of Alpharetr

184 romatography, coimmunoprecipitation, and the yeast two-hybrid system to demonstrate that the extracel

185 We then used a yeast two-hybrid system to detect potential protein-prot

186 Using a yeast two-hybrid system to detect protein-protein intera

187 We have used the yeast two-hybrid system to detect variants of GlnB that

188 In this study, we used the yeast two-hybrid system to determine the multimerization

189 In the present study, we used the yeast two-hybrid system to determine the site of interac

190 Using the yeast two-hybrid system to determine the target host cel

191 We have modified the yeast two-hybrid system to enable the detection of prote

192 of cellular signaling, we have modified the yeast two-hybrid system to explore the possibility of NO

193 which was used in this report as bait in the yeast two-hybrid system to find other interacting cell t

194 In this study we used the yeast two-hybrid system to find proteins interacting wit

195 We used a yeast two-hybrid system to identify a putative cotton fi

196 In this study, we have used the yeast two-hybrid system to identify an ETS1 interacting

197 m of AR-regulated transcription, we used the yeast two-hybrid system to identify AR-associated protei

198 We used the yeast two-hybrid system to identify binding partners of

199 clues about the function of APP, we used the yeast two-hybrid system to identify interaction between

200 Here we use a yeast two-hybrid system to identify novel TIR1 mutants w

201 regulation of PKCtheta, we have employed the yeast two-hybrid system to identify PKCtheta-interacting

202 Use of the yeast two-hybrid system to identify proteins that associ

203 beta PP processing and function, we used the yeast two-hybrid system to identify proteins that intera

204 We used the yeast two-hybrid system to identify proteins that intera

205 We have used the yeast two-hybrid system to identify proteins that intera

206 In this study, we have used the yeast two-hybrid system to identify proteins that intera

207 ignal transduction pathway, we have used the yeast two-hybrid system to identify proteins that physic

208 d random mutagenesis in combination with the yeast two-hybrid system to identify residues in the YopH

209 Here we have used the yeast two-hybrid system to identify the proteins that in

210 We used the yeast two-hybrid system to identify the Prtb (Proline-ri

211 an integrated strategy based on the reverse yeast two-hybrid system to isolate and characterize such

212 We have used the yeast two-hybrid system to isolate Nova interacting prot

213 Because of the failure of the yeast two-hybrid system to reveal interactions between l

214 We have employed a yeast two-hybrid system to screen a B lymphoblast-derive

215 ial targets of c-Abl kinase, we utilized the yeast two-hybrid system to screen a murine cDNA library.

216 Using the era gene as bait in the yeast two-hybrid system to screen E. coli genomic librar

217 We developed a sensitive yeast two-hybrid system to screen for hERalpha variants

218 phosphorylation, we modified the Gal4-based yeast two-hybrid system to screen for phosphorylation-de

219 s of BCSG1 in breast cancer cells, we used a yeast two-hybrid system to screen for proteins that coul

220 or cell invasion and metastasis, we used the yeast two-hybrid system to screen for proteins that inte

221 We used an optimized yeast two-hybrid system to screen mouse pregnancy-associ

222 Here, using a yeast two-hybrid system to search for AtRALF1-interactin

223 Thus, we screened a cDNA library using a yeast two-hybrid system to search for interacting protei

224 results also demonstrate the utility of the yeast two-hybrid system to study protein-protein interac

225 Here we have used the yeast two-hybrid system to test for direct interaction b

226 FBP-5, we screened a human cDNA library by a yeast two-hybrid system using IGFBP-5 as bait and identi

227 In the present study, we employed yeast two-hybrid system using the N-terminal domain of A

228 brain microvascular endothelial cells by the yeast two-hybrid system using the N-terminal domain of C

229 ing with Rb in muscle cells, we utilized the yeast two-hybrid system, using the A-B and C pockets of

230 interaction was identified by screening of a yeast two-hybrid system vascular endothelial cell librar

231 The yeast two hybrid system was used to confirm these intera

232 ential nucleator of enamel crystallites, the yeast two-hybrid system was applied to a mouse tooth exp

233 the role of RGSZ1 in cellular signaling, the yeast two-hybrid system was employed to identify potenti

234 MP, a functional genetic screen based on the yeast two-hybrid system was performed.

235 A yeast two-hybrid system was used to analyze interactions

236 Yeast two-hybrid system was used to demonstrate that a h

237 In addition, a yeast two-hybrid system was used to detect the interacti

238 The yeast two-hybrid system was used to identify a groucho h

239 The yeast two-hybrid system was used to isolate the ATP bind

240 The yeast two-hybrid system was used to search for proteins

241 biguous interaction, first observed with the yeast two-hybrid system, was corroborated by co-immunopr

242 Using the yeast two-hybrid system we have identified an RNA-bindin

243 Using that domain as bait in a yeast two-hybrid system we now report the identification

244 Using the yeast-two hybrid system we isolated a novel Numb interac

245 Using the yeast two hybrid system, we show that the iaa17/axr3-1 m

246 Using the yeast two-hybrid system, we cloned an apolipoprotein (ap

247 entifying Jak2-interacting proteins with the yeast two-hybrid system, we cloned the human homologue o

248 ys, radioligand binding experiments, and the yeast two-hybrid system, we demonstrate that FGF7 binds

249 Using a yeast two-hybrid system, we found that both SPECs intera

250 Using the yeast two-hybrid system, we found that HAP1 also interac

251 Using the yeast two-hybrid system, we found that these mutations d

252 Using the yeast two-hybrid system, we found that TRIM32 binds and

253 Using the yeast two-hybrid system, we found that VZV IE63 interact

254 In the present study employing a yeast two-hybrid system, we found that zyxin, a molecule

255 Using the yeast two-hybrid system, we have identified a human cell

256 In this report, using the yeast two-hybrid system, we have identified a novel inte

257 Using the yeast two-hybrid system, we have identified a novel inte

258 By using the yeast two-hybrid system, we have identified a novel memb

259 kinase 1 (Plk1)-interacting proteins using a yeast two-hybrid system, we have identified histone acet

260 Using the yeast two-hybrid system, we have now identified two sper

261 Using the yeast two-hybrid system, we have observed a strong inter

262 Using yeast two-hybrid system, we have previously identified C

263 Using the yeast two-hybrid system, we here report an interaction b

264 Using a yeast two-hybrid system, we identified a calmodulin-rela

265 By using a yeast two-hybrid system, we identified a gene, invasion

266 Using the yeast two-hybrid system, we identified a novel calcineur

267 Using the yeast two-hybrid system, we identified a number of prote

268 Using the yeast two-hybrid system, we identified a transcriptional

269 Using the yeast two-hybrid system, we identified an interaction be

270 Using a yeast two-hybrid system, we identified Arabidopsis thali

271 may underlie the disease phenotype.Using the yeast two-hybrid system, we identified ETO/MTG8, a compo

272 With the use of the yeast two-hybrid system, we identified filamin-A (an act

273 Using the yeast two-hybrid system, we identified Hsp90, a chaperon

274 Using the yeast two-hybrid system, we identified importin alpha1 (

275 Utilizing the yeast two-hybrid system, we identified protein phosphata

276 Using the yeast two-hybrid system, we identified several second-si

277 Using the yeast two-hybrid system, we identified Sprouty2 as an in

278 Using the yeast two-hybrid system, we identified uridine kinase li

279 Using the yeast two-hybrid system, we isolated a membrane-associat

280 Using DAZ as bait in a yeast two-hybrid system, we isolated two DAZAP (DAZ-asso

281 Using the yeast two-hybrid system, we previously isolated a novel

282 Using a yeast two-hybrid system, we screened for galectin-3-inte

283 Using chemical cross-linking and the yeast two-hybrid system, we show that sortilin interacts

284 Using the yeast two-hybrid system, we show that the Mod(mdg4) prot

285 Using a yeast two-hybrid system, we show that these SOS2-like ki

286 With the use of the yeast two-hybrid system, we show that this Dnm1p oligome

287 Using a stringent, high-throughput yeast two-hybrid system, we tested pairwise interactions

288 ne was changed to alanine could activate the yeast two-hybrid system when paired with RsbW, whereas m

289 s with the Aspergillus NUDE coiled-coil in a yeast two-hybrid system, while human LIS1 interacts with

290 cer-associated C terminus (BRCT) domain in a yeast two-hybrid system, while increased sensitivity of

291 Here we report the cloning, by the yeast two hybrid system with dominant negative caspase-2

292 enesis, Pipkz1, was shown to interact in the yeast two-hybrid system with a putative bZIP transcripti

293 factor, interleukin-1, and ROS, we used the yeast two-hybrid system with ASK1 as bait to identify AS

294 on factor-1gamma (EF1gamma) interacts in the yeast two-hybrid system with DOA, the LAMMER protein kin

295 d caveolin-1 was determined by the Pro-Quest yeast two-hybrid system with full-length NSP4 and seven

296 Combining the yeast two-hybrid system with genetic analysis, we show h

297 The yeast two-hybrid system with myocilin as the bait and a

298 Using the yeast two-hybrid system with syntaxin-1A as bait, we iso

299 co-regulatory proteins in RKO cells using a yeast two-hybrid system with the intact TRbeta1 as bait.

300 g sites for VHb on the cytochrome, using the yeast two-hybrid system with VHb as the bait and testing