ライフサイエンスコーパス: zymogen granule

1 supporting a role in protein sorting to the zymogen granule.

2 ypoplastic and individual acini produced few zymogen granules.

3 2 may mediate the sequestration of zinc into zymogen granules.

4 creatic cancer cell line, MIA PaCa-2 without zymogen granules.

5 hat are predominantly associated with mature zymogen granules.

6 ding proteins and is localized on pancreatic zymogen granules.

7 ed that GP2 is important in the formation of zymogen granules.

8 nstrated the presence of Rab3D on pancreatic zymogen granules.

9 protein showed wild type Rab3D localized to zymogen granules.

10 us fluorescence imaging of quinacrine-loaded zymogen granules.

11 ity of the Gq/11 protein immunopurified from zymogen granules.

12 try, myosin I was also localized on isolated zymogen granules.

13 ting GTPase activity of the Gq/11 protein on zymogen granules.

14 -like protein to the cytoplasmic face of the zymogen granules.

15 beta subunits were not found on membranes of zymogen granules.

16 ed with disorganized and dilated ER, loss of zymogen granules, accumulation of autophagic vacuoles, a

17 on the total GTPase activity of the purified zymogen granules and a larger inhibitory effect on the G

18 The acinar cells lose their zymogen granules and approximately 75% of their RNA.

19 to stimulate the GTPase activity of isolated zymogen granules and cause swelling.

20 y involves SNARE and Rab proteins present on zymogen granules and cellular membrane domains.

21 Ca signals ultimately induce exocytosis of zymogen granules and identification of the proteins invo

22 in Gq/11 was identified on pancreatic acinar zymogen granules and its function in calcium-regulated e

23 ansporter ZnT2 (Slc30a2) is localized to the zymogen granules and that dietary zinc restriction in mi

24 t that only myosin I was present on isolated zymogen granules and their membranes.

25 in mice decreased the zinc concentration of zymogen granules and ZnT2 expression.

26 olocalized with glycoprotein 2, a marker for zymogen granules, and actin.

27 as degeneration of exocrine cells, decreased zymogen granules, and alterations in the endoplasmic ret

28 teins found on synaptic vesicles, pancreatic zymogen granules, and chromaffin granules, suggests GAIP

29 Pancreatic acini are completely devoid of zymogen granules, and the ER is severely distended.

30 t the amount of endogenous Rab3D on purified zymogen granules as assessed by either Western blotting

31 etagogue carbamylcholine, a subpopulation of zymogen granules became coated with filamentous actin.

32 ced in the pancreas where they are stored in zymogen granules before secretion into the intestine.

33 h Rap1 and Epac1 colocalized with amylase in zymogen granules, but only Rap1 was integral to the zymo

34 Hemagglutinin-tagged Rab3D was localized to zymogen granules by immunohistochemistry, and was shown

35 ycerol, and cAMP activate the release of the zymogen granule content in a manner that is poorly under

36 d contrast to the distribution of amylase, a zymogen granule content protein.

37 in bound directly at mildly acidic pH to the zymogen granule content proteins amylase, prolipase, pro

38 nule size, appearance, or the composition of zymogen granule contents.

39 It is concluded that zymogen granules do not express InsP3 receptors and thus

40 These data suggest that zymogen granules engaging in exocytosis become coated wi

41 in understanding the proteins present on the zymogen granules, especially Rabs and SNARE proteins, an

42 we describe characteristics of fusion during zymogen granule exocytosis in exocrine pancreatic acinar

43 the Ca(2+)-sensitive regulatory pathway for zymogen granule exocytosis.

44 y, we have investigated the possibility that zymogen granules express InsP3 receptors and are thus Ca

45 timulation, a process that may contribute to zymogen granule formation.

46 sential for pancreatic exocrine secretion or zymogen granule formation.

47 In a zymogen granule fraction prepared in an identical manner

48 Zymogen granules in K8/K18 pancreata were 50% smaller an

49 l cellular polarity and inability to secrete zymogen granules in pancreatic acinar exocrine cells.

50 Zymogen granules in the GP2 knock-out mice appeared norm

51 g disruption of retinal cell layers, lack of zymogen granules in the pancreas, and dilated Golgi in i

52 n the process of transport and exocytosis of zymogen granules in the pancreatic acinar cell.

53 oad that results from the exocytic fusion of zymogen granules is significantly blunted by HCO3 (-) bu

54 ding to exocytosis of pancreatic acinar cell zymogen granules is the inositol 1,4,5-trisphosphate (In

55 also because the majority of them lacked the zymogen granule marker rab3D, a small GTPase implicated

56 not alphas or alphao, to be localized to the zymogen granule membrane along with G-protein beta-subun

57 stigation has focused on the proteins of the zymogen granule membrane, and several novel proteins hav

58 The immunolocalization of myosin I to zymogen granule membranes and its close association with

59 The small GTPase Rab27B localizes to the zymogen granule membranes and plays an important role in

60 stochemistry, and was shown to be present on zymogen granule membranes by Western blotting; both resu

61 f these v-SNARE proteins are associated with zymogen granule membranes in pancreatic acinar cells.

62 granules, but only Rap1 was integral to the zymogen granule membranes.

63 Within the acinar cell, Itmap1 localizes to zymogen granule membranes.

64 They were distinct from regular zymogen granules not only because of their association w

65 he pancreatic pro-enzymes, packaged into the zymogen granules of acinar cells, become activated and c

66 GP2 is the major membrane protein present in zymogen granules of the exocrine pancreas.

67 r, did not inhibit GTPase activity of either zymogen granules or immunopurified Gq/11.

68 ish apical-basal polarity, properly position zymogen granules, or communicate with adjacent cells, di

69 as significant keratin overexpression alters zymogen granule organization and causes aging-associated

70 We conclude that Rab3D protein on zymogen granules plays a stimulatory role in regulated a

71 We conclude that Gq/11 protein on zymogen granules plays a tonic inhibitory role in calciu

72 In the acinar cell nucleus, the zymogen granule region and contour of the cell were inte

73 pical-basal polarity, increasing the size of zymogen granules, reorganizing the cytoskeletal network,

74 bules showed that alphaq/11 localized to the zymogen granule-rich apical region of acinar cells toget

75 Itmap1 deficiency does not alter zymogen granule size, appearance, or the composition of

76 Ca2+ had no effect on zymogen granule size.

77 ion by blocking the GDP/GTP exchange but not zymogen granule targeting of endogenous Rab3D.

78 were able to study the dynamics of isolated zymogen granules, the secretory vesicles in exocrine pan

79 that while the wild-type Rab3D localized to zymogen granules, the two dominant negative mutants did

80 rticipates in zinc transport into pancreatic zymogen granules through a glucocorticoid pathway requir

81 Exposure of zymogen granules to GTP resulted in a 15-25% increase in

82 sms whereby intracellular messengers mediate zymogen granule transport and exocytosis in the pancreat

83 e entry of extracellular dye into individual zymogen granules undergoing exocytosis.

84 on in pancreatic acinar cells, exocytosis of zymogen granules was quantified by continuous, time-diff

85 These zymogen granules were always in proximity of the acinar

86 In contrast, when the zymogen granules were further purified on a Percoll grad

87 le areas containing no membranes (nuclei and zymogen granules) were not fluorescent.

88 le preparation consisted of essentially pure zymogen granules, whereas the granules prepared without

89 own about how Ca signals to induce fusion of zymogen granules with the apical plasma membrane.

90 We performed single zymogen granule (ZG) exocytosis assays, Ca(2+) imaging s

91 Acinar cell zymogen granules (ZG) express 2 isoforms of the vesicle-

92 ar cells contain two distinct populations of zymogen granules (ZGs) expressing either VAMP 2 or VAMP

93 treated) exhibit normal apical exocytosis of zymogen granules (ZGs) in response to physiologic stimul

94 Zymogen granules (ZGs), the membrane-bound secretory ves

95 lated amylase release and an accumulation of zymogen granules (ZGs).

96 in increased cytoplasmic zinc and decreased zymogen granule zinc that further demonstrated that ZnT2